A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/3938 |
Resumo: | Tropical fruits are a rich source of phenolic compounds which are favorable in defending the human body against damage induced by free radicals (e.g., ROS, RNS). In the current work, a high throughput analytical approach based on a simple extraction procedure (QuEChERS-dSPE) combined with high-performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry (HPLC-DAD-ESI-MSn ) was used to establish the phenolic profile of tropical fruits. The proposed method showed good linearity (r2 ≥ 0.991), precision (RSD < 8 %), as well as low limits of detection (LOD ≤ 19.7 μg/L) and quantification (LOQ ≤ 65.8 μg/ L). Thirty-four phenolic compounds were identified as belonging to different chemical groups, from which only 6 were common to all tropical fruits. Pitanga showed the highest relative phenolic concentration (99.5 mg/100 g of fruit), with the passion fruit (17.5 mg/100 g of fruit) the lowest. Flavonols were the most predominant chemical group in tropical fruits, representing 77.9, 60.1, and 55.8% of the phenolic composition of pitanga, passion fruit and mango, respectively. The data obtained allow deep and comprehensive insights into the phenolic compo sition of tropical fruits in order to explore its potential bioactive activity. Nevertheless, in vivo assays using fruit extracts will be essential to recognize their potential health-promoting properties. |
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A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruitsPhenolic profileTropical fruitsQuEChERS-dSPEHPLC–DAD–ESI–MSnMethod validation.Faculdade de Ciências Exatas e da EngenhariaCentro de Química da MadeiraTropical fruits are a rich source of phenolic compounds which are favorable in defending the human body against damage induced by free radicals (e.g., ROS, RNS). In the current work, a high throughput analytical approach based on a simple extraction procedure (QuEChERS-dSPE) combined with high-performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry (HPLC-DAD-ESI-MSn ) was used to establish the phenolic profile of tropical fruits. The proposed method showed good linearity (r2 ≥ 0.991), precision (RSD < 8 %), as well as low limits of detection (LOD ≤ 19.7 μg/L) and quantification (LOQ ≤ 65.8 μg/ L). Thirty-four phenolic compounds were identified as belonging to different chemical groups, from which only 6 were common to all tropical fruits. Pitanga showed the highest relative phenolic concentration (99.5 mg/100 g of fruit), with the passion fruit (17.5 mg/100 g of fruit) the lowest. Flavonols were the most predominant chemical group in tropical fruits, representing 77.9, 60.1, and 55.8% of the phenolic composition of pitanga, passion fruit and mango, respectively. The data obtained allow deep and comprehensive insights into the phenolic compo sition of tropical fruits in order to explore its potential bioactive activity. Nevertheless, in vivo assays using fruit extracts will be essential to recognize their potential health-promoting properties.ElsevierDigitUMaSilva, CatarinaCâmara, José S.Perestrelo, Rosa2022-01-03T14:26:03Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3938engSilva, C., Câmara, J. S., & Perestrelo, R. (2021). A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits. Journal of Food Composition and Analysis, 98, 103844. ttps://doi.org/10.1016/j.jfca.2021.10384410.1016/j.jfca.2021.103844info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-17T05:57:57Zoai:digituma.uma.pt:10400.13/3938Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:07:30.717355Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits |
title |
A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits |
spellingShingle |
A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits Silva, Catarina Phenolic profile Tropical fruits QuEChERS-dSPE HPLC–DAD–ESI–MSn Method validation . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
title_short |
A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits |
title_full |
A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits |
title_fullStr |
A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits |
title_full_unstemmed |
A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits |
title_sort |
A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits |
author |
Silva, Catarina |
author_facet |
Silva, Catarina Câmara, José S. Perestrelo, Rosa |
author_role |
author |
author2 |
Câmara, José S. Perestrelo, Rosa |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Silva, Catarina Câmara, José S. Perestrelo, Rosa |
dc.subject.por.fl_str_mv |
Phenolic profile Tropical fruits QuEChERS-dSPE HPLC–DAD–ESI–MSn Method validation . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
topic |
Phenolic profile Tropical fruits QuEChERS-dSPE HPLC–DAD–ESI–MSn Method validation . Faculdade de Ciências Exatas e da Engenharia Centro de Química da Madeira |
description |
Tropical fruits are a rich source of phenolic compounds which are favorable in defending the human body against damage induced by free radicals (e.g., ROS, RNS). In the current work, a high throughput analytical approach based on a simple extraction procedure (QuEChERS-dSPE) combined with high-performance liquid chromatography-diode array detector-electrospray ionization-mass spectrometry (HPLC-DAD-ESI-MSn ) was used to establish the phenolic profile of tropical fruits. The proposed method showed good linearity (r2 ≥ 0.991), precision (RSD < 8 %), as well as low limits of detection (LOD ≤ 19.7 μg/L) and quantification (LOQ ≤ 65.8 μg/ L). Thirty-four phenolic compounds were identified as belonging to different chemical groups, from which only 6 were common to all tropical fruits. Pitanga showed the highest relative phenolic concentration (99.5 mg/100 g of fruit), with the passion fruit (17.5 mg/100 g of fruit) the lowest. Flavonols were the most predominant chemical group in tropical fruits, representing 77.9, 60.1, and 55.8% of the phenolic composition of pitanga, passion fruit and mango, respectively. The data obtained allow deep and comprehensive insights into the phenolic compo sition of tropical fruits in order to explore its potential bioactive activity. Nevertheless, in vivo assays using fruit extracts will be essential to recognize their potential health-promoting properties. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-01-03T14:26:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/3938 |
url |
http://hdl.handle.net/10400.13/3938 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, C., Câmara, J. S., & Perestrelo, R. (2021). A high-throughput analytical strategy based on QuEChERS-dSPE/HPLC–DAD–ESI-MSn to establish the phenolic profile of tropical fruits. Journal of Food Composition and Analysis, 98, 103844. ttps://doi.org/10.1016/j.jfca.2021.103844 10.1016/j.jfca.2021.103844 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799129943760699392 |