Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/23164 |
Resumo: | © 2015 FEBS |
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7160 |
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Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storageDNAManganeseOligomeric stateOxidative stressReactive oxygen species© 2015 FEBSThe DNA binding proteins from starved cells from Deinococcus radiodurans, Dps1-DR2263 and Dps2-DRB0092, have a common overall structure of hollow spherical dodecamers. Their involvement in the homeostasis of intracellular metal and DNA protection was addressed. Our results show that DrDps proteins are able to oxidize ferrous to ferric iron by oxygen or hydrogen peroxide. The iron stored inside the hollow sphere cavity is fully released. Furthermore, these proteins are able to store and release manganese, suggesting they can play a role in manganese homeostasis as well. The interaction of DrDps with DNA was also addressed. Even though DrDps1 binds both linear and coiled DNA, DrDps2 preferentially binds to coiled DNA, forming different protein–DNA complexes, as clearly shown by atomic force microscopy. DrDps1 (dimer and dodecamer) and DrDps2 can protect DNA against reactive oxygen species, although the protection occurs at different Fe to protein ratios. The difference between DrDps could be the result of the DrDps1 higher iron oxidation rate in the presence of hydrogen peroxide and its higher affinity to bind DNA than in DrDps2. Using cellular extracts obtained from D. radiodurans cultures, we showed that DrDps1 oligomers observed in in vitro conditions are also present in vivo. This indicates that DrDps1 has a structural dynamic plasticity that allows its oligomeric state to change between dimer, trimer and dodecamer. This in turn suggests the existence of a regulation mechanism that modulates the oligomer equilibrium and is dependent on growth stages and environmental conditions.This work was financed by Fundação para a Ciência e Tecnologia through the following Grants: PTDC/BIA-PRO/100365/2008 (C.V.R.), PTDC/QUI-BIQ/100007/2008 (I.A.A.) and PEst-OE/EQB/LA0004/2011. S.P.S. is recipient of PhD Grant PEst-OE/EQB/LA0004/2011. I.A.A. and C.V.R. are recipients of the grants SFRH/BPD/78314/2011 and SFRH/BPD/94050/2013, respectively.WileyRepositório da Universidade de LisboaSantos, Sandra P.Mitchell, Edward P.Franquelim, Henri G.Castanho, Miguel A. R. B.Abreu, Isabel A.Romão, Célia V.2016-03-30T11:22:27Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/23164engFEBS Journal 282 (2015) 4307–43271742-464Xdoi:10.1111/febs.13420metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:27:48Zoai:repositorio.ul.pt:10451/23164Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:27:48Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage |
title |
Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage |
spellingShingle |
Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage Santos, Sandra P. DNA Manganese Oligomeric state Oxidative stress Reactive oxygen species |
title_short |
Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage |
title_full |
Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage |
title_fullStr |
Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage |
title_full_unstemmed |
Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage |
title_sort |
Dps from Deinococcus radiodurans : oligomeric forms of Dps1 with distinct cellular functions and Dps2 involved in metal storage |
author |
Santos, Sandra P. |
author_facet |
Santos, Sandra P. Mitchell, Edward P. Franquelim, Henri G. Castanho, Miguel A. R. B. Abreu, Isabel A. Romão, Célia V. |
author_role |
author |
author2 |
Mitchell, Edward P. Franquelim, Henri G. Castanho, Miguel A. R. B. Abreu, Isabel A. Romão, Célia V. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Santos, Sandra P. Mitchell, Edward P. Franquelim, Henri G. Castanho, Miguel A. R. B. Abreu, Isabel A. Romão, Célia V. |
dc.subject.por.fl_str_mv |
DNA Manganese Oligomeric state Oxidative stress Reactive oxygen species |
topic |
DNA Manganese Oligomeric state Oxidative stress Reactive oxygen species |
description |
© 2015 FEBS |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2016-03-30T11:22:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/23164 |
url |
http://hdl.handle.net/10451/23164 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
FEBS Journal 282 (2015) 4307–4327 1742-464X doi:10.1111/febs.13420 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817548896432291840 |