Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystems
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/2945 |
Resumo: | Polyhydroxyalkanoates (PHAs) are biodegradable polyesters and environmentally friendly thermoplastics, which are accumulated as carbon and energy storage materials in various bacteria in limited growth conditions with excess carbon sources. In this study, bacteria were isolated from samples taken from various marine ecosystems in the Archipelago of Madeira in the Atlantic Ocean, and screened for their ability to accumulate polyhydroxyalkanoates. These samples were taken from the seabed at depths of 30 and 1,700 meters to obtain a larger diversity of microorganisms and therefore, in an attempt to obtain new structures of PHAs. Strains were directly isolated from 612 mother plates where marine samples had been initially plated. A total of 724 isolates from mother plates were obtained, of which 174 were found PHA-positive using Nile red viable-colony screening. All synthesized intracellular inclusions during growth on starch carbon source. Twenty-five bacterial isolates in 25 mL-scale cultivation were proven promising for PHA production with PHA storage maximum 17.71 % for MD12-107 and 9.30 % for MD12-581 strain. The inclusions were predominantly identified as poly-β-hydroxybutyrate (PHB) using gas chromatography. Strains MD12-107 and MD12-581 were tested in 100 mL-scale and bioreactor cultivation. The best results were achieved with strain MD12-581 accumulated PHA storage 15.40 % in less than 6.5 hours with 5.5 g/L of cell dry weight and a specific growth rate was 0.24 h-1 when grown in medium containing 40 g/L of starch, 8 g/L of yeast extract and 4 g/L of peptone during batch cultivation. |
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Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystemsPolyhydroxyalkanoatesNile blueNile redMarine bacteriaPolyhydroxyalkanoates (PHAs) are biodegradable polyesters and environmentally friendly thermoplastics, which are accumulated as carbon and energy storage materials in various bacteria in limited growth conditions with excess carbon sources. In this study, bacteria were isolated from samples taken from various marine ecosystems in the Archipelago of Madeira in the Atlantic Ocean, and screened for their ability to accumulate polyhydroxyalkanoates. These samples were taken from the seabed at depths of 30 and 1,700 meters to obtain a larger diversity of microorganisms and therefore, in an attempt to obtain new structures of PHAs. Strains were directly isolated from 612 mother plates where marine samples had been initially plated. A total of 724 isolates from mother plates were obtained, of which 174 were found PHA-positive using Nile red viable-colony screening. All synthesized intracellular inclusions during growth on starch carbon source. Twenty-five bacterial isolates in 25 mL-scale cultivation were proven promising for PHA production with PHA storage maximum 17.71 % for MD12-107 and 9.30 % for MD12-581 strain. The inclusions were predominantly identified as poly-β-hydroxybutyrate (PHB) using gas chromatography. Strains MD12-107 and MD12-581 were tested in 100 mL-scale and bioreactor cultivation. The best results were achieved with strain MD12-581 accumulated PHA storage 15.40 % in less than 6.5 hours with 5.5 g/L of cell dry weight and a specific growth rate was 0.24 h-1 when grown in medium containing 40 g/L of starch, 8 g/L of yeast extract and 4 g/L of peptone during batch cultivation.Os Polihidroxialcanoatos (PHAs) são biopoliésteres biodegradáveis e termoplásticos ecológicos, que são acumulados sob a forma de materiais de armazenamento de energia em várias bactérias em condições de crescimento limitado e fonte de carbono em excesso. Neste estudo, as bactérias foram isoladas a partir de amostras recolhidas a partir de vários ecossistemas marinhos no arquipélago da Madeira, no Oceano Atlântico e seleccionadas pela sua capacidade de acumular poli-hidroxialcanoatos. Estas amostras foram extraídas do fundo do mar a uma profundidade de 30 e 1,700 metros para se obter uma maior diversidade de microorganismos e, por conseguinte, numa tentativa de obter novas estruturas de PHAs. As estirpes foram directamente isoladas de 612 placas mãe onde as amostras marinhas tinham sido previamente espalhadas. Na totalidade foram isoladas 724 estirpes a partir