Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/50332 |
Resumo: | Copyright: © 2019 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Cell sources of inflammatory mediators present in bone marrow areas inside the meniscusCopyright: © 2019 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Purpose: To demonstrate the production of inflammatory mediators by cells located in bone marrow spaces inside rodent menisci. Methods: Mice subjected to transection of the medial collateral and anterior cruciate ligaments and meniscotomy (osteoarthritis model) or to a sham procedure, as well as non-operated (naive) mice and rats, had knee joints excised. Tissues were stained with hematoxylin-eosin and tartrate-resistant acid phosphatase (TRAP). CD68+ cells, inducible nitric oxide synthase (iNOS), interleukin (IL)-1β, and tumor necrosis factor (TNF) expression were detected using immunohistochemistry. Results: Lamellar ossified areas, bone-entrapped osteocytes and bone marrow spaces were found inside menisci of one week up to 6 months-old naïve mice, regardless of gender. Menisci from naive rats also showed the same pattern with bone marrow areas. CD68+ cells were identified in bone marrow areas inside the meniscus of mice. TRAP+ osteoclasts, and hematogenous precursors expressing IL-1β, TNF, and iNOS were identified inside bone marrow areas in meniscal samples from both naïve and sham operated mice. Quantitative immunoexpression of IL-1 β, TNF and iNOS was more intense, P = 0.0194, 0.0293, 0.0124, respectively, in mouse knees from mice sacrificed 49 days after being subjected to an osteoarthritis (OA) model as compared to sham operated animals. Conclusion: We provide novel data showing that rodent menisci display bone marrow areas with cells able to produce inflammatory mediators. Immunoexpression of inflammatory mediators in those bone marrow areas is significantly more pronounced in mice subjected to experimental OA.The study was funded by: UID/BIM/50005/2019, project funded by Fundação para a Ciência e a Tecnologia (FCT)/ Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through Fundos do Orçamento de Estado.PLOSRepositório da Universidade de LisboaRocha, Francisco Airton CastroGirão, Virgínia Claudia CarneiroNunes, Rodolfo de MeloPinto, Ana Carolina Matias DinellyVidal, BrunoFonseca, João Eurico2021-12-10T14:13:38Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/50332engPLoS One. 2019 Dec 20;14(12):e022698610.1371/journal.pone.02269861932-6203info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T18:10:26Zoai:repositorio.ul.pt:10451/50332Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T18:10:26Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus |
title |
Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus |
spellingShingle |
Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus Rocha, Francisco Airton Castro |
title_short |
Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus |
title_full |
Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus |
title_fullStr |
Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus |
title_full_unstemmed |
Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus |
title_sort |
Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus |
author |
Rocha, Francisco Airton Castro |
author_facet |
Rocha, Francisco Airton Castro Girão, Virgínia Claudia Carneiro Nunes, Rodolfo de Melo Pinto, Ana Carolina Matias Dinelly Vidal, Bruno Fonseca, João Eurico |
author_role |
author |
author2 |
Girão, Virgínia Claudia Carneiro Nunes, Rodolfo de Melo Pinto, Ana Carolina Matias Dinelly Vidal, Bruno Fonseca, João Eurico |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Rocha, Francisco Airton Castro Girão, Virgínia Claudia Carneiro Nunes, Rodolfo de Melo Pinto, Ana Carolina Matias Dinelly Vidal, Bruno Fonseca, João Eurico |
description |
Copyright: © 2019 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z 2021-12-10T14:13:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/50332 |
url |
http://hdl.handle.net/10451/50332 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS One. 2019 Dec 20;14(12):e0226986 10.1371/journal.pone.0226986 1932-6203 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
PLOS |
publisher.none.fl_str_mv |
PLOS |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
_version_ |
1817549161348726784 |