The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models

Detalhes bibliográficos
Autor(a) principal: Rosignol, Ines
Data de Publicação: 2020
Outros Autores: Villarejo-Zori, Beatriz, Teresak, Petra, Sierra-Filardi, Elena, Pereiro, Xandra, Rodríguez-Muela, Natalia, Vecino, Elena, Vieira, Helena L.A., Bell, Katharina, Boya, Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/95324
Resumo: This research was funded by Ministerio de Ciencia, Innovación y Universidades (MCIU), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) PGC2018-098557-B-I00 and European Union’s Horizon 2020 research and innovation programme under grant agreement No 765912. BVZ is a recipient of PhD contract from the Fundación Tatiana Pérez de Guzmán el Bueno (Spain), PT from H2020-MSCA-ITN-2017, NRM a Juan de la Cierva Grant from Ministerio Ciencia e Innovación (Spain) and KB from DFG (Deutsche Forschungsgemeinschaft, Germany, 6619/1-1).
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spelling The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma modelsAutophagyCell deathGlaucomaMito-QC reporterMitophagyPrimary neuronal cell cultureRetinal ganglion cellsCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistryThis research was funded by Ministerio de Ciencia, Innovación y Universidades (MCIU), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) PGC2018-098557-B-I00 and European Union’s Horizon 2020 research and innovation programme under grant agreement No 765912. BVZ is a recipient of PhD contract from the Fundación Tatiana Pérez de Guzmán el Bueno (Spain), PT from H2020-MSCA-ITN-2017, NRM a Juan de la Cierva Grant from Ministerio Ciencia e Innovación (Spain) and KB from DFG (Deutsche Forschungsgemeinschaft, Germany, 6619/1-1).Mitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to date limited tools are available for analyzing mitophagy in vivo. We have taken advantage of the mito-QC reporter, a recently generated mouse model that allows an accurate mitophagy assessment to fill this gap. We used primary RGCs and retinal explants derived from mito-QC mice to quantify mitophagy activation in vitro and ex vivo. We also analyzed mitophagy in retinal ganglion cells (RGCs), in vivo, using different mitophagy inducers, as well as after optic nerve crush (ONC) in mice, a commonly used surgical procedure to model glaucoma. Using mito-QC reporter we quantified mitophagy induced by several known inducers in primary RGCs in vitro, ex vivo and in vivo. We also found that RGCs were rescued from some glaucoma relevant stress factors by incubation with the iron chelator deferiprone (DFP). Thus, the mito-QC reporter-based model is a valuable tool for accurately analyzing mitophagy in the context of glaucoma.UCIBIO - Applied Molecular Biosciences UnitCentro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Faculdade de Ciências e Tecnologia (FCT)RUNRosignol, InesVillarejo-Zori, BeatrizTeresak, PetraSierra-Filardi, ElenaPereiro, XandraRodríguez-Muela, NataliaVecino, ElenaVieira, Helena L.A.Bell, KatharinaBoya, Patricia2020-03-30T22:17:55Z2020-03-012020-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/95324eng1661-6596PURE: 17542355https://doi.org/10.3390/ijms21051882info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:43:19Zoai:run.unl.pt:10362/95324Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:38:17.686431Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models
title The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models
spellingShingle The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models
Rosignol, Ines
Autophagy
Cell death
Glaucoma
Mito-QC reporter
Mitophagy
Primary neuronal cell culture
Retinal ganglion cells
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
title_short The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models
title_full The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models
title_fullStr The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models
title_full_unstemmed The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models
title_sort The mito-QC reporter for quantitative mitophagy assessment in primary retinal ganglion cells and experimental glaucoma models
author Rosignol, Ines
author_facet Rosignol, Ines
Villarejo-Zori, Beatriz
Teresak, Petra
Sierra-Filardi, Elena
Pereiro, Xandra
Rodríguez-Muela, Natalia
Vecino, Elena
Vieira, Helena L.A.
Bell, Katharina
Boya, Patricia
author_role author
author2 Villarejo-Zori, Beatriz
Teresak, Petra
Sierra-Filardi, Elena
Pereiro, Xandra
Rodríguez-Muela, Natalia
Vecino, Elena
Vieira, Helena L.A.
Bell, Katharina
Boya, Patricia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Faculdade de Ciências e Tecnologia (FCT)
RUN
dc.contributor.author.fl_str_mv Rosignol, Ines
Villarejo-Zori, Beatriz
Teresak, Petra
Sierra-Filardi, Elena
Pereiro, Xandra
Rodríguez-Muela, Natalia
Vecino, Elena
Vieira, Helena L.A.
Bell, Katharina
Boya, Patricia
dc.subject.por.fl_str_mv Autophagy
Cell death
Glaucoma
Mito-QC reporter
Mitophagy
Primary neuronal cell culture
Retinal ganglion cells
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
topic Autophagy
Cell death
Glaucoma
Mito-QC reporter
Mitophagy
Primary neuronal cell culture
Retinal ganglion cells
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
description This research was funded by Ministerio de Ciencia, Innovación y Universidades (MCIU), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER) PGC2018-098557-B-I00 and European Union’s Horizon 2020 research and innovation programme under grant agreement No 765912. BVZ is a recipient of PhD contract from the Fundación Tatiana Pérez de Guzmán el Bueno (Spain), PT from H2020-MSCA-ITN-2017, NRM a Juan de la Cierva Grant from Ministerio Ciencia e Innovación (Spain) and KB from DFG (Deutsche Forschungsgemeinschaft, Germany, 6619/1-1).
publishDate 2020
dc.date.none.fl_str_mv 2020-03-30T22:17:55Z
2020-03-01
2020-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/95324
url http://hdl.handle.net/10362/95324
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1661-6596
PURE: 17542355
https://doi.org/10.3390/ijms21051882
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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