Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communities
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/25582 https://doi.org/10.1111/gcb.12227 |
Resumo: | An experimental life support system (ELSS) was constructed to study the interactive effects of multiple stressors on coastal and estuarine benthic communities, specifically perturbations driven by global climate change and anthropogenic environmental contamination. The ELSS allows researchers to control salinity, pH, temperature, ultraviolet radiation (UVR), tidal rhythms and exposure to selected contaminants. Unlike most microcosms previously described, our system enables true independent replication (including randomization). In addition to this, it can be assembled using commercially available materials and equipment, thereby facilitating the replication of identical experimental setups in different geographical locations. Here, we validate the reproducibility and environmental quality of the system by comparing chemical and biological parameters recorded in our ELSS with those prevalent in the natural environment. Water, sediment microbial community and ragworm (the polychaete Hediste diversicolor) samples were obtained from four microcosms after 57 days of operation. In general, average concentrations of dissolved inorganic nutrients (NO3 ; NH4 + and PO4 3) in the water column of the ELSS experimental control units were within the range of concentrations recorded in the natural environment. While some shifts in bacterial community composition were observed between in situ and ELSS sediment samples, the relative abundance of most metabolically active bacterial taxa appeared to be stable. In addition, ELSS operation did not significantly affect survival, oxidative stress and neurological biomarkers of the model organism Hediste diversicolor. The validation data indicate that this system can be used to assess independent or interactive effects of climate change and environmental contamination on benthic communities. Researchers will be able to simulate the effects of these stressors on processes driven by microbial communities, sediment and seawater chemistry and to evaluate potential consequences to sediment toxicity using model organisms such as Hediste diversicolor. |
id |
RCAP_f2c1ca0be9b170268da9bc867ba8deae |
---|---|
oai_identifier_str |
oai:estudogeral.uc.pt:10316/25582 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communitiesbenthic communitiesclimate changemarine sedimentsmicrobial ecologymicrocosmpyrosequencingAn experimental life support system (ELSS) was constructed to study the interactive effects of multiple stressors on coastal and estuarine benthic communities, specifically perturbations driven by global climate change and anthropogenic environmental contamination. The ELSS allows researchers to control salinity, pH, temperature, ultraviolet radiation (UVR), tidal rhythms and exposure to selected contaminants. Unlike most microcosms previously described, our system enables true independent replication (including randomization). In addition to this, it can be assembled using commercially available materials and equipment, thereby facilitating the replication of identical experimental setups in different geographical locations. Here, we validate the reproducibility and environmental quality of the system by comparing chemical and biological parameters recorded in our ELSS with those prevalent in the natural environment. Water, sediment microbial community and ragworm (the polychaete Hediste diversicolor) samples were obtained from four microcosms after 57 days of operation. In general, average concentrations of dissolved inorganic nutrients (NO3 ; NH4 + and PO4 3) in the water column of the ELSS experimental control units were within the range of concentrations recorded in the natural environment. While some shifts in bacterial community composition were observed between in situ and ELSS sediment samples, the relative abundance of most metabolically active bacterial taxa appeared to be stable. In addition, ELSS operation did not significantly affect survival, oxidative stress and neurological biomarkers of the model organism Hediste diversicolor. The validation data indicate that this system can be used to assess independent or interactive effects of climate change and environmental contamination on benthic communities. Researchers will be able to simulate the effects of these stressors on processes driven by microbial communities, sediment and seawater chemistry and to evaluate potential consequences to sediment toxicity using model organisms such as Hediste diversicolor.This study was supported by the Centre for Environmental and Marine Studies (CESAM), the Foundation for Science and Technology (FCT, Portugal) PTDC/AAC-CLI/107916/2008 (http://alfa.fct.mctes.pt) and the European Regional Development Fund (ERDF) through COMPETE-(FCOMP-01-0124- FEDER-008657). Francisco J.R.C. Coelho and Rui J. M. Rocha were supported by a PhD scholarship (SFRH/BD/46322/2008 and SFRH/BD/46675/2008, respectively) funded by FCT (QREN-POPH – Type 4.1 – Advanced Training, subsidized by the European Social Fund and national funds MCTES). We thank Jos e Ferreira from EpArq, Aveiro University for graphical design of the ELSS.John Wiley & Sons, Ltd.2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/25582http://hdl.handle.net/10316/25582https://doi.org/10.1111/gcb.12227enghttp://onlinelibrary.wiley.com/doi/10.1111/gcb.12227/abstractCoelho, Francisco J. R. C.Rocha, Rui J. M.Pires, Ana C. C.Ladeiro, BrunoCastanheira, José M.Costa, RodrigoAlmeida, AdelaideCunha, ÂngelaLillebo, Ana I.Ribeiro, R.Pereira, RuthLopes, IsabelMarques, CatarinaMoreira-Santos, MatildeCalado, RicardoCleary, Daniel F. R.Gomes, Newton C. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-20T10:16:55Zoai:estudogeral.uc.pt:10316/25582Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:56:01.645144Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communities |
