Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous material

Detalhes bibliográficos
Autor(a) principal: Fassini, Dario
Data de Publicação: 2017
Outros Autores: Duarte, Ana Rita C., Reis, R. L., Silva, Tiago José Quinteiros Lopes Henriques
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/48150
Resumo: Collagen is a natural and abundant polymer that serves multiple functions in both invertebrates and vertebrates. As collagen is the natural scaffolding for cells, collagen-based hydrogels are regarded as ideal materials for tissue engineering applications since they can mimic the natural cellular microenvironment. Chondrosia reniformis is a marine demosponge particularly rich in collagen, characterized by the presence of labile interfibrillar crosslinks similarly to those described in the mutable collagenous tissues (MCTs) of echinoderms. As a result single fibrils can be isolated using calcium-chelating and disulphide-reducing chemicals. In the present work we firstly describe a new extraction method that directly produces a highly hydrated hydrogel with interesting self-healing properties. The materials obtained were then biochemically and rheologically characterized. Our investigation has shown that the developed extraction procedure is able to extract collagen as well as other proteins and Glycosaminoglycans (GAG)-like molecules that give the collagenous hydrogel interesting and new rheological properties when compared to other described collagenous materials. The present work motivates further in-depth investigations towards the development of a new class of injectable collagenous hydrogels with tailored specifications.
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spelling Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous materialChondrosia reniformisCollagen rheologyHydrogelMarine biomaterialsMarine collagenMarine sponge GAGScience & TechnologyCollagen is a natural and abundant polymer that serves multiple functions in both invertebrates and vertebrates. As collagen is the natural scaffolding for cells, collagen-based hydrogels are regarded as ideal materials for tissue engineering applications since they can mimic the natural cellular microenvironment. Chondrosia reniformis is a marine demosponge particularly rich in collagen, characterized by the presence of labile interfibrillar crosslinks similarly to those described in the mutable collagenous tissues (MCTs) of echinoderms. As a result single fibrils can be isolated using calcium-chelating and disulphide-reducing chemicals. In the present work we firstly describe a new extraction method that directly produces a highly hydrated hydrogel with interesting self-healing properties. The materials obtained were then biochemically and rheologically characterized. Our investigation has shown that the developed extraction procedure is able to extract collagen as well as other proteins and Glycosaminoglycans (GAG)-like molecules that give the collagenous hydrogel interesting and new rheological properties when compared to other described collagenous materials. The present work motivates further in-depth investigations towards the development of a new class of injectable collagenous hydrogels with tailored specifications.The authors gratefully acknowledge the financial support from the European Union Seventh Framework Programme (FP7/2007–2013) under grant agreement ERC-2012-ADG 20120216-321266 (ERC Advanced Grant project ComplexiTE), as well as from the European Regional Development Fund (ERDF) under the projects “Accelerating tissue engineering and personalized medicine discoveries by the integration of key enabling nanotechonologies, marine-derived biomaterials and stem cells” (NORTE-01-0145-FEDER-000021), supported by Norte Portugal Regional Operational Program (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, and 0687_NOVOMAR_1_P, co-financed by transborder cooperation programme POCTEP. The authors are also thankful to the Area Marina Protetta Portofino (Italy) for permission to collect sponge specimens and to Daniela Candia (University of Milan, Italy) and Marco Giovine (University of Genoa, Italy) for the logistical support on the sponge sampling and immediate processing. We are grateful to the two anonymous referees for improving the quality of the present article.info:eu-repo/semantics/publishedVersionMultidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoFassini, DarioDuarte, Ana Rita C.Reis, R. L.Silva, Tiago José Quinteiros Lopes Henriques2017-122017-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/48150engFassini D., Duarte A. R. C., Reis R. L., Silva T. H. Bioinspiring Chondrosia reniformis (Nardo, 1847) Collagen-Based Hydrogel: A New Extraction Method to Obtain a Sticky and Self-Healing Collagenous Material, Marine Drugs, Vol. 15, Issue 12, pp. 380-397, doi:10.3390/md15120380, 20171660-339710.3390/md1512038029207538http://www.mdpi.com/1660-3397/15/12/380info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:36Zoai:repositorium.sdum.uminho.pt:1822/48150Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:54.876038Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous material
title Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous material
spellingShingle Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous material
Fassini, Dario
Chondrosia reniformis
Collagen rheology
Hydrogel
Marine biomaterials
Marine collagen
Marine sponge GAG
Science & Technology
title_short Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous material
title_full Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous material
title_fullStr Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous material
title_full_unstemmed Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous material
title_sort Bioinspiring Chondrosia reniformis (Nardo, 1847) collagen-based hydrogel: a new extraction method to obtain a sticky and self-healing collagenous material
author Fassini, Dario
author_facet Fassini, Dario
Duarte, Ana Rita C.
Reis, R. L.
Silva, Tiago José Quinteiros Lopes Henriques
author_role author
author2 Duarte, Ana Rita C.
Reis, R. L.
Silva, Tiago José Quinteiros Lopes Henriques
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Fassini, Dario
Duarte, Ana Rita C.
Reis, R. L.
Silva, Tiago José Quinteiros Lopes Henriques
dc.subject.por.fl_str_mv Chondrosia reniformis
Collagen rheology
Hydrogel
Marine biomaterials
Marine collagen
Marine sponge GAG
Science & Technology
topic Chondrosia reniformis
Collagen rheology
Hydrogel
Marine biomaterials
Marine collagen
Marine sponge GAG
Science & Technology
description Collagen is a natural and abundant polymer that serves multiple functions in both invertebrates and vertebrates. As collagen is the natural scaffolding for cells, collagen-based hydrogels are regarded as ideal materials for tissue engineering applications since they can mimic the natural cellular microenvironment. Chondrosia reniformis is a marine demosponge particularly rich in collagen, characterized by the presence of labile interfibrillar crosslinks similarly to those described in the mutable collagenous tissues (MCTs) of echinoderms. As a result single fibrils can be isolated using calcium-chelating and disulphide-reducing chemicals. In the present work we firstly describe a new extraction method that directly produces a highly hydrated hydrogel with interesting self-healing properties. The materials obtained were then biochemically and rheologically characterized. Our investigation has shown that the developed extraction procedure is able to extract collagen as well as other proteins and Glycosaminoglycans (GAG)-like molecules that give the collagenous hydrogel interesting and new rheological properties when compared to other described collagenous materials. The present work motivates further in-depth investigations towards the development of a new class of injectable collagenous hydrogels with tailored specifications.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
2017-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/48150
url http://hdl.handle.net/1822/48150
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Fassini D., Duarte A. R. C., Reis R. L., Silva T. H. Bioinspiring Chondrosia reniformis (Nardo, 1847) Collagen-Based Hydrogel: A New Extraction Method to Obtain a Sticky and Self-Healing Collagenous Material, Marine Drugs, Vol. 15, Issue 12, pp. 380-397, doi:10.3390/md15120380, 2017
1660-3397
10.3390/md15120380
29207538
http://www.mdpi.com/1660-3397/15/12/380
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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