Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/24781 |
Resumo: | Research Areas: Agriculture; Veterinary Sciences |
id |
RCAP_f94483d045ef661883cf6d81d4047b4f |
---|---|
oai_identifier_str |
oai:www.repository.utl.pt:10400.5/24781 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyasesMacroalgaUlva lactucaCarbohydrate-active enzymeUlvan lyaseCell wall degradationResearch Areas: Agriculture; Veterinary SciencesABSTRACT - Green macroalgae, e.g., Ulva lactuca, are valuable bioactive sources of nutrients; but algae recalcitrant cell walls, composed of a complex cross-linked matrix of polysaccharides, can compromise their utilization as feedstuffs for monogastric animals. This study aimed to evaluate the ability of pre-selected Carbohy- drate-Active enZymes (CAZymes) and sulfatases to degrade U. lactuca cell walls and release nutritive compounds. A databank of 199 recombinant CAZymes and sulfatases was tested in vitro for their action towards U. lactuca cell wall polysaccharides. The enzymes were incubated with the macroalga, either alone or in combination, to release reducing sugars and decrease fluorescence intensity of Calcofluor White stained cell walls. The individual action of a polysaccharide lyase family 25 (PL25), an ulvan lyase, was shown to be the most efficient in cell wall disruption. The ulvan lyase treatment, in triplicate measures, promoted the release of 4.54 g/L (P < 0.001) reducing sugars, a mono- and oligosaccharides release of 11.4 and 11.2 mmol/100 g of dried alga (P < 0.01), respectively, and a decrease of 41.7% (P < 0.001) in cell wall fluorescence, in comparison to control. The ability of ulvan lyase treatment to promote the release of nutritional compounds from alga biomass was also evaluated. A release of some monounsaturated fatty acids was observed, particularly the health beneficial 18:1c9 (P < 0.001). How- ever, no significant release of total fatty acids (P > 0.05), proteins (P ¼ 0.861) or pigments (P > 0.05) was found. These results highlight the capacity of a single recombinant ulvan lyase (PL25 family) to incompletely disrupt U. lactuca cell walls. This enzyme could enhance the bioaccessibility of U. lactuca bioactive products with promising utilization in the feed industry.KEAI PUBLISHINGRepositório da Universidade de LisboaCosta, MonicaPio, Luís BernardoBule, PedroDuarte, MarleneAlfaia, CristinaCoelho, DiogoBras, JoanaFontes, Carlos M.G.A.Prates, José A.MCardoso, Vânia2022-07-07T17:10:33Z2022-062022-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24781engCosta MM, Pio LB, Bule P, Cardoso VA, Duarte M, Alfaia CM, Coelho DF, Bras JA, Fontes CMGA, Prates JAM . 2022. Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases. Animal Nutrition, 9:p.184-192. DOI: https://doi.org/10.1016/j.aninu.2022.01.0042405-6383https://doi.org/10.1016/j.aninu.2022.01.0042405-6545info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:54:27Zoai:www.repository.utl.pt:10400.5/24781Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:44.978164Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases |
title |
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases |
spellingShingle |
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases Costa, Monica Macroalga Ulva lactuca Carbohydrate-active enzyme Ulvan lyase Cell wall degradation |
title_short |
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases |
title_full |
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases |
title_fullStr |
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases |
title_full_unstemmed |
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases |
title_sort |
Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases |
author |
Costa, Monica |
author_facet |
Costa, Monica Pio, Luís Bernardo Bule, Pedro Duarte, Marlene Alfaia, Cristina Coelho, Diogo Bras, Joana Fontes, Carlos M.G.A. Prates, José A.M Cardoso, Vânia |
author_role |
author |
author2 |
Pio, Luís Bernardo Bule, Pedro Duarte, Marlene Alfaia, Cristina Coelho, Diogo Bras, Joana Fontes, Carlos M.G.A. Prates, José A.M Cardoso, Vânia |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Costa, Monica Pio, Luís Bernardo Bule, Pedro Duarte, Marlene Alfaia, Cristina Coelho, Diogo Bras, Joana Fontes, Carlos M.G.A. Prates, José A.M Cardoso, Vânia |
dc.subject.por.fl_str_mv |
Macroalga Ulva lactuca Carbohydrate-active enzyme Ulvan lyase Cell wall degradation |
topic |
Macroalga Ulva lactuca Carbohydrate-active enzyme Ulvan lyase Cell wall degradation |
description |
Research Areas: Agriculture; Veterinary Sciences |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-07T17:10:33Z 2022-06 2022-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/24781 |
url |
http://hdl.handle.net/10400.5/24781 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Costa MM, Pio LB, Bule P, Cardoso VA, Duarte M, Alfaia CM, Coelho DF, Bras JA, Fontes CMGA, Prates JAM . 2022. Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases. Animal Nutrition, 9:p.184-192. DOI: https://doi.org/10.1016/j.aninu.2022.01.004 2405-6383 https://doi.org/10.1016/j.aninu.2022.01.004 2405-6545 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
KEAI PUBLISHING |
publisher.none.fl_str_mv |
KEAI PUBLISHING |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131182236958720 |