Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approach
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/22653 |
Resumo: | The Ca2+-induced cold gelation technique was found suitable to prepare highly porous biodegradable scaffolds based on bovine serum albumin (BSA) and alpha-casein from bovine milk for tissue engineering. A 23 full factorial design was used to study the influence and impact of each factor on the several responses of the scaffolds. In vitro degradation (ID), swelling ratio (SR), porosity (PO), and pore size (PS) as well cytotoxicity (CT) were evaluated and shown to be dependent on the pH of sample preparation and on the amount of BSA and casein present, making these scaffolds tunable structures. Under optimized working conditions (4.19% of BSA, 0.69% of Casein, pH 7.07), the ID attained was 37.97%, the SR observed was 11.87, the PO was 82.11%, the PS measured was 180.63 μm at surface, and 175.91 μm at fracture, whereas maximum cell viability was 84% in comparison to controls. Moreover, the scaffold supported cell adhesion and proliferation. These results, consistent with the prediction by the experimental design approach, support the use of this methodology to develop tunable scaffolds for tissue engineering using the Ca2+-induced cold gelation. |
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Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approachCold gelationScaffoldsExperimental designPorosityTissue engineeringScience & TechnologyThe Ca2+-induced cold gelation technique was found suitable to prepare highly porous biodegradable scaffolds based on bovine serum albumin (BSA) and alpha-casein from bovine milk for tissue engineering. A 23 full factorial design was used to study the influence and impact of each factor on the several responses of the scaffolds. In vitro degradation (ID), swelling ratio (SR), porosity (PO), and pore size (PS) as well cytotoxicity (CT) were evaluated and shown to be dependent on the pH of sample preparation and on the amount of BSA and casein present, making these scaffolds tunable structures. Under optimized working conditions (4.19% of BSA, 0.69% of Casein, pH 7.07), the ID attained was 37.97%, the SR observed was 11.87, the PO was 82.11%, the PS measured was 180.63 μm at surface, and 175.91 μm at fracture, whereas maximum cell viability was 84% in comparison to controls. Moreover, the scaffold supported cell adhesion and proliferation. These results, consistent with the prediction by the experimental design approach, support the use of this methodology to develop tunable scaffolds for tissue engineering using the Ca2+-induced cold gelation.Contract grant sponsor: FEDER through POFC-COMPETE and by national funds from FCT; contract grant number: PEst-C/BIA/UI4050/2011WileyUniversidade do MinhoRibeiro, Artur J.Gomes, Andreia CPaulo, Artur Cavaco20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/22653eng1552-498110.1002/jbm.b.3279722987762http://dx.doi.org/10.1002/jbm.b.32797info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-07T01:22:17Zoai:repositorium.sdum.uminho.pt:1822/22653Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:47:22.495679Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approach |
title |
Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approach |
spellingShingle |
Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approach Ribeiro, Artur J. Cold gelation Scaffolds Experimental design Porosity Tissue engineering Science & Technology |
title_short |
Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approach |
title_full |
Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approach |
title_fullStr |
Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approach |
title_full_unstemmed |
Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approach |
title_sort |
Developing scaffolds for tissue engineering using the Ca2+-induced cold gelation by an experimental design approach |
author |
Ribeiro, Artur J. |
author_facet |
Ribeiro, Artur J. Gomes, Andreia C Paulo, Artur Cavaco |
author_role |
author |
author2 |
Gomes, Andreia C Paulo, Artur Cavaco |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ribeiro, Artur J. Gomes, Andreia C Paulo, Artur Cavaco |
dc.subject.por.fl_str_mv |
Cold gelation Scaffolds Experimental design Porosity Tissue engineering Science & Technology |
topic |
Cold gelation Scaffolds Experimental design Porosity Tissue engineering Science & Technology |
description |
The Ca2+-induced cold gelation technique was found suitable to prepare highly porous biodegradable scaffolds based on bovine serum albumin (BSA) and alpha-casein from bovine milk for tissue engineering. A 23 full factorial design was used to study the influence and impact of each factor on the several responses of the scaffolds. In vitro degradation (ID), swelling ratio (SR), porosity (PO), and pore size (PS) as well cytotoxicity (CT) were evaluated and shown to be dependent on the pH of sample preparation and on the amount of BSA and casein present, making these scaffolds tunable structures. Under optimized working conditions (4.19% of BSA, 0.69% of Casein, pH 7.07), the ID attained was 37.97%, the SR observed was 11.87, the PO was 82.11%, the PS measured was 180.63 μm at surface, and 175.91 μm at fracture, whereas maximum cell viability was 84% in comparison to controls. Moreover, the scaffold supported cell adhesion and proliferation. These results, consistent with the prediction by the experimental design approach, support the use of this methodology to develop tunable scaffolds for tissue engineering using the Ca2+-induced cold gelation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/22653 |
url |
https://hdl.handle.net/1822/22653 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1552-4981 10.1002/jbm.b.32797 22987762 http://dx.doi.org/10.1002/jbm.b.32797 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133046962651136 |