Insights into the population structure and pan-genome of Haemophilus influenzae

Detalhes bibliográficos
Autor(a) principal: Pinto, M.
Data de Publicação: 2018
Outros Autores: González-Díaz, A., Machado, M.P., Duarte, S., Vieira, L., Carriço, J.A., Marti, S., Bajanca-Lavado, M.P., Gomes, J.P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/6267
Resumo: Supplementary data to this article can be found online at https:// doi.org/10.1016/j.meegid.2018.10.025.
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spelling Insights into the population structure and pan-genome of Haemophilus influenzaeHaemophilus InfluenzaeNon-typeablePan-genomePathogenesisWhole-genome SequencingGenomicsComputational BiologyGenetic VariationHaemophilus InfectionsHumansPhylogenyPolymorphism, Single NucleotideVirulenceGenome, BacterialInfecções RespiratóriasSupplementary data to this article can be found online at https:// doi.org/10.1016/j.meegid.2018.10.025.The human-restricted bacterium Haemophilus influenzae is responsible for respiratory infections in both children and adults. While colonization begins in the upper airways, it can spread throughout the respiratory tract potentially leading to invasive infections. Although the spread of H. influenzae serotype b (Hib) has been prevented by vaccination, the emergence of infections by other serotypes as well as by non-typeable isolates (NTHi) have been observed, prompting the need for novel prevention strategies. Here, we aimed to study the population structure of H. influenzae and to get some insights into its pan-genome. We studied 305H. influenzae strains, enrolling 217 publicly available genomes, as well as 88 newly sequenced H. influenzae invasive strains isolated in Portugal, spanning a 24-year period. NTHi isolates presented a core-SNP-based genetic diversity about 10-fold higher than the one observed for Hib. The analysis of key factors involved in pathogenesis, such as lipooligosaccharides, hemagglutinating pili and High Molecular Weight-adhesins, suggests that NTHi shape its virulence repertoire, either by acquisition and loss of genes or by SNP-based diversification, likely towards host immune evasion and persistence. Discreet NTHi subpopulations structures are proposed based on core-genome supported with 17 candidate genetic markers identified in the accessory genome. Additionally, this study provides two bioinformatics tools for in silico rapid identification of H. influenzae serotypes and NTHi clades previously proposed, obviating laboratory-based demanding procedures. The present study constitutes an important genomic framework that could lay way for future studies on the genetic determinants underlying invasiveness and disease and population structure of H. influenzae.This work was supported by GenomePT (ref. POCI-01-0145-FEDER022184) from Fundação para a Ciência e Tecnologia, Portugal.ElsevierRepositório Científico do Instituto Nacional de SaúdePinto, M.González-Díaz, A.Machado, M.P.Duarte, S.Vieira, L.Carriço, J.A.Marti, S.Bajanca-Lavado, M.P.Gomes, J.P.2019-03-21T14:48:37Z2018-10-312018-10-31T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/6267engInfect Genet Evol. 2019 Jan;67:126-135. doi: 10.1016/j.meegid.2018.10.025. Epub 2018 Oct 31.1567-134810.1016/j.meegid.2018.10.025info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:18Zoai:repositorio.insa.pt:10400.18/6267Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:40:53.576716Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Insights into the population structure and pan-genome of Haemophilus influenzae
title Insights into the population structure and pan-genome of Haemophilus influenzae
spellingShingle Insights into the population structure and pan-genome of Haemophilus influenzae
Pinto, M.
Haemophilus Influenzae
Non-typeable
Pan-genome
Pathogenesis
Whole-genome Sequencing
Genomics
Computational Biology
Genetic Variation
Haemophilus Infections
Humans
Phylogeny
Polymorphism, Single Nucleotide
Virulence
Genome, Bacterial
Infecções Respiratórias
title_short Insights into the population structure and pan-genome of Haemophilus influenzae
title_full Insights into the population structure and pan-genome of Haemophilus influenzae
title_fullStr Insights into the population structure and pan-genome of Haemophilus influenzae
title_full_unstemmed Insights into the population structure and pan-genome of Haemophilus influenzae
title_sort Insights into the population structure and pan-genome of Haemophilus influenzae
author Pinto, M.
author_facet Pinto, M.
González-Díaz, A.
Machado, M.P.
Duarte, S.
Vieira, L.
Carriço, J.A.
Marti, S.
Bajanca-Lavado, M.P.
Gomes, J.P.
author_role author
author2 González-Díaz, A.
Machado, M.P.
Duarte, S.
Vieira, L.
Carriço, J.A.
Marti, S.
Bajanca-Lavado, M.P.
Gomes, J.P.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Pinto, M.
González-Díaz, A.
Machado, M.P.
Duarte, S.
Vieira, L.
Carriço, J.A.
Marti, S.
Bajanca-Lavado, M.P.
Gomes, J.P.
dc.subject.por.fl_str_mv Haemophilus Influenzae
Non-typeable
Pan-genome
Pathogenesis
Whole-genome Sequencing
Genomics
Computational Biology
Genetic Variation
Haemophilus Infections
Humans
Phylogeny
Polymorphism, Single Nucleotide
Virulence
Genome, Bacterial
Infecções Respiratórias
topic Haemophilus Influenzae
Non-typeable
Pan-genome
Pathogenesis
Whole-genome Sequencing
Genomics
Computational Biology
Genetic Variation
Haemophilus Infections
Humans
Phylogeny
Polymorphism, Single Nucleotide
Virulence
Genome, Bacterial
Infecções Respiratórias
description Supplementary data to this article can be found online at https:// doi.org/10.1016/j.meegid.2018.10.025.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-31
2018-10-31T00:00:00Z
2019-03-21T14:48:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/6267
url http://hdl.handle.net/10400.18/6267
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Infect Genet Evol. 2019 Jan;67:126-135. doi: 10.1016/j.meegid.2018.10.025. Epub 2018 Oct 31.
1567-1348
10.1016/j.meegid.2018.10.025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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