Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed

Detalhes bibliográficos
Autor(a) principal: Kumar, Praveen Kumar
Data de Publicação: 2010
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/10738
Resumo: In this work, clean up strategy is optimized for an analytical method based on liquid chromatography with UV detection for determination of eight sulfonamides (sulfachloropyridazine, sulfadiazine, sulfadimidine, sulfadoxine, sulfamethoxypyridazine, sulfaquinoxaline, sulfamethoxazole and sulfadimethoxine) in pig feed. Solid Phase Extraction is used for clean up and the performance of different sorbents - C 18, Oasis HLB, Plexa and Plexa PCX is assessed by their clean up efficiency and recovery of analytes. Oasis HLB and Plexa PCX sorbents provide better clean up and recovery values than C18 and Plexa. A spiked feed of 2 mg/kg is used to assess mean recoveries and precision in terms of repeatability, r and within-lab reproducibility, R for the whole method with Oasis HLB and Plexa PCX. Recoveries using Oasis HLB ranges from 51 to 78%, with RSDr from 5 to 15% and RSDR from 5 to 18%. Recoveries using Plexa PCX ranges from 64 to 85% with RSDr from 4 to 11% and RSDR from 7 to 17%..
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spelling Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feedSulfonamidesAnimal feedDomínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e TecnologiasIn this work, clean up strategy is optimized for an analytical method based on liquid chromatography with UV detection for determination of eight sulfonamides (sulfachloropyridazine, sulfadiazine, sulfadimidine, sulfadoxine, sulfamethoxypyridazine, sulfaquinoxaline, sulfamethoxazole and sulfadimethoxine) in pig feed. Solid Phase Extraction is used for clean up and the performance of different sorbents - C 18, Oasis HLB, Plexa and Plexa PCX is assessed by their clean up efficiency and recovery of analytes. Oasis HLB and Plexa PCX sorbents provide better clean up and recovery values than C18 and Plexa. A spiked feed of 2 mg/kg is used to assess mean recoveries and precision in terms of repeatability, r and within-lab reproducibility, R for the whole method with Oasis HLB and Plexa PCX. Recoveries using Oasis HLB ranges from 51 to 78%, with RSDr from 5 to 15% and RSDR from 5 to 18%. Recoveries using Plexa PCX ranges from 64 to 85% with RSDr from 4 to 11% and RSDR from 7 to 17%..Companyó, RamonCavaco, Isabel Maria Palma Antunes ,SapientiaKumar, Praveen Kumar2018-07-02T09:40:09Z2010-02-1820102010-02-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.1/10738TID:202467791enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:22:24Zoai:sapientia.ualg.pt:10400.1/10738Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:02:21.126945Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed
title Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed
spellingShingle Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed
Kumar, Praveen Kumar
Sulfonamides
Animal feed
Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias
title_short Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed
title_full Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed
title_fullStr Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed
title_full_unstemmed Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed
title_sort Optimization of an SPE clean-up approach for the analysis of sulfonamides in animal feed
author Kumar, Praveen Kumar
author_facet Kumar, Praveen Kumar
author_role author
dc.contributor.none.fl_str_mv Companyó, Ramon
Cavaco, Isabel Maria Palma Antunes ,
Sapientia
dc.contributor.author.fl_str_mv Kumar, Praveen Kumar
dc.subject.por.fl_str_mv Sulfonamides
Animal feed
Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias
topic Sulfonamides
Animal feed
Domínio/Área Científica::Engenharia e Tecnologia::Outras Engenharias e Tecnologias
description In this work, clean up strategy is optimized for an analytical method based on liquid chromatography with UV detection for determination of eight sulfonamides (sulfachloropyridazine, sulfadiazine, sulfadimidine, sulfadoxine, sulfamethoxypyridazine, sulfaquinoxaline, sulfamethoxazole and sulfadimethoxine) in pig feed. Solid Phase Extraction is used for clean up and the performance of different sorbents - C 18, Oasis HLB, Plexa and Plexa PCX is assessed by their clean up efficiency and recovery of analytes. Oasis HLB and Plexa PCX sorbents provide better clean up and recovery values than C18 and Plexa. A spiked feed of 2 mg/kg is used to assess mean recoveries and precision in terms of repeatability, r and within-lab reproducibility, R for the whole method with Oasis HLB and Plexa PCX. Recoveries using Oasis HLB ranges from 51 to 78%, with RSDr from 5 to 15% and RSDR from 5 to 18%. Recoveries using Plexa PCX ranges from 64 to 85% with RSDr from 4 to 11% and RSDR from 7 to 17%..
publishDate 2010
dc.date.none.fl_str_mv 2010-02-18
2010
2010-02-18T00:00:00Z
2018-07-02T09:40:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/10738
TID:202467791
url http://hdl.handle.net/10400.1/10738
identifier_str_mv TID:202467791
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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