Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/4981 |
Resumo: | Sulfate reducing bacteria (SRB) are widely used for attenuating heavy metal pollution by means of sulfide generation. Due to their low metal tolerance, several SRB species depend on associated bacteria in mixed cultures to cope with metal-induced stress. Yet the identity of the SRB protecting bacteria is largely unknown. We aimed to identify these associated bacteria and their potential role in two highly metal-resistant mixed SRB cultures by comparing bacterial community composition and SRB activity between these cultures and two sensitive ones. The SRB composition in the resistant and sensitive consortia was similar. However, whereas the SRB in the sensitive cultures were strongly inhibited by a mixture of copper, zinc, and iron, no influence of these metals was detected on SRB growth and activity in the resistant cultures. In the latter, a Gram-positive population mostly assigned to Clostridium spp.initiated heavy metal bioremoval based on sulfide generation from components of the medium (mainly sulfite) but not from sulfate. After metal levels were lowered by the Clostridium spp. populations, SRB started sulfate reduction and raised the pH of the medium. The combination of sulfite reducing Clostridium spp. with SRB may improve green technologies for removal of heavy metals. |
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Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic CulturesSulfate reducing bacteria (SRB) are widely used for attenuating heavy metal pollution by means of sulfide generation. Due to their low metal tolerance, several SRB species depend on associated bacteria in mixed cultures to cope with metal-induced stress. Yet the identity of the SRB protecting bacteria is largely unknown. We aimed to identify these associated bacteria and their potential role in two highly metal-resistant mixed SRB cultures by comparing bacterial community composition and SRB activity between these cultures and two sensitive ones. The SRB composition in the resistant and sensitive consortia was similar. However, whereas the SRB in the sensitive cultures were strongly inhibited by a mixture of copper, zinc, and iron, no influence of these metals was detected on SRB growth and activity in the resistant cultures. In the latter, a Gram-positive population mostly assigned to Clostridium spp.initiated heavy metal bioremoval based on sulfide generation from components of the medium (mainly sulfite) but not from sulfate. After metal levels were lowered by the Clostridium spp. populations, SRB started sulfate reduction and raised the pH of the medium. The combination of sulfite reducing Clostridium spp. with SRB may improve green technologies for removal of heavy metals.ACS PublicationsSapientiaAlexandrino, MariaCosta, RodrigoCanario, Adelino V. M.Costa, Maria Clara2015-03-18T01:30:06Z2014-03-182014-03-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/4981engMaria Alexandrino, Rodrigo Costa, Adelino V. M. Canário, and Maria C. Costa. (2014). Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures, Environmental Science and Technology, 3378-3385, dx.doi.org/10.1021/es40520440013-936XAUT: ACA00258; MCO01181;http://dx.doi.org/10.1021/es4052044info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:16:09Zoai:sapientia.ualg.pt:10400.1/4981Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:58:10.004982Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures |
title |
Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures |
spellingShingle |
Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures Alexandrino, Maria |
title_short |
Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures |
title_full |
Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures |
title_fullStr |
Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures |
title_full_unstemmed |
Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures |
title_sort |
Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures |
author |
Alexandrino, Maria |
author_facet |
Alexandrino, Maria Costa, Rodrigo Canario, Adelino V. M. Costa, Maria Clara |
author_role |
author |
author2 |
Costa, Rodrigo Canario, Adelino V. M. Costa, Maria Clara |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Alexandrino, Maria Costa, Rodrigo Canario, Adelino V. M. Costa, Maria Clara |
description |
Sulfate reducing bacteria (SRB) are widely used for attenuating heavy metal pollution by means of sulfide generation. Due to their low metal tolerance, several SRB species depend on associated bacteria in mixed cultures to cope with metal-induced stress. Yet the identity of the SRB protecting bacteria is largely unknown. We aimed to identify these associated bacteria and their potential role in two highly metal-resistant mixed SRB cultures by comparing bacterial community composition and SRB activity between these cultures and two sensitive ones. The SRB composition in the resistant and sensitive consortia was similar. However, whereas the SRB in the sensitive cultures were strongly inhibited by a mixture of copper, zinc, and iron, no influence of these metals was detected on SRB growth and activity in the resistant cultures. In the latter, a Gram-positive population mostly assigned to Clostridium spp.initiated heavy metal bioremoval based on sulfide generation from components of the medium (mainly sulfite) but not from sulfate. After metal levels were lowered by the Clostridium spp. populations, SRB started sulfate reduction and raised the pH of the medium. The combination of sulfite reducing Clostridium spp. with SRB may improve green technologies for removal of heavy metals. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-18 2014-03-18T00:00:00Z 2015-03-18T01:30:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/4981 |
url |
http://hdl.handle.net/10400.1/4981 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Maria Alexandrino, Rodrigo Costa, Adelino V. M. Canário, and Maria C. Costa. (2014). Clostridia Initiate Heavy Metal Bioremoval in Mixed Sulfidogenic Cultures, Environmental Science and Technology, 3378-3385, dx.doi.org/10.1021/es4052044 0013-936X AUT: ACA00258; MCO01181; http://dx.doi.org/10.1021/es4052044 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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ACS Publications |
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ACS Publications |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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