Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/147423 |
Resumo: | Alzheimer's disease (AD) is the most common form of dementia worldwide, characterized by a progressive decline in a variety of cognitive and non-cognitive functions. The amyloid beta protein cascade hypothesis places the formation of amyloid beta protein aggregates on the first position in the complex pathological cascade leading to neurodegeneration, and therefore AD might be considered to be a protein-misfolding disease. The Ubiquitin Proteasome System (UPS), being the primary protein degradation mechanism with a fundamental role in the maintenance of proteostasis, has been identified as a putative therapeutic target to delay and/or to decelerate the progression of neurodegenerative disorders that are characterized by accumulated/aggregated proteins. The purpose of this study was to test if the activation of proteasome in vivo can alleviate AD pathology. Specifically by using two compounds with complementary modes of proteasome activation and documented antioxidant and redox regulating properties in the 5xFAD transgenic mice model of AD, we ameliorated a number of AD related deficits. Shortly after proteasome activation we detected significantly reduced amyloid-beta load correlated with improved motor functions, reduced anxiety and frailty level. Essentially, to our knowledge this is the first report to demonstrate a dual activation of the proteasome and its downstream effects. In conclusion, these findings open up new directions for future therapeutic potential of proteasome-mediated proteolysis enhancement. |
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Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotypeInvolvement of proteasome activationAlzheimer's diseaseBehaviorFrailtyProteasomeTherapeuticBiochemistryPhysiology (medical)Alzheimer's disease (AD) is the most common form of dementia worldwide, characterized by a progressive decline in a variety of cognitive and non-cognitive functions. The amyloid beta protein cascade hypothesis places the formation of amyloid beta protein aggregates on the first position in the complex pathological cascade leading to neurodegeneration, and therefore AD might be considered to be a protein-misfolding disease. The Ubiquitin Proteasome System (UPS), being the primary protein degradation mechanism with a fundamental role in the maintenance of proteostasis, has been identified as a putative therapeutic target to delay and/or to decelerate the progression of neurodegenerative disorders that are characterized by accumulated/aggregated proteins. The purpose of this study was to test if the activation of proteasome in vivo can alleviate AD pathology. Specifically by using two compounds with complementary modes of proteasome activation and documented antioxidant and redox regulating properties in the 5xFAD transgenic mice model of AD, we ameliorated a number of AD related deficits. Shortly after proteasome activation we detected significantly reduced amyloid-beta load correlated with improved motor functions, reduced anxiety and frailty level. Essentially, to our knowledge this is the first report to demonstrate a dual activation of the proteasome and its downstream effects. In conclusion, these findings open up new directions for future therapeutic potential of proteasome-mediated proteolysis enhancement.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNMladenovic Djordjevic, Aleksandra N.Kapetanou, MariannaLoncarevic-Vasiljkovic, NatasaTodorovic, SmiljaAthanasopoulou, SofiaJovic, MilenaPrvulovic, MilicaTaoufik, EraMatsas, RebeccaKanazir, SelmaGonos, Efstathios S.2023-01-12T22:15:26Z2021-012021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16application/pdfhttp://hdl.handle.net/10362/147423eng0891-5849PURE: 27074133https://doi.org/10.1016/j.freeradbiomed.2020.11.038info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:28:31Zoai:run.unl.pt:10362/147423Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:54.920851Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype Involvement of proteasome activation |
title |
Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype |
spellingShingle |
Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype Mladenovic Djordjevic, Aleksandra N. Alzheimer's disease Behavior Frailty Proteasome Therapeutic Biochemistry Physiology (medical) |
title_short |
Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype |
title_full |
Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype |
title_fullStr |
Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype |
title_full_unstemmed |
Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype |
title_sort |
Pharmacological intervention in a transgenic mouse model improves Alzheimer's-associated pathological phenotype |
author |
Mladenovic Djordjevic, Aleksandra N. |
author_facet |
Mladenovic Djordjevic, Aleksandra N. Kapetanou, Marianna Loncarevic-Vasiljkovic, Natasa Todorovic, Smilja Athanasopoulou, Sofia Jovic, Milena Prvulovic, Milica Taoufik, Era Matsas, Rebecca Kanazir, Selma Gonos, Efstathios S. |
author_role |
author |
author2 |
Kapetanou, Marianna Loncarevic-Vasiljkovic, Natasa Todorovic, Smilja Athanasopoulou, Sofia Jovic, Milena Prvulovic, Milica Taoufik, Era Matsas, Rebecca Kanazir, Selma Gonos, Efstathios S. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) RUN |
dc.contributor.author.fl_str_mv |
Mladenovic Djordjevic, Aleksandra N. Kapetanou, Marianna Loncarevic-Vasiljkovic, Natasa Todorovic, Smilja Athanasopoulou, Sofia Jovic, Milena Prvulovic, Milica Taoufik, Era Matsas, Rebecca Kanazir, Selma Gonos, Efstathios S. |
dc.subject.por.fl_str_mv |
Alzheimer's disease Behavior Frailty Proteasome Therapeutic Biochemistry Physiology (medical) |
topic |
Alzheimer's disease Behavior Frailty Proteasome Therapeutic Biochemistry Physiology (medical) |
description |
Alzheimer's disease (AD) is the most common form of dementia worldwide, characterized by a progressive decline in a variety of cognitive and non-cognitive functions. The amyloid beta protein cascade hypothesis places the formation of amyloid beta protein aggregates on the first position in the complex pathological cascade leading to neurodegeneration, and therefore AD might be considered to be a protein-misfolding disease. The Ubiquitin Proteasome System (UPS), being the primary protein degradation mechanism with a fundamental role in the maintenance of proteostasis, has been identified as a putative therapeutic target to delay and/or to decelerate the progression of neurodegenerative disorders that are characterized by accumulated/aggregated proteins. The purpose of this study was to test if the activation of proteasome in vivo can alleviate AD pathology. Specifically by using two compounds with complementary modes of proteasome activation and documented antioxidant and redox regulating properties in the 5xFAD transgenic mice model of AD, we ameliorated a number of AD related deficits. Shortly after proteasome activation we detected significantly reduced amyloid-beta load correlated with improved motor functions, reduced anxiety and frailty level. Essentially, to our knowledge this is the first report to demonstrate a dual activation of the proteasome and its downstream effects. In conclusion, these findings open up new directions for future therapeutic potential of proteasome-mediated proteolysis enhancement. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01 2021-01-01T00:00:00Z 2023-01-12T22:15:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/147423 |
url |
http://hdl.handle.net/10362/147423 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0891-5849 PURE: 27074133 https://doi.org/10.1016/j.freeradbiomed.2020.11.038 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16 application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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