A new clonal propagation protocol develops quality root systems in chestnut

Detalhes bibliográficos
Autor(a) principal: Fernandes, Patrícia
Data de Publicação: 2020
Outros Autores: Tedesco, Sara, Silva, Inês Vieira da, Santos, Carmen, Machado, Helena, Costa, Rita Lourenço
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/20342
Resumo: Technical Note
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spelling A new clonal propagation protocol develops quality root systems in chestnutaxillary shoot proliferationCastanea hybridsrootingrootstockstissue cultureTechnical NoteThere is, at the present time, a great demand for chestnut rootstocks with improved resistance to Phytophthora cinnamomi Rands in the nurseries. New genotypes are emerging from European chestnut breeding programs and the production of thriving plants to restore old orchards with low yields due to a high incidence of diseases, namely root rot, is necessary. Micropropagation is a useful technique for clonal propagation. Nevertheless, in vitro culture propagation is genotype-dependent. Consequently, the existing protocols may demonstrate poor reproducibility and low e cacy. Thus, the need to contribute to the development of new micropropagation protocols suitable for large production of emerging genotypes. As a contribution to fill this gap, a three-step protocol was developed by using new combinations of Murashige & Skoog, Woody Plant, and adapted modified Melin-Norkrans media in di erent stages of the propagation process. About 90% of shoots were rooted, and after three months of acclimatization, 85% of these plants survived and were capable of continuous growth in the field. Currently, this protocol is being used in the production of several hybrid genotypes (with improved resistance to P. cinnamomi), selected from our ongoing breeding program and also in Castanea sativa Mill. and Castanea crenata Siebold and Zucc. speciesMDPIRepositório da Universidade de LisboaFernandes, PatríciaTedesco, SaraSilva, Inês Vieira daSantos, CarmenMachado, HelenaCosta, Rita Lourenço2020-09-17T14:04:48Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/20342engForests 2020, 11, 82610.3390/f11080826info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:49:47ZPortal AgregadorONG
dc.title.none.fl_str_mv A new clonal propagation protocol develops quality root systems in chestnut
title A new clonal propagation protocol develops quality root systems in chestnut
spellingShingle A new clonal propagation protocol develops quality root systems in chestnut
Fernandes, Patrícia
axillary shoot proliferation
Castanea hybrids
rooting
rootstocks
tissue culture
title_short A new clonal propagation protocol develops quality root systems in chestnut
title_full A new clonal propagation protocol develops quality root systems in chestnut
title_fullStr A new clonal propagation protocol develops quality root systems in chestnut
title_full_unstemmed A new clonal propagation protocol develops quality root systems in chestnut
title_sort A new clonal propagation protocol develops quality root systems in chestnut
author Fernandes, Patrícia
author_facet Fernandes, Patrícia
Tedesco, Sara
Silva, Inês Vieira da
Santos, Carmen
Machado, Helena
Costa, Rita Lourenço
author_role author
author2 Tedesco, Sara
Silva, Inês Vieira da
Santos, Carmen
Machado, Helena
Costa, Rita Lourenço
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Fernandes, Patrícia
Tedesco, Sara
Silva, Inês Vieira da
Santos, Carmen
Machado, Helena
Costa, Rita Lourenço
dc.subject.por.fl_str_mv axillary shoot proliferation
Castanea hybrids
rooting
rootstocks
tissue culture
topic axillary shoot proliferation
Castanea hybrids
rooting
rootstocks
tissue culture
description Technical Note
publishDate 2020
dc.date.none.fl_str_mv 2020-09-17T14:04:48Z
2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/20342
url http://hdl.handle.net/10400.5/20342
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Forests 2020, 11, 826
10.3390/f11080826
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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