Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves

Detalhes bibliográficos
Autor(a) principal: Alves, Patrícia
Data de Publicação: 2014
Outros Autores: Cardoso, Rute, Correia, Tiago R., Antunes, Bernardo Paiva, Correia, Ilídio Joaquim Sobreira, Ferreira, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/4652
Resumo: Prosthetic cardiac valves implantation is a common procedure used to treat heart valve diseases. Although there are different prostheses already available in the market (either mechanical or bioprosthetic), their use presents several problems, specifically concerning thrombogenicity and structural failure. Recently, some progresses have been achieved in developing heart valves based on synthetic materials with special emphasis in polymers. Among them, polyurethanes are one of the most commonly used for the production of these devices. Herein, Elastollan®1180A50, a thermoplastic polyurethane (TPU), was used to formulate films whose surfaces were modified by grafting 2-hydroxyethylmethacrylate (HEMA) either by ultra-violet (UV) or by plasma treatment. All films were analyzed before and after grafting. X-ray photoelectron spectroscopy (XPS) measurements were used to evaluate TPU surfaces functionalization. HEMA grafting was confirmed by the increase of the hydroxyl (OH) groups’ concentration at the surface of the films. Atomic force microscopy (AFM) analysis was done to evaluate the surface topography of the biomaterials. Results showed that the roughness of the surface decreased when HEMA was grafted, especially for plasma treated samples. After grafting the films’ hydrophilicity was improved, as well as the polar component of the surface energy, by 15–30%. Hydrophobic recovery studies using milli Q water or PBS were also performed to characterize the stability of the modified surface, showing that the films maintained their surface properties along time. Furthermore, blood-contact tests were performed to evaluate haemolytic and thrombogenic potential. The results obtained for HEMA grafted surfaces, using plasma treatment, confirmed biomaterials biocompatibility and low thrombogenicity. Finally, the cytotoxicity and antibacterial activity of the materials was assessed through in vitro assays for both modified films. The obtained results showed enhanced bactericidal activity, especially for the films modified with plasma.
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spelling Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valvesHeart valvesPlasma and UV activationPolyurethanesSurface modificationBiocompatibilityProsthetic cardiac valves implantation is a common procedure used to treat heart valve diseases. Although there are different prostheses already available in the market (either mechanical or bioprosthetic), their use presents several problems, specifically concerning thrombogenicity and structural failure. Recently, some progresses have been achieved in developing heart valves based on synthetic materials with special emphasis in polymers. Among them, polyurethanes are one of the most commonly used for the production of these devices. Herein, Elastollan®1180A50, a thermoplastic polyurethane (TPU), was used to formulate films whose surfaces were modified by grafting 2-hydroxyethylmethacrylate (HEMA) either by ultra-violet (UV) or by plasma treatment. All films were analyzed before and after grafting. X-ray photoelectron spectroscopy (XPS) measurements were used to evaluate TPU surfaces functionalization. HEMA grafting was confirmed by the increase of the hydroxyl (OH) groups’ concentration at the surface of the films. Atomic force microscopy (AFM) analysis was done to evaluate the surface topography of the biomaterials. Results showed that the roughness of the surface decreased when HEMA was grafted, especially for plasma treated samples. After grafting the films’ hydrophilicity was improved, as well as the polar component of the surface energy, by 15–30%. Hydrophobic recovery studies using milli Q water or PBS were also performed to characterize the stability of the modified surface, showing that the films maintained their surface properties along time. Furthermore, blood-contact tests were performed to evaluate haemolytic and thrombogenic potential. The results obtained for HEMA grafted surfaces, using plasma treatment, confirmed biomaterials biocompatibility and low thrombogenicity. Finally, the cytotoxicity and antibacterial activity of the materials was assessed through in vitro assays for both modified films. The obtained results showed enhanced bactericidal activity, especially for the films modified with plasma.ElsevieruBibliorumAlves, PatríciaCardoso, RuteCorreia, Tiago R.Antunes, Bernardo PaivaCorreia, Ilídio Joaquim SobreiraFerreira, Paula2018-03-20T11:07:44Z2014-01-012014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4652engAlves, P., Cardoso, R., Correia, T.R., Antunes, B.P., Correia, I.J. e Ferreira, P. (2014) “Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves”, Colloids and Surfaces B Biointerfaces, Vol. 113, pp. 25-3210.1016/j.colsurfb.2013.08.039metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-07T02:30:47Zoai:ubibliorum.ubi.pt:10400.6/4652Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:42.716649Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves
title Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves
spellingShingle Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves
Alves, Patrícia
Heart valves
Plasma and UV activation
Polyurethanes
Surface modification
Biocompatibility
title_short Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves
title_full Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves
title_fullStr Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves
title_full_unstemmed Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves
title_sort Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves
author Alves, Patrícia
author_facet Alves, Patrícia
Cardoso, Rute
Correia, Tiago R.
