Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cord
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/62214 |
Resumo: | Rosette-forming glioneuronal tumor (RGNT) of the IV ventricle is a rare and recently recognized brain tumor entity. It is histologically composed by two distinct features: a glial component, resembling pilocytic astrocytoma, and a component forming neurocytic rosettes and/or perivascular rosettes. Herein, we describe a 33-year-old man with RGNT arising in the spinal cord. Following an immunohistochemistry validation, we further performed an extensive genomic analysis, using array-CGH (aCGH), whole exome and cancer-related hotspot sequencing, in order to better understand its underlying biology. We observed the loss of 1p and gain of 1q, as well as gain of the whole chromosomes 7, 9 and 16. Local amplifications in 9q34.2 and 19p13.3 (encompassing the gene SBNO2) were identified. Moreover, we observed focal gains/losses in several chromosomes. Additionally, on chromosome 7, we identified the presence of the KIAA1549:BRAF gene fusion, which was further validated by RT-PCR and FISH. Across all mutational analyses, we detected and validated the somatic mutations of the genes MLL2, CNNM3, PCDHGC4 and SCN1A. Our comprehensive molecular profiling of this RGNT suggests that MAPK pathway and methylome changes, driven by KIAA1549:BRAF fusion and MLL2 mutation, respectively, could be associated with the development of this rare tumor entity. |
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Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cordAdultBrain NeoplasmsChromosome DeletionExomeHumansMagnetic Resonance ImagingMaleSpinal CordCiências Médicas::Medicina BásicaScience & TechnologyRosette-forming glioneuronal tumor (RGNT) of the IV ventricle is a rare and recently recognized brain tumor entity. It is histologically composed by two distinct features: a glial component, resembling pilocytic astrocytoma, and a component forming neurocytic rosettes and/or perivascular rosettes. Herein, we describe a 33-year-old man with RGNT arising in the spinal cord. Following an immunohistochemistry validation, we further performed an extensive genomic analysis, using array-CGH (aCGH), whole exome and cancer-related hotspot sequencing, in order to better understand its underlying biology. We observed the loss of 1p and gain of 1q, as well as gain of the whole chromosomes 7, 9 and 16. Local amplifications in 9q34.2 and 19p13.3 (encompassing the gene SBNO2) were identified. Moreover, we observed focal gains/losses in several chromosomes. Additionally, on chromosome 7, we identified the presence of the KIAA1549:BRAF gene fusion, which was further validated by RT-PCR and FISH. Across all mutational analyses, we detected and validated the somatic mutations of the genes MLL2, CNNM3, PCDHGC4 and SCN1A. Our comprehensive molecular profiling of this RGNT suggests that MAPK pathway and methylome changes, driven by KIAA1549:BRAF fusion and MLL2 mutation, respectively, could be associated with the development of this rare tumor entity.Conselho Nacional de Desenvolvimento Científico e Tecnológico [475358/2011-2] to RMR (www.cnpq.br); Fundação de Amparo a Pesquisa do Estado de São Paulo [2012/19590-0] to RMR and [2011/08523-7 and 2012/08287-4] to LTB (www.fapesp.br); the Foundation for Science and Technology (FCT) [PTDC/SAU-ONC/115513/2009] to RMR; and the National Cancer Institute [P30CA046934] to MGVPublic Library of Science (PLOS)Universidade do MinhoBidinotto, Lucas TadeuScapulatempo-Neto, CristovamMackay, Alande Almeida, Gisele CaravinaScheithauer, Bernd WalterBerardinelli, Gustavo NorizTorrieri, RaulClara, Carlos AfonsoFeltrin, Leonir TerezinhaPereira, Marta Sofia Carvalho Ribeiro VianaVarella-Garcia, MarileilaJones, ChrisReis, R. M.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/62214eng1932-62031932-620310.1371/journal.pone.013769026371886https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0137690info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:32Zoai:repositorium.sdum.uminho.pt:1822/62214Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:11:22.475962Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cord |
