Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERS

Detalhes bibliográficos
Autor(a) principal: Ramos, Sara Miguel do Vale Teixeira
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/11156
Resumo: Nowadays there is a global trend towards the efficient utilization of natural resources, among others those that are found in food consumed by humans. This situation happens not only as a method to prevent pollution, but also as a practice to benefit from the compounds in several nourishments that can bring benefits to human health. Taking that into consideration, this work intended to evaluate the antioxidant capacity and phenolic profile of apple species available in the Portuguese market, as apple is one of the most consumed fruit around the world. For this purpose, the extraction conditions of apple antioxidants by QuEChERS were developed and optimized. The results obtained were compared to a conventional extraction method so that it was possible to determine which parameters had the greatest influence in the extraction yield. The extraction efficiency was evaluated by the spectrophotometric methods, total phenolic content (TPC), total flavonoid content (TFC) and ferric reducing antioxidant power (FRAP), and the phenolic profile was assessed by high performance liquid chromatography with diode array detection (HPLC-DAD). In comparison with the conventional extraction, the higher results were obtained with the QuEChERS extraction with salt S1, composed by magnesium sulphate and sodium acetate, which was able to promote the extraction and therefore the amount of extracted phenolic compounds. For the solvents tested, water was the extractor solvent that enabled to obtain the highest yield, either by conventional extraction (TPC – 154.4 ± 5.9 µg GAE/g; TFC – 98.7 ± 7.3 µg EE/g; FRAP – 71.7 ± 1.4 µg AAE/g) or by QuEChERS extraction (TPC – 505.5 ± 63.7 µg GAE/g; TFC – 881.6 ± 69.9 µg EE/g; FRAP – 25.7 ± 2.5 µg AAE/g). Regarding the phenolic profile assessment, in the QuEChERS extract was possible to identify several acids, namely chlorogenic, caffeic, vanillic, p-coumaric acid and sinapic acids and only the flavonoid naringin. In the conventional extracts, the phenolic compounds identified were the same as for the QuEChERS extracts, although in the extract from acetonitrile solvent it was possible to quantify naringin and β-resorcyclic acid with the amounts of 0.64 ± 0.03 and 1.94 ± 0.09 mg/L, respectively.
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spelling Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERSAntioxidantsQuEChERSApplePhenolicsFlavonoidsSpectrophotometric methodsHPLC-DADAntioxidantesMaçãFenólicosFlavonoidesMétodos espectrofotométricosOptimização Energética na Indústria QuímicaNowadays there is a global trend towards the efficient utilization of natural resources, among others those that are found in food consumed by humans. This situation happens not only as a method to prevent pollution, but also as a practice to benefit from the compounds in several nourishments that can bring benefits to human health. Taking that into consideration, this work intended to evaluate the antioxidant capacity and phenolic profile of apple species available in the Portuguese market, as apple is one of the most consumed fruit around the world. For this purpose, the extraction conditions of apple antioxidants by QuEChERS were developed and optimized. The results obtained were compared to a conventional extraction method so that it was possible to determine which parameters had the greatest influence in the extraction yield. The extraction efficiency was evaluated by the spectrophotometric methods, total phenolic content (TPC), total flavonoid content (TFC) and ferric reducing antioxidant power (FRAP), and the phenolic profile was assessed by high performance liquid chromatography with diode array detection (HPLC-DAD). In comparison with the conventional extraction, the higher results were obtained with the QuEChERS extraction with salt S1, composed by magnesium sulphate and sodium acetate, which was able to promote the extraction and therefore the amount of extracted phenolic compounds. For the solvents tested, water was the extractor solvent that enabled to obtain the highest yield, either by conventional extraction (TPC – 154.4 ± 5.9 µg GAE/g; TFC – 98.7 ± 7.3 µg EE/g; FRAP – 71.7 ± 1.4 µg AAE/g) or by QuEChERS extraction (TPC – 505.5 ± 63.7 µg GAE/g; TFC – 881.6 ± 69.9 µg EE/g; FRAP – 25.7 ± 2.5 µg AAE/g). Regarding the phenolic profile assessment, in the QuEChERS extract was possible to identify several acids, namely chlorogenic, caffeic, vanillic, p-coumaric acid and sinapic acids and only the flavonoid naringin. In the conventional extracts, the phenolic compounds identified were the same as for the QuEChERS extracts, although in the extract from acetonitrile solvent it was possible to quantify naringin and β-resorcyclic acid with the amounts of 0.64 ± 0.03 and 1.94 ± 0.09 mg/L, respectively.Atualmente, existe uma tendência global para utilizar de forma eficiente os recursos naturais, entre outros os que são encontrados nos alimentos consumidos pela população. Tal situação sucede não só como um método de prevenção da poluição, mas também como um meio para beneficiar de compostos presentes nos vários alimentos que poderão trazer benefícios para a saúde humana. Tendo isto em consideração, este trabalho teve como objetivo avaliar a capacidade antioxidante e o perfil fenólico de espécies de maçãs disponíveis no mercado português, sendo a maçã um dos alimentos mais consumidos por todo o mundo. Para o efeito foram desenvolvidas e otimizadas as condições de extração de antioxidantes da maçã por QuEChERS. Comparou-se os resultados obtidos com um método de extração convencional, de tal forma que fosse possível determinar quais os parâmetros que mais influenciam o rendimento da extração. A eficiência da extração foi avaliada