Identification of proteins with potential osteogenic activity present in the water-soluble matrix proteins from crassostrea gigas nacre using a proteomic approach

• Autor(a) principal: Oliveira, Daniel
• Data de Publicação: 2012
• Outros Autores: Silva, Tome S., Cordeiro, Odete D., Cavaco, Sofia I., Simes, Dina
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.1/12065
• topic: Oyster pinctada-fucata; Tenascin-C; Organic matrix; Cathepsin-K; Cysteine proteinases; Bone-resorption; Cystatin-C; In-Vivo; Pacific oyster; Stefin-A

Nacre, when implanted in vivo in bones of dogs, sheep, mice, and humans, induces a biological response that includes integration and osteogenic activity on the host tissue that seems to be activated by a set of proteins present in the nacre water-soluble matrix (WSM). We describe here an experimental approach that can accurately identify the proteins present in the WSM of shell mollusk nacre. Four proteins (three gigasin-2 isoforms and a cystatin A2) were for the first time identified in WSM of Crassostrea gigas nacre using 2DE and LC-MS/MS for protein identification. These proteins are thought to be involved in bone remodeling processes and could be responsible for the biocompatibility shown between bone and nacre grafts. These results represent a contribution to the study of shell biomineralization process and opens new perspectives for the development of new nacre biomaterials for orthopedic applications.