Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line

Detalhes bibliográficos
Autor(a) principal: Cruz, M. Teresa
Data de Publicação: 2004
Outros Autores: Gonçalo, Margarida, Figueiredo, Américo, Carvalho, Arsélio P., Duarte, Carlos B., Lopes, M. Celeste
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/11804
https://doi.org/10.1016/s0378-4274(03)90098-1
Resumo: Nuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO4) and increases the expression of the iNOS protein, as determined by immunofluorescence and Western blot analysis. The sensitizer NiSO4 increased cytoplasmic iNOS expression by 31.9+10.3% and nitrite production, as assayed by the Griess reaction, by 27.6+9.5%. Electrophoretic mobility shift assay (EMSA), showed that 30min of FSDC exposure to NiSO4 activates the transcription factor NF-kB by 58.2+7.0% and 2 h of FSDC exposure to NiSO4 activates the transcription factor AP-1 by 26.0+1.4%. Together, these results indicate that NiSO4 activates the NF-kB and AP-1 pathways and induces iNOS expression in skin dendritic cells.
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spelling Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell lineNOiNOSSkin dendritic cellContact sensitizerNickel sulfateNF-kBAP-1Nuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO4) and increases the expression of the iNOS protein, as determined by immunofluorescence and Western blot analysis. The sensitizer NiSO4 increased cytoplasmic iNOS expression by 31.9+10.3% and nitrite production, as assayed by the Griess reaction, by 27.6+9.5%. Electrophoretic mobility shift assay (EMSA), showed that 30min of FSDC exposure to NiSO4 activates the transcription factor NF-kB by 58.2+7.0% and 2 h of FSDC exposure to NiSO4 activates the transcription factor AP-1 by 26.0+1.4%. Together, these results indicate that NiSO4 activates the NF-kB and AP-1 pathways and induces iNOS expression in skin dendritic cells.Blackwell Munksgaard2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/11804http://hdl.handle.net/10316/11804https://doi.org/10.1016/s0378-4274(03)90098-1engExperimental Dermatology. 13 (2004) 18–260906-6705Cruz, M. TeresaGonçalo, MargaridaFigueiredo, AméricoCarvalho, Arsélio P.Duarte, Carlos B.Lopes, M. Celesteinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:59:50Zoai:estudogeral.uc.pt:10316/11804Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:25.371979Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
title Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
spellingShingle Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
Cruz, M. Teresa
NO
iNOS
Skin dendritic cell
Contact sensitizer
Nickel sulfate
NF-kB
AP-1
title_short Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
title_full Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
title_fullStr Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
title_full_unstemmed Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
title_sort Contact sensitizer nickel sulfate activates the transcription factors NF-kB and AP-1 and increases the expression of nitric oxide synthase in a skin dendritic cell line
author Cruz, M. Teresa
author_facet Cruz, M. Teresa
Gonçalo, Margarida
Figueiredo, Américo
Carvalho, Arsélio P.
Duarte, Carlos B.
Lopes, M. Celeste
author_role author
author2 Gonçalo, Margarida
Figueiredo, Américo
Carvalho, Arsélio P.
Duarte, Carlos B.
Lopes, M. Celeste
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cruz, M. Teresa
Gonçalo, Margarida
Figueiredo, Américo
Carvalho, Arsélio P.
Duarte, Carlos B.
Lopes, M. Celeste
dc.subject.por.fl_str_mv NO
iNOS
Skin dendritic cell
Contact sensitizer
Nickel sulfate
NF-kB
AP-1
topic NO
iNOS
Skin dendritic cell
Contact sensitizer
Nickel sulfate
NF-kB
AP-1
description Nuclear factor kappa B (NF-kB) and activating protein-1 (AP-1) transcription factors are ubiquitously expressed signaling molecules known to regulate the transcription of a large number of genes involved in immune responses, namely the inducible isoform of nitric oxide synthase (iNOS). In this study, we demonstrate that a fetal skin-derived dendritic cell line (FSDC) produces nitric oxide (NO) in response to the contact sensitizer nickel sulfate (NiSO4) and increases the expression of the iNOS protein, as determined by immunofluorescence and Western blot analysis. The sensitizer NiSO4 increased cytoplasmic iNOS expression by 31.9+10.3% and nitrite production, as assayed by the Griess reaction, by 27.6+9.5%. Electrophoretic mobility shift assay (EMSA), showed that 30min of FSDC exposure to NiSO4 activates the transcription factor NF-kB by 58.2+7.0% and 2 h of FSDC exposure to NiSO4 activates the transcription factor AP-1 by 26.0+1.4%. Together, these results indicate that NiSO4 activates the NF-kB and AP-1 pathways and induces iNOS expression in skin dendritic cells.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/11804
http://hdl.handle.net/10316/11804
https://doi.org/10.1016/s0378-4274(03)90098-1
url http://hdl.handle.net/10316/11804
https://doi.org/10.1016/s0378-4274(03)90098-1
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Experimental Dermatology. 13 (2004) 18–26
0906-6705
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Munksgaard
publisher.none.fl_str_mv Blackwell Munksgaard
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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