title |
Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communities |
spellingShingle |
Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communities Coelho, Francisco J. R. C. benthic communities climate change marine sediments microbial ecology microcosm pyrosequencing |
title_short |
Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communities |
title_full |
Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communities |
title_fullStr |
Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communities |
title_full_unstemmed |
Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communities |
title_sort |
Development and validation of an experimental life support system for assessing the effects of global climate change and environmental contamination on estuarine and coastal marine benthic communities |
author |
Coelho, Francisco J. R. C. |
author_facet |
Coelho, Francisco J. R. C. Rocha, Rui J. M. Pires, Ana C. C. Ladeiro, Bruno Castanheira, José M. Costa, Rodrigo Almeida, Adelaide Cunha, Ângela Lillebo, Ana I. Ribeiro, R. Pereira, Ruth Lopes, Isabel Marques, Catarina Moreira-Santos, Matilde Calado, Ricardo Cleary, Daniel F. R. Gomes, Newton C. M. |
author_role |
author |
author2 |
Rocha, Rui J. M. Pires, Ana C. C. Ladeiro, Bruno Castanheira, José M. Costa, Rodrigo Almeida, Adelaide Cunha, Ângela Lillebo, Ana I. Ribeiro, R. Pereira, Ruth Lopes, Isabel Marques, Catarina Moreira-Santos, Matilde Calado, Ricardo Cleary, Daniel F. R. Gomes, Newton C. M. |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Coelho, Francisco J. R. C. Rocha, Rui J. M. Pires, Ana C. C. Ladeiro, Bruno Castanheira, José M. Costa, Rodrigo Almeida, Adelaide Cunha, Ângela Lillebo, Ana I. Ribeiro, R. Pereira, Ruth Lopes, Isabel Marques, Catarina Moreira-Santos, Matilde Calado, Ricardo Cleary, Daniel F. R. Gomes, Newton C. M. |
dc.subject.por.fl_str_mv |
benthic communities climate change marine sediments microbial ecology microcosm pyrosequencing |
topic |
benthic communities climate change marine sediments microbial ecology microcosm pyrosequencing |
description |
An experimental life support system (ELSS) was constructed to study the interactive effects of multiple stressors on coastal and estuarine benthic communities, specifically perturbations driven by global climate change and anthropogenic environmental contamination. The ELSS allows researchers to control salinity, pH, temperature, ultraviolet radiation (UVR), tidal rhythms and exposure to selected contaminants. Unlike most microcosms previously described, our system enables true independent replication (including randomization). In addition to this, it can be assembled using commercially available materials and equipment, thereby facilitating the replication of identical experimental setups in different geographical locations. Here, we validate the reproducibility and environmental quality of the system by comparing chemical and biological parameters recorded in our ELSS with those prevalent in the natural environment. Water, sediment microbial community and ragworm (the polychaete Hediste diversicolor) samples were obtained from four microcosms after 57 days of operation. In general, average concentrations of dissolved inorganic nutrients (NO3 ; NH4 + and PO4 3) in the water column of the ELSS experimental control units were within the range of concentrations recorded in the natural environment. While some shifts in bacterial community composition were observed between in situ and ELSS sediment samples, the relative abundance of most metabolically active bacterial taxa appeared to be stable. In addition, ELSS operation did not significantly affect survival, oxidative stress and neurological biomarkers of the model organism Hediste diversicolor. The validation data indicate that this system can be used to assess independent or interactive effects of climate change and environmental contamination on benthic communities. Researchers will be able to simulate the effects of these stressors on processes driven by microbial communities, sediment and seawater chemistry and to evaluate potential consequences to sediment toxicity using model organisms such as Hediste diversicolor. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/25582 http://hdl.handle.net/10316/25582 https://doi.org/10.1111/gcb.12227 |
url |
http://hdl.handle.net/10316/25582 https://doi.org/10.1111/gcb.12227 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://onlinelibrary.wiley.com/doi/10.1111/gcb.12227/abstract |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
John Wiley & Sons, Ltd. |
publisher.none.fl_str_mv |
John Wiley & Sons, Ltd. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133845551841280 |