Microglial clearance function: dependence on phenotypes

Detalhes bibliográficos
Autor(a) principal: Oliveira, Ana Filipa Martins
Data de Publicação: 2011
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/6715
Resumo: Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
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spelling Microglial clearance function: dependence on phenotypesUnconjugated bilirubinMicroglia morphologyCell migrationPhagocytosisNuclear factor-kappaBGlycoursodeoxycholic acidDissertação para obtenção do Grau de Mestre em Genética Molecular e BiomedicinaMicroglia are active sensors of the brain and respond promptly to even minor disturbance in their microenvironment. A feature of this response is the accumulation of these cells at the site of lesion. Neonatal jaundice is a common condition of the newborn and may determine injury to neurons and glial cells, such as microglia, when levels of unconjugated bilirubin (UCB) are excessive. With the objective to evaluate whether microglia have a protective or deleterious role, we decided to assess, using the Boyden chamber, the chemotactic effect of free unbound UCB (fUCB), as well as the migration ability of UCB-treated microglia in the absence or in the presence of chemotatic compounds, such as ATP and S100B. Also, we intended to evaluate the effect of glycoursodeoxycholic acid (GUDCA) as a modulator. To characterize our usual model of microglia isolation, phenotypic evaluation of cultures with different days in vitro (DIV) was performed by estimating cell morphology, nuclear factor-kappaB (NF-κB) activation and phagocytic ability. We observed that fUCB did not act as a chemotactic compound for microglia and that cells treated with UCB showed decreased migration ability. Co-incubation with GUDCA prevented this effect and enhanced microglia migration. However, reduced effects were observed in the presence of ATP and abolished when using S100B. Isolated microglia with 2 DIV showed features of activation, but presentedramified morphology of the “resting” state, less NF-κB activation and increased phagocytosis at 13 DIV. Data indicate that microoglia exposure to UCB leads to a reduced migration ability and that co-incubation with GUDCA prevents this deleterious effect, resulting in an increased migration. Characterization of microglia phenotypes, along the time in culture, point to 13 DIV cells as the most suitable for studies intended to evaluate microglia reactivity to UCB, and probably to other stimuli.Faculdade de Ciências e TecnologiaBrites, DoraJesus, AnaRUNOliveira, Ana Filipa Martins2012-01-12T15:11:37Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/6715enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:37:56Zoai:run.unl.pt:10362/6715Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:16:57.828163Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microglial clearance function: dependence on phenotypes
title Microglial clearance function: dependence on phenotypes
spellingShingle Microglial clearance function: dependence on phenotypes
Oliveira, Ana Filipa Martins
Unconjugated bilirubin
Microglia morphology
Cell migration
Phagocytosis
Nuclear factor-kappaB
Glycoursodeoxycholic acid
title_short Microglial clearance function: dependence on phenotypes
title_full Microglial clearance function: dependence on phenotypes
title_fullStr Microglial clearance function: dependence on phenotypes
title_full_unstemmed Microglial clearance function: dependence on phenotypes
title_sort Microglial clearance function: dependence on phenotypes
author Oliveira, Ana Filipa Martins
author_facet Oliveira, Ana Filipa Martins
author_role author
dc.contributor.none.fl_str_mv Brites, Dora
Jesus, Ana
RUN
dc.contributor.author.fl_str_mv Oliveira, Ana Filipa Martins
dc.subject.por.fl_str_mv Unconjugated bilirubin
Microglia morphology
Cell migration
Phagocytosis
Nuclear factor-kappaB
Glycoursodeoxycholic acid
topic Unconjugated bilirubin
Microglia morphology
Cell migration
Phagocytosis
Nuclear factor-kappaB
Glycoursodeoxycholic acid
description Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2012-01-12T15:11:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/6715
url http://hdl.handle.net/10362/6715
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137818597916672

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