das placasmãe, das quais 174 demonstraram resultado positivo durante o screening com o corante Vermelho do Nilo. Todas as estirpes com resultado positivo sintetizaram inclusões intracelulares durante o crescimento em amido como fonte de carbono. Vinte e cinco isolados bacterianos foram testados em cultura de 25 mL com resultados promissores quanto à produção de PHA com armazenamento de 17,71 % para a estirpe MD12-107 e 9,30 % para a estirpe MD12-581. As inclusões foram analisadas através da técnica de cromatografia gasosa como sendo predominantemente poli-β-hidroxibutirato (PHB). A produção a partir das estirpes MD12-107 e MD12-581 foi testada em 100 ml e em bioreactor. Os melhores resultados foram alcançados pela estirpe MD12-581 com um armazenamento de PHA de 15,40% em menos de 6,5 horas, com 5,5 g/L de concentração de biomassa e uma taxa específica de crescimento de 0,24 h-1 quando cultivada num meio contendo 40 g/L de amido, 8 g/L de extracto de levedura e 4 g/L de peptona durante a produção em bioreactor.Roca, ChristophePassarinha, Luís António PaulinouBibliorumBaptista, Silvia de Almeida2015-01-28T16:29:47Z2013-0620132013-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.6/2945enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:39:11Zoai:ubibliorum.ubi.pt:10400.6/2945Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:44:30.496995Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystems |
title |
Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystems |
spellingShingle |
Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystems Baptista, Silvia de Almeida Polyhydroxyalkanoates Nile blue Nile red Marine bacteria |
title_short |
Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystems |
title_full |
Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystems |
title_fullStr |
Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystems |
title_full_unstemmed |
Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystems |
title_sort |
Screening of polyhydroxyalkanoates producing bacteria isolated from marine ecosystems |
author |
Baptista, Silvia de Almeida |
author_facet |
Baptista, Silvia de Almeida |
author_role |
author |
dc.contributor.none.fl_str_mv |
Roca, Christophe Passarinha, Luís António Paulino uBibliorum |
dc.contributor.author.fl_str_mv |
Baptista, Silvia de Almeida |
dc.subject.por.fl_str_mv |
Polyhydroxyalkanoates Nile blue Nile red Marine bacteria |
topic |
Polyhydroxyalkanoates Nile blue Nile red Marine bacteria |
description |
Polyhydroxyalkanoates (PHAs) are biodegradable polyesters and environmentally friendly thermoplastics, which are accumulated as carbon and energy storage materials in various bacteria in limited growth conditions with excess carbon sources. In this study, bacteria were isolated from samples taken from various marine ecosystems in the Archipelago of Madeira in the Atlantic Ocean, and screened for their ability to accumulate polyhydroxyalkanoates. These samples were taken from the seabed at depths of 30 and 1,700 meters to obtain a larger diversity of microorganisms and therefore, in an attempt to obtain new structures of PHAs. Strains were directly isolated from 612 mother plates where marine samples had been initially plated. A total of 724 isolates from mother plates were obtained, of which 174 were found PHA-positive using Nile red viable-colony screening. All synthesized intracellular inclusions during growth on starch carbon source. Twenty-five bacterial isolates in 25 mL-scale cultivation were proven promising for PHA production with PHA storage maximum 17.71 % for MD12-107 and 9.30 % for MD12-581 strain. The inclusions were predominantly identified as poly-β-hydroxybutyrate (PHB) using gas chromatography. Strains MD12-107 and MD12-581 were tested in 100 mL-scale and bioreactor cultivation. The best results were achieved with strain MD12-581 accumulated PHA storage 15.40 % in less than 6.5 hours with 5.5 g/L of cell dry weight and a specific growth rate was 0.24 h-1 when grown in medium containing 40 g/L of starch, 8 g/L of yeast extract and 4 g/L of peptone during batch cultivation. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06 2013 2013-06-01T00:00:00Z 2015-01-28T16:29:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/2945 |
url |
http://hdl.handle.net/10400.6/2945 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136342167257088 |