Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus

Detalhes bibliográficos
Autor(a) principal: Rocha, Francisco Airton Castro
Data de Publicação: 2019
Outros Autores: Girão, Virgínia Claudia Carneiro, Nunes, Rodolfo de Melo, Pinto, Ana Carolina Matias Dinelly, Vidal, Bruno, Fonseca, João Eurico
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/50332
Resumo: Copyright: © 2019 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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spelling Cell sources of inflammatory mediators present in bone marrow areas inside the meniscusCopyright: © 2019 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Purpose: To demonstrate the production of inflammatory mediators by cells located in bone marrow spaces inside rodent menisci. Methods: Mice subjected to transection of the medial collateral and anterior cruciate ligaments and meniscotomy (osteoarthritis model) or to a sham procedure, as well as non-operated (naive) mice and rats, had knee joints excised. Tissues were stained with hematoxylin-eosin and tartrate-resistant acid phosphatase (TRAP). CD68+ cells, inducible nitric oxide synthase (iNOS), interleukin (IL)-1β, and tumor necrosis factor (TNF) expression were detected using immunohistochemistry. Results: Lamellar ossified areas, bone-entrapped osteocytes and bone marrow spaces were found inside menisci of one week up to 6 months-old naïve mice, regardless of gender. Menisci from naive rats also showed the same pattern with bone marrow areas. CD68+ cells were identified in bone marrow areas inside the meniscus of mice. TRAP+ osteoclasts, and hematogenous precursors expressing IL-1β, TNF, and iNOS were identified inside bone marrow areas in meniscal samples from both naïve and sham operated mice. Quantitative immunoexpression of IL-1 β, TNF and iNOS was more intense, P = 0.0194, 0.0293, 0.0124, respectively, in mouse knees from mice sacrificed 49 days after being subjected to an osteoarthritis (OA) model as compared to sham operated animals. Conclusion: We provide novel data showing that rodent menisci display bone marrow areas with cells able to produce inflammatory mediators. Immunoexpression of inflammatory mediators in those bone marrow areas is significantly more pronounced in mice subjected to experimental OA.The study was funded by: UID/BIM/50005/2019, project funded by Fundação para a Ciência e a Tecnologia (FCT)/ Ministério da Ciência, Tecnologia e Ensino Superior (MCTES) through Fundos do Orçamento de Estado.PLOSRepositório da Universidade de LisboaRocha, Francisco Airton CastroGirão, Virgínia Claudia CarneiroNunes, Rodolfo de MeloPinto, Ana Carolina Matias DinellyVidal, BrunoFonseca, João Eurico2021-12-10T14:13:38Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/50332engPLoS One. 2019 Dec 20;14(12):e022698610.1371/journal.pone.02269861932-6203info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:54:27Zoai:repositorio.ul.pt:10451/50332Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:01:48.876114Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
title Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
spellingShingle Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
Rocha, Francisco Airton Castro
title_short Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
title_full Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
title_fullStr Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
title_full_unstemmed Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
title_sort Cell sources of inflammatory mediators present in bone marrow areas inside the meniscus
author Rocha, Francisco Airton Castro
author_facet Rocha, Francisco Airton Castro
Girão, Virgínia Claudia Carneiro
Nunes, Rodolfo de Melo
Pinto, Ana Carolina Matias Dinelly
Vidal, Bruno
Fonseca, João Eurico
author_role author
author2 Girão, Virgínia Claudia Carneiro
Nunes, Rodolfo de Melo
Pinto, Ana Carolina Matias Dinelly
Vidal, Bruno
Fonseca, João Eurico
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rocha, Francisco Airton Castro
Girão, Virgínia Claudia Carneiro
Nunes, Rodolfo de Melo
Pinto, Ana Carolina Matias Dinelly
Vidal, Bruno
Fonseca, João Eurico
description Copyright: © 2019 Rocha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
2021-12-10T14:13:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/50332
url http://hdl.handle.net/10451/50332
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS One. 2019 Dec 20;14(12):e0226986
10.1371/journal.pone.0226986
1932-6203
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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