Maximization of fructose esters synthesis by response surface methodology

Detalhes bibliográficos
Autor(a) principal: Neta, Nair S.
Data de Publicação: 2011
Outros Autores: Peres, António M., Teixeira, José, Rodrigues, Lígia R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/6406
Resumo: Enzymatic synthesis of fructose fatty acid ester was performed in organic solvent media, using a purified lipase from Candida antartica B immobilized in acrylic resin. Response surface methodology with a central composite rotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature and reaction time were found to be the most significant parameters. The optimum operational conditions for maximizing the synthesis of fructose esters were 57.18C, 100 rpm and 37.8 h. The model was validated in the identified optimal conditions to check its adequacy and accuracy, and an experimental esterification percentage of 88.4% (+/-0.3%) was obtained. These results showed that an improvement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions.
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spelling Maximization of fructose esters synthesis by response surface methodologyCandida antartica BFructose estersResponse surface methodologyEnzymatic synthesis of fructose fatty acid ester was performed in organic solvent media, using a purified lipase from Candida antartica B immobilized in acrylic resin. Response surface methodology with a central composite rotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature and reaction time were found to be the most significant parameters. The optimum operational conditions for maximizing the synthesis of fructose esters were 57.18C, 100 rpm and 37.8 h. The model was validated in the identified optimal conditions to check its adequacy and accuracy, and an experimental esterification percentage of 88.4% (+/-0.3%) was obtained. These results showed that an improvement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions.The authors acknowledge the AlBan Programme (European Union Programme of High Level Scholarships for Latin America) for financial support of N. Lopes through the research grant E07D401544BRElsevierBiblioteca Digital do IPBNeta, Nair S.Peres, António M.Teixeira, JoséRodrigues, Lígia R.2011-12-06T14:14:32Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/6406engNeta, Nair S.; Peres, António M.; Teixeira, José A,; Rodrigues, Lígia R. (2011). Maximization of fructose esters synthesis by response surface methodology. New Biotechnology. ISSN 1871-6784. 28:4, p. 349-3551871-678410.1016/j.nbt.2011.02.007info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:16:09Zoai:bibliotecadigital.ipb.pt:10198/6406Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:58:27.176393Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Maximization of fructose esters synthesis by response surface methodology
title Maximization of fructose esters synthesis by response surface methodology
spellingShingle Maximization of fructose esters synthesis by response surface methodology
Neta, Nair S.
Candida antartica B
Fructose esters
Response surface methodology
title_short Maximization of fructose esters synthesis by response surface methodology
title_full Maximization of fructose esters synthesis by response surface methodology
title_fullStr Maximization of fructose esters synthesis by response surface methodology
title_full_unstemmed Maximization of fructose esters synthesis by response surface methodology
title_sort Maximization of fructose esters synthesis by response surface methodology
author Neta, Nair S.
author_facet Neta, Nair S.
Peres, António M.
Teixeira, José
Rodrigues, Lígia R.
author_role author
author2 Peres, António M.
Teixeira, José
Rodrigues, Lígia R.
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Neta, Nair S.
Peres, António M.
Teixeira, José
Rodrigues, Lígia R.
dc.subject.por.fl_str_mv Candida antartica B
Fructose esters
Response surface methodology
topic Candida antartica B
Fructose esters
Response surface methodology
description Enzymatic synthesis of fructose fatty acid ester was performed in organic solvent media, using a purified lipase from Candida antartica B immobilized in acrylic resin. Response surface methodology with a central composite rotatable design based on five levels was implemented to optimize three experimental operating conditions (temperature, agitation and reaction time). A statistical significant cubic model was established. Temperature and reaction time were found to be the most significant parameters. The optimum operational conditions for maximizing the synthesis of fructose esters were 57.18C, 100 rpm and 37.8 h. The model was validated in the identified optimal conditions to check its adequacy and accuracy, and an experimental esterification percentage of 88.4% (+/-0.3%) was obtained. These results showed that an improvement of the enzymatic synthesis of fructose esters was obtained under the optimized conditions.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-06T14:14:32Z
2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/6406
url http://hdl.handle.net/10198/6406
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Neta, Nair S.; Peres, António M.; Teixeira, José A,; Rodrigues, Lígia R. (2011). Maximization of fructose esters synthesis by response surface methodology. New Biotechnology. ISSN 1871-6784. 28:4, p. 349-355
1871-6784
10.1016/j.nbt.2011.02.007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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