através de métodos espetrofotométricos, teor de fenólicos totais (TPC), teor de flavonoides totais (TFC) e método de redução do ferro (FRAP), e o perfil fenólico foi avaliado por cromatografia de alta eficiência com deteção por um sistema de díodos (HPLC-DAD). Em comparação com a extração convencional, os melhores resultados foram obtidos na extração por QuEChERS com o sal S1, composto por sulfato de magnésio e acetato de sódio, que por sua vez foi capaz de favorecer a extração e, consequentemente, a quantidade de compostos extraídos. Relativamente aos solventes estudados, a água foi o solvente de extração que permitiu obter o rendimento mais elevado, tanto pelo método convencional (TPC – 154,4 ± 5,9 µg GAE/g; TFC – 98,7 ± 7,3 µg EE/g; FRAP – 71,7 ± 1,4 µg AAE/g) como por QuEChERS (TPC – 505,5 ± 63,7 µg GAE/g; TFC – 881,6 ± 69,9 µg EE/g; FRAP – 25,7 ± 2,5 µg AAE/g). Em relação à avaliação do perfil fenólico, na extração por QuEChERS foi possível identificar vários ácidos, nomeadamente ácido clorogénico, ácido cafeico, ácido vanílico, ácido pcumárico e ácido sinápico, e apenas o flavonoide naringina. Na extração convencional os compostos fenólicos identificados foram os mesmos que na extração por QuEChERS, embora nos extratos com o solvente acetonitrilo tivesse sido possível quantificar a naringina e o ácido β-resorcílico com resultados de 0,64 ± 0,03 e 1,94 ± 0,09 mg/L, respetivamente.Ferreira, Maria João Dantas RamalhosaRepositório Científico do Instituto Politécnico do PortoRamos, Sara Miguel do Vale Teixeira2018-03-19T16:24:52Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.22/11156TID:201750830enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:53:14Zoai:recipp.ipp.pt:10400.22/11156Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:31:32.914825Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERS
title Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERS
spellingShingle Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERS
Ramos, Sara Miguel do Vale Teixeira
Antioxidants
QuEChERS
Apple
Phenolics
Flavonoids
Spectrophotometric methods
HPLC-DAD
Antioxidantes
Maçã
Fenólicos
Flavonoides
Métodos espectrofotométricos
Optimização Energética na Indústria Química
title_short Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERS
title_full Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERS
title_fullStr Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERS
title_full_unstemmed Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERS
title_sort Desenvolvimento e otimização de extração de antioxidantes de matrizes alimentares por QuEChERS
author Ramos, Sara Miguel do Vale Teixeira
author_facet Ramos, Sara Miguel do Vale Teixeira
author_role author
dc.contributor.none.fl_str_mv Ferreira, Maria João Dantas Ramalhosa
Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Ramos, Sara Miguel do Vale Teixeira
dc.subject.por.fl_str_mv Antioxidants
QuEChERS
Apple
Phenolics
Flavonoids
Spectrophotometric methods
HPLC-DAD
Antioxidantes
Maçã
Fenólicos
Flavonoides
Métodos espectrofotométricos
Optimização Energética na Indústria Química
topic Antioxidants
QuEChERS
Apple
Phenolics
Flavonoids
Spectrophotometric methods
HPLC-DAD
Antioxidantes
Maçã
Fenólicos
Flavonoides
Métodos espectrofotométricos
Optimização Energética na Indústria Química
description Nowadays there is a global trend towards the efficient utilization of natural resources, among others those that are found in food consumed by humans. This situation happens not only as a method to prevent pollution, but also as a practice to benefit from the compounds in several nourishments that can bring benefits to human health. Taking that into consideration, this work intended to evaluate the antioxidant capacity and phenolic profile of apple species available in the Portuguese market, as apple is one of the most consumed fruit around the world. For this purpose, the extraction conditions of apple antioxidants by QuEChERS were developed and optimized. The results obtained were compared to a conventional extraction method so that it was possible to determine which parameters had the greatest influence in the extraction yield. The extraction efficiency was evaluated by the spectrophotometric methods, total phenolic content (TPC), total flavonoid content (TFC) and ferric reducing antioxidant power (FRAP), and the phenolic profile was assessed by high performance liquid chromatography with diode array detection (HPLC-DAD). In comparison with the conventional extraction, the higher results were obtained with the QuEChERS extraction with salt S1, composed by magnesium sulphate and sodium acetate, which was able to promote the extraction and therefore the amount of extracted phenolic compounds. For the solvents tested, water was the extractor solvent that enabled to obtain the highest yield, either by conventional extraction (TPC – 154.4 ± 5.9 µg GAE/g; TFC – 98.7 ± 7.3 µg EE/g; FRAP – 71.7 ± 1.4 µg AAE/g) or by QuEChERS extraction (TPC – 505.5 ± 63.7 µg GAE/g; TFC – 881.6 ± 69.9 µg EE/g; FRAP – 25.7 ± 2.5 µg AAE/g). Regarding the phenolic profile assessment, in the QuEChERS extract was possible to identify several acids, namely chlorogenic, caffeic, vanillic, p-coumaric acid and sinapic acids and only the flavonoid naringin. In the conventional extracts, the phenolic compounds identified were the same as for the QuEChERS extracts, although in the extract from acetonitrile solvent it was possible to quantify naringin and β-resorcyclic acid with the amounts of 0.64 ± 0.03 and 1.94 ± 0.09 mg/L, respectively.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2018-03-19T16:24:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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TID:201750830
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dc.language.iso.fl_str_mv eng
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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