Antunes, Bernardo Paiva
Correia, Ilídio Joaquim Sobreira
Ferreira, Paula
author_role author
author2 Cardoso, Rute
Correia, Tiago R.
Antunes, Bernardo Paiva
Correia, Ilídio Joaquim Sobreira
Ferreira, Paula
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Alves, Patrícia
Cardoso, Rute
Correia, Tiago R.
Antunes, Bernardo Paiva
Correia, Ilídio Joaquim Sobreira
Ferreira, Paula
dc.subject.por.fl_str_mv Heart valves
Plasma and UV activation
Polyurethanes
Surface modification
Biocompatibility
topic Heart valves
Plasma and UV activation
Polyurethanes
Surface modification
Biocompatibility
description Prosthetic cardiac valves implantation is a common procedure used to treat heart valve diseases. Although there are different prostheses already available in the market (either mechanical or bioprosthetic), their use presents several problems, specifically concerning thrombogenicity and structural failure. Recently, some progresses have been achieved in developing heart valves based on synthetic materials with special emphasis in polymers. Among them, polyurethanes are one of the most commonly used for the production of these devices. Herein, Elastollan®1180A50, a thermoplastic polyurethane (TPU), was used to formulate films whose surfaces were modified by grafting 2-hydroxyethylmethacrylate (HEMA) either by ultra-violet (UV) or by plasma treatment. All films were analyzed before and after grafting. X-ray photoelectron spectroscopy (XPS) measurements were used to evaluate TPU surfaces functionalization. HEMA grafting was confirmed by the increase of the hydroxyl (OH) groups’ concentration at the surface of the films. Atomic force microscopy (AFM) analysis was done to evaluate the surface topography of the biomaterials. Results showed that the roughness of the surface decreased when HEMA was grafted, especially for plasma treated samples. After grafting the films’ hydrophilicity was improved, as well as the polar component of the surface energy, by 15–30%. Hydrophobic recovery studies using milli Q water or PBS were also performed to characterize the stability of the modified surface, showing that the films maintained their surface properties along time. Furthermore, blood-contact tests were performed to evaluate haemolytic and thrombogenic potential. The results obtained for HEMA grafted surfaces, using plasma treatment, confirmed biomaterials biocompatibility and low thrombogenicity. Finally, the cytotoxicity and antibacterial activity of the materials was assessed through in vitro assays for both modified films. The obtained results showed enhanced bactericidal activity, especially for the films modified with plasma.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2014-01-01T00:00:00Z
2018-03-20T11:07:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/4652
url http://hdl.handle.net/10400.6/4652
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, P., Cardoso, R., Correia, T.R., Antunes, B.P., Correia, I.J. e Ferreira, P. (2014) “Surface modification of polyurethane films by plasma and ultraviolet light to improve haemocompatibility for artificial heart valves”, Colloids and Surfaces B Biointerfaces, Vol. 113, pp. 25-32
10.1016/j.colsurfb.2013.08.039
dc.rights.driver.fl_str_mv metadata only access
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rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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