Revealing the potential of squid chitosan-based structures for biomedical applications
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/24441 |
Resumo: | In recent years, much attention has been given to different marine organisms, namely as potential sources of valuable materials with a vast range of properties and characteristics. In this work, β-chitin was isolated from the endoskeleton of the giant squid Dosidicus gigas and further deacetylated to produce chitosan. Then, the squid chitosan was processed into membranes and scaffolds using solvent casting and freeze-drying, respectively, to assess their potential biomedical application. The developed membranes have shown to be stiffer and less hydrophobic than those obtained with commercial chitosan. On the other hand, the morphological characterization of the developed scaffolds, by SEM and micro-computed tomography, revealed that the matrices were formed with a lamellar structure. The findings also indicated that the treatment with ethanol prior to neutralization with sodium hydroxide caused the formation of larger pores and loss of some lamellar features. The in vitro cell culture study has shown that all chitosan scaffolds exhibited a non-cytotoxic effect over the mouse fibroblast-like cell line, L929 cells. Thus, chitosan produced from the endoskeletons of the giant squid Dosidicus gigas has proven to be a valuable alternative to existing commercial materials when considering its use as biomaterial. |
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Revealing the potential of squid chitosan-based structures for biomedical applicationsChitosanMarine-biomaterials.ScaffoldsSquid pensTissue engineeringβ-chitinScience & TechnologyIn recent years, much attention has been given to different marine organisms, namely as potential sources of valuable materials with a vast range of properties and characteristics. In this work, β-chitin was isolated from the endoskeleton of the giant squid Dosidicus gigas and further deacetylated to produce chitosan. Then, the squid chitosan was processed into membranes and scaffolds using solvent casting and freeze-drying, respectively, to assess their potential biomedical application. The developed membranes have shown to be stiffer and less hydrophobic than those obtained with commercial chitosan. On the other hand, the morphological characterization of the developed scaffolds, by SEM and micro-computed tomography, revealed that the matrices were formed with a lamellar structure. The findings also indicated that the treatment with ethanol prior to neutralization with sodium hydroxide caused the formation of larger pores and loss of some lamellar features. The in vitro cell culture study has shown that all chitosan scaffolds exhibited a non-cytotoxic effect over the mouse fibroblast-like cell line, L929 cells. Thus, chitosan produced from the endoskeletons of the giant squid Dosidicus gigas has proven to be a valuable alternative to existing commercial materials when considering its use as biomaterial.This work was partially funded by FEDER through INTERREG III A Project Proteus and POCTEP Project IBEROMARE. The Portuguese Foundation for Science and Technology is gratefully acknowledged for post-doctoral grants of THS, JMO and SSS. The authors would also like to acknowledge to Dr Julio Maroto from the Fundacion CETMAR and Roi Vilela from PESCANOVA S.A, Spain, for the kind offer of squid pens and to Dr Ramon Novoa, Professor Ricardo Riguera and Professor Mariana Landin from the University of Santiago of Compostela for the SEC-MALLS measurements.IOP PublishingUniversidade do MinhoReys, L. L.Silva, Simone SantosOliveira, Joaquim M.Caridade, S. G.Mano, J. F.Silva, Tiago H.Reis, R. L.2013-052013-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/24441eng1748-605X10.1088/1748-6041/8/4/04500223715133http://iopscience.iop.org/1748-605X/8/4/045002/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:20Zoai:repositorium.sdum.uminho.pt:1822/24441Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:30:00.227177Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Revealing the potential of squid chitosan-based structures for biomedical applications |
title |
Revealing the potential of squid chitosan-based structures for biomedical applications |
spellingShingle |
Revealing the potential of squid chitosan-based structures for biomedical applications Reys, L. L. Chitosan Marine-biomaterials. Scaffolds Squid pens Tissue engineering β-chitin Science & Technology |
title_short |
Revealing the potential of squid chitosan-based structures for biomedical applications |
title_full |
Revealing the potential of squid chitosan-based structures for biomedical applications |
title_fullStr |
Revealing the potential of squid chitosan-based structures for biomedical applications |
title_full_unstemmed |
Revealing the potential of squid chitosan-based structures for biomedical applications |
title_sort |
Revealing the potential of squid chitosan-based structures for biomedical applications |
author |
Reys, L. L. |
author_facet |
Reys, L. L. Silva, Simone Santos Oliveira, Joaquim M. Caridade, S. G. Mano, J. F. Silva, Tiago H. Reis, R. L. |
author_role |
author |
author2 |
Silva, Simone Santos Oliveira, Joaquim M. Caridade, S. G. Mano, J. F. Silva, Tiago H. Reis, R. L. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Reys, L. L. Silva, Simone Santos Oliveira, Joaquim M. Caridade, S. G. Mano, J. F. Silva, Tiago H. Reis, R. L. |
dc.subject.por.fl_str_mv |
Chitosan Marine-biomaterials. Scaffolds Squid pens Tissue engineering β-chitin Science & Technology |
topic |
Chitosan Marine-biomaterials. Scaffolds Squid pens Tissue engineering β-chitin Science & Technology |
description |
In recent years, much attention has been given to different marine organisms, namely as potential sources of valuable materials with a vast range of properties and characteristics. In this work, β-chitin was isolated from the endoskeleton of the giant squid Dosidicus gigas and further deacetylated to produce chitosan. Then, the squid chitosan was processed into membranes and scaffolds using solvent casting and freeze-drying, respectively, to assess their potential biomedical application. The developed membranes have shown to be stiffer and less hydrophobic than those obtained with commercial chitosan. On the other hand, the morphological characterization of the developed scaffolds, by SEM and micro-computed tomography, revealed that the matrices were formed with a lamellar structure. The findings also indicated that the treatment with ethanol prior to neutralization with sodium hydroxide caused the formation of larger pores and loss of some lamellar features. The in vitro cell culture study has shown that all chitosan scaffolds exhibited a non-cytotoxic effect over the mouse fibroblast-like cell line, L929 cells. Thus, chitosan produced from the endoskeletons of the giant squid Dosidicus gigas has proven to be a valuable alternative to existing commercial materials when considering its use as biomaterial. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-05 2013-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/24441 |
url |
http://hdl.handle.net/1822/24441 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1748-605X 10.1088/1748-6041/8/4/045002 23715133 http://iopscience.iop.org/1748-605X/8/4/045002/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IOP Publishing |
publisher.none.fl_str_mv |
IOP Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132801472135168 |