Deflamin in Lupinus mutabilis

Detalhes bibliográficos
Autor(a) principal: Alvim, Maria do Carmo Enes Dias Castelino
Data de Publicação: 2020
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/21317
Resumo: Mestrado em Engenharia Agronómica / Instituto Superior de Agronomia. Universidade de Lisboa
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spelling Deflamin in Lupinus mutabilisAndean lupinMMP-9protease inhibitorgelatinase inhibitorHT29 cellsMestrado em Engenharia Agronómica / Instituto Superior de Agronomia. Universidade de LisboaLupinus mutabilis is a little-known species. Originating in the Andean region, its propagation worldwide is still deficient or has yet to occur, contrary to what happened to other lupin species. Despite this, within its genus, it is one of the most promising species, being even considered as a good alternative to soy, the world’s leading protein crop. The benefits of pulses in health are already well documented and studied, and many of these foods provide health benefits that go beyond high nutrient value, by containing several compounds that exhibit very interesting bioactivities. Many of these bioactivities have been discovered and studied in several pulses. Still, in L. mutabilis, these bioactivities are yet, for the most part, unknown. The aim of the present work is to assess the presence of deflamin in L. mutabilis, a small polypeptide oligomer with strong anti-inflammatory and anti-tumor potential, previously discovered in our research group in L. albus. The total protein extract of L. mutabilis seeds was tested for any inhibitory potential towards MMP-9. All eleven tested accessions showed inhibitory activity towards these metalloproteinases, with three standing out: JKI-210, JKI-295 and SBP. Through a reverse zymography performed under denaturing conditions a small polypeptide, with approximately 20 kDa, was detected. This was the first indication that deflamin could be present in the seeds of this lupin species, similarly to L. albus. Using a patented extraction method deflamin was isolated and then tested in vitro in HT29 cell migration assays. Accession JKI-210 stood out in the performed tests for having the highest inhibitory potential towards the activity of MMP-9. Field trials, performed in Tapada da Ajuda, Lisboa, showed that this accession, although being slightly less productive, adapted well to the Mediterranean near-coastal climate, suggesting it may be perfectly adequate to be sown in this area. The present work concludes that deflamin is present in L. mutabilis seeds, with significant bioactivity. This new discovery adds another valuable feature to an already promising cropISAFerreira, Ricardo BoavidaMartins, João Manuel NevesRepositório da Universidade de LisboaAlvim, Maria do Carmo Enes Dias Castelino2021-05-18T10:55:46Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/21317TID:203095731engAlvim, M.C.E.D.C. - Deflamin in Lupinus mutabilis. Lisboa: ISA, 2020, 66 p.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:50:45Zoai:www.repository.utl.pt:10400.5/21317Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:05:55.477758Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Deflamin in Lupinus mutabilis
title Deflamin in Lupinus mutabilis
spellingShingle Deflamin in Lupinus mutabilis
Alvim, Maria do Carmo Enes Dias Castelino
Andean lupin
MMP-9
protease inhibitor
gelatinase inhibitor
HT29 cells
title_short Deflamin in Lupinus mutabilis
title_full Deflamin in Lupinus mutabilis
title_fullStr Deflamin in Lupinus mutabilis
title_full_unstemmed Deflamin in Lupinus mutabilis
title_sort Deflamin in Lupinus mutabilis
author Alvim, Maria do Carmo Enes Dias Castelino
author_facet Alvim, Maria do Carmo Enes Dias Castelino
author_role author
dc.contributor.none.fl_str_mv Ferreira, Ricardo Boavida
Martins, João Manuel Neves
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Alvim, Maria do Carmo Enes Dias Castelino
dc.subject.por.fl_str_mv Andean lupin
MMP-9
protease inhibitor
gelatinase inhibitor
HT29 cells
topic Andean lupin
MMP-9
protease inhibitor
gelatinase inhibitor
HT29 cells
description Mestrado em Engenharia Agronómica / Instituto Superior de Agronomia. Universidade de Lisboa
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-05-18T10:55:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/21317
TID:203095731
url http://hdl.handle.net/10400.5/21317
identifier_str_mv TID:203095731
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alvim, M.C.E.D.C. - Deflamin in Lupinus mutabilis. Lisboa: ISA, 2020, 66 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA
publisher.none.fl_str_mv ISA
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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