Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution

Detalhes bibliográficos
Autor(a) principal: Alves, Rafael
Data de Publicação: 2021
Outros Autores: Zetty-Arenas, Ana M., Demirci, H., Dias, Oscar, Rocha, I., Basso, Thiago O., Freitas, Sindelia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/68442
Resumo: Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.procbio.2020.11.013.
id RCAP_f2d11dfcb70be1c57c14eeb8c9fde1f9
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/68442
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolutionAdaptive laboratory evolutionHemicellulosic hydrolysateInhibtorsToleranceClostridium saccharoperbutylacetonicumGenomic analysisScience & TechnologySupplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.procbio.2020.11.013.In this study, adaptive laboratory evolution (ALE) was applied to isolate four strains of Clostridium saccharoperbutylacetonicum able to grow in the presence of hemicellulosic hydrolysate inhibitors unsupported by the parental strain. Among them, isolate RAC-25 presented the best fermentative performance, producing 22.1g/L of ABE and 16.7g/L of butanol. Genome sequencing revealed a deletion in the arabinose transcriptional repressor gene (araR) and a mutation in the anti-sigma factor I that promoted a downregulation of sigI. Gene expression analysis indicated high expression of genes related to H+-pumps (ATP synthases), proline biosynthesis (gamma phosphate reductase) and chaperonins (Grol), suggesting an integrated mechanism that is probably coordinated by the repression of sigI. Therefore, in addition to highlighting the power of ALE for selecting robust strains, our results suggest that sigI and araR may be interesting gene targets for increased tolerance toward inhibitor compounds relevant for lignocellulosic biofuels production.The authors would like to thank the Brazilian Center for Research in Energy and Materials (CNPEM) for providing access to the bioprocess facility of the Brazilian Biorenewables National Laboratory, and CNPq (400803/2013-5), FCT (UID/BIO/04469), BioTecNorte Operation (NORTE-01-0145-FEDER-000004) and Portuguese Biological Data Network” (ref. LISBOA-01-0145-FEDER-022231) for financial support.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoAlves, RafaelZetty-Arenas, Ana M.Demirci, H.Dias, OscarRocha, I.Basso, Thiago O.Freitas, Sindelia2021-022021-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/68442engAlves, Rafael; Zetty-Arenas, Ana M.; Demirci, H.; Dias, Oscar; Rocha, Isabel; Basso, Thiago O.; Freitas, Sindelia, Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution. Process Biochemistry, 101, 179-189, 20211359-511310.1016/j.procbio.2020.11.013http://www.journals.elsevier.com/process-biochemistry/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-30T01:26:30Zoai:repositorium.sdum.uminho.pt:1822/68442Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:48.224799Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution
title Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution
spellingShingle Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution
Alves, Rafael
Adaptive laboratory evolution
Hemicellulosic hydrolysate
Inhibtors
Tolerance
Clostridium saccharoperbutylacetonicum
Genomic analysis
Science & Technology
title_short Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution
title_full Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution
title_fullStr Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution
title_full_unstemmed Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution
title_sort Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution
author Alves, Rafael
author_facet Alves, Rafael
Zetty-Arenas, Ana M.
Demirci, H.
Dias, Oscar
Rocha, I.
Basso, Thiago O.
Freitas, Sindelia
author_role author
author2 Zetty-Arenas, Ana M.
Demirci, H.
Dias, Oscar
Rocha, I.
Basso, Thiago O.
Freitas, Sindelia
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Alves, Rafael
Zetty-Arenas, Ana M.
Demirci, H.
Dias, Oscar
Rocha, I.
Basso, Thiago O.
Freitas, Sindelia
dc.subject.por.fl_str_mv Adaptive laboratory evolution
Hemicellulosic hydrolysate
Inhibtors
Tolerance
Clostridium saccharoperbutylacetonicum
Genomic analysis
Science & Technology
topic Adaptive laboratory evolution
Hemicellulosic hydrolysate
Inhibtors
Tolerance
Clostridium saccharoperbutylacetonicum
Genomic analysis
Science & Technology
description Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.procbio.2020.11.013.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
2021-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/68442
url https://hdl.handle.net/1822/68442
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, Rafael; Zetty-Arenas, Ana M.; Demirci, H.; Dias, Oscar; Rocha, Isabel; Basso, Thiago O.; Freitas, Sindelia, Enhancing acetic acid and 5-hydroxymethyl furfural tolerance of C. saccharoperbutylacetonicum through adaptive laboratory evolution. Process Biochemistry, 101, 179-189, 2021
1359-5113
10.1016/j.procbio.2020.11.013
http://www.journals.elsevier.com/process-biochemistry/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132800177143808

Registros relacionados