title |
Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cord |
spellingShingle |
Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cord Bidinotto, Lucas Tadeu Adult Brain Neoplasms Chromosome Deletion Exome Humans Magnetic Resonance Imaging Male Spinal Cord Ciências Médicas::Medicina Básica Science & Technology |
title_short |
Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cord |
title_full |
Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cord |
title_fullStr |
Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cord |
title_full_unstemmed |
Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cord |
title_sort |
Molecular profiling of a rare rosette-forming glioneuronal tumor arising in the spinal cord |
author |
Bidinotto, Lucas Tadeu |
author_facet |
Bidinotto, Lucas Tadeu Scapulatempo-Neto, Cristovam Mackay, Alan de Almeida, Gisele Caravina Scheithauer, Bernd Walter Berardinelli, Gustavo Noriz Torrieri, Raul Clara, Carlos Afonso Feltrin, Leonir Terezinha Pereira, Marta Sofia Carvalho Ribeiro Viana Varella-Garcia, Marileila Jones, Chris Reis, R. M. |
author_role |
author |
author2 |
Scapulatempo-Neto, Cristovam Mackay, Alan de Almeida, Gisele Caravina Scheithauer, Bernd Walter Berardinelli, Gustavo Noriz Torrieri, Raul Clara, Carlos Afonso Feltrin, Leonir Terezinha Pereira, Marta Sofia Carvalho Ribeiro Viana Varella-Garcia, Marileila Jones, Chris Reis, R. M. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Bidinotto, Lucas Tadeu Scapulatempo-Neto, Cristovam Mackay, Alan de Almeida, Gisele Caravina Scheithauer, Bernd Walter Berardinelli, Gustavo Noriz Torrieri, Raul Clara, Carlos Afonso Feltrin, Leonir Terezinha Pereira, Marta Sofia Carvalho Ribeiro Viana Varella-Garcia, Marileila Jones, Chris Reis, R. M. |
dc.subject.por.fl_str_mv |
Adult Brain Neoplasms Chromosome Deletion Exome Humans Magnetic Resonance Imaging Male Spinal Cord Ciências Médicas::Medicina Básica Science & Technology |
topic |
Adult Brain Neoplasms Chromosome Deletion Exome Humans Magnetic Resonance Imaging Male Spinal Cord Ciências Médicas::Medicina Básica Science & Technology |
description |
Rosette-forming glioneuronal tumor (RGNT) of the IV ventricle is a rare and recently recognized brain tumor entity. It is histologically composed by two distinct features: a glial component, resembling pilocytic astrocytoma, and a component forming neurocytic rosettes and/or perivascular rosettes. Herein, we describe a 33-year-old man with RGNT arising in the spinal cord. Following an immunohistochemistry validation, we further performed an extensive genomic analysis, using array-CGH (aCGH), whole exome and cancer-related hotspot sequencing, in order to better understand its underlying biology. We observed the loss of 1p and gain of 1q, as well as gain of the whole chromosomes 7, 9 and 16. Local amplifications in 9q34.2 and 19p13.3 (encompassing the gene SBNO2) were identified. Moreover, we observed focal gains/losses in several chromosomes. Additionally, on chromosome 7, we identified the presence of the KIAA1549:BRAF gene fusion, which was further validated by RT-PCR and FISH. Across all mutational analyses, we detected and validated the somatic mutations of the genes MLL2, CNNM3, PCDHGC4 and SCN1A. Our comprehensive molecular profiling of this RGNT suggests that MAPK pathway and methylome changes, driven by KIAA1549:BRAF fusion and MLL2 mutation, respectively, could be associated with the development of this rare tumor entity. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/62214 |
url |
http://hdl.handle.net/1822/62214 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-6203 1932-6203 10.1371/journal.pone.0137690 26371886 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0137690 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science (PLOS) |
publisher.none.fl_str_mv |
Public Library of Science (PLOS) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132546177433600 |