Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons

Detalhes bibliográficos
Autor(a) principal: Correia, Patrícia A
Data de Publicação: 2017
Outros Autores: Lottem, Eran, Banerjee, Dhruba, Machado, Ana S, Carey, Megan R, Mainen, Zachary F
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/23191
Resumo: Serotonin (5-HT) is associated with mood and motivation but the function of endogenous 5-HT remains controversial. Here, we studied the impact of phasic optogenetic activation of 5-HT neurons in mice over time scales from seconds to weeks. We found that activating dorsal raphe nucleus (DRN) 5-HT neurons induced a strong suppression of spontaneous locomotor behavior in the open field with rapid kinetics (onset ≤1 s). Inhibition of locomotion was independent of measures of anxiety or motor impairment and could be overcome by strong motivational drive. Repetitive place-contingent pairing of activation caused neither place preference nor aversion. However, repeated 15 min daily stimulation caused a persistent increase in spontaneous locomotion to emerge over three weeks. These results show that 5-HT transients have strong and opposing short and long-term effects on motor behavior that appear to arise from effects on the underlying factors that motivate actions.
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spelling Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neuronsDorsal Raphe NucleusMiceOptogeneticsSerotoninLocomotionSerotonin (5-HT) is associated with mood and motivation but the function of endogenous 5-HT remains controversial. Here, we studied the impact of phasic optogenetic activation of 5-HT neurons in mice over time scales from seconds to weeks. We found that activating dorsal raphe nucleus (DRN) 5-HT neurons induced a strong suppression of spontaneous locomotor behavior in the open field with rapid kinetics (onset ≤1 s). Inhibition of locomotion was independent of measures of anxiety or motor impairment and could be overcome by strong motivational drive. Repetitive place-contingent pairing of activation caused neither place preference nor aversion. However, repeated 15 min daily stimulation caused a persistent increase in spontaneous locomotion to emerge over three weeks. These results show that 5-HT transients have strong and opposing short and long-term effects on motor behavior that appear to arise from effects on the underlying factors that motivate actions.ERC 250334, 5-HT OptogeneticERC 671251, 5-HT CircuitsRepositório ComumCorreia, Patrícia ALottem, EranBanerjee, DhrubaMachado, Ana SCarey, Megan RMainen, Zachary F2018-06-29T15:07:08Z2017-02-142017-02-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/23191eng10.7554/eLife.20975info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-26T17:15:13Zoai:comum.rcaap.pt:10400.26/23191Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:49:01.204650Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons
title Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons
spellingShingle Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons
Correia, Patrícia A
Dorsal Raphe Nucleus
Mice
Optogenetics
Serotonin
Locomotion
title_short Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons
title_full Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons
title_fullStr Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons
title_full_unstemmed Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons
title_sort Transient inhibition and long-term facilitation of locomotion by phasic optogenetic activation of serotonin neurons
author Correia, Patrícia A
author_facet Correia, Patrícia A
Lottem, Eran
Banerjee, Dhruba
Machado, Ana S
Carey, Megan R
Mainen, Zachary F
author_role author
author2 Lottem, Eran
Banerjee, Dhruba
Machado, Ana S
Carey, Megan R
Mainen, Zachary F
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Comum
dc.contributor.author.fl_str_mv Correia, Patrícia A
Lottem, Eran
Banerjee, Dhruba
Machado, Ana S
Carey, Megan R
Mainen, Zachary F
dc.subject.por.fl_str_mv Dorsal Raphe Nucleus
Mice
Optogenetics
Serotonin
Locomotion
topic Dorsal Raphe Nucleus
Mice
Optogenetics
Serotonin
Locomotion
description Serotonin (5-HT) is associated with mood and motivation but the function of endogenous 5-HT remains controversial. Here, we studied the impact of phasic optogenetic activation of 5-HT neurons in mice over time scales from seconds to weeks. We found that activating dorsal raphe nucleus (DRN) 5-HT neurons induced a strong suppression of spontaneous locomotor behavior in the open field with rapid kinetics (onset ≤1 s). Inhibition of locomotion was independent of measures of anxiety or motor impairment and could be overcome by strong motivational drive. Repetitive place-contingent pairing of activation caused neither place preference nor aversion. However, repeated 15 min daily stimulation caused a persistent increase in spontaneous locomotion to emerge over three weeks. These results show that 5-HT transients have strong and opposing short and long-term effects on motor behavior that appear to arise from effects on the underlying factors that motivate actions.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-14
2017-02-14T00:00:00Z
2018-06-29T15:07:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/23191
url http://hdl.handle.net/10400.26/23191
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.7554/eLife.20975
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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