Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/1911 |
Resumo: | Aim: The uninephrectomised and three-quarter nephrectomised (3/4nx) rats present dopamine-sensitive enhanced natriuresis. This is accompanied in uninephrectomised rats by a reduced jejunal Na+,K+-ATPase activity with recovered sensitivity to inhibition by dopamine. The present study examined the jejunal Na+,K+-ATPase activity and the role of dopamine in 3/4nx animals. Methods: Fourteen days after surgery, the L-amino acid decarboxylase activity (AADC) activity, the enzyme responsible for the synthesis of dopamine, and the Na+,K+-ATPase activity, were determined in jejunal epithelial cells from 3/4nx and Sham rats. In addition, the effect of dopamine (1 mu mol/L) on jejunal Na+,K+-ATPase activity was evaluated in both groups. Results: The 3/4nx rats presented a reduced AADC activity in jejunal epithelial cells (V-max in nmol/mg prot/15 min, 142 +/- 6 vs 190 +/- 10, P < 0.05). In addition, the jejunal Na+,K+-ATPase activity was increased in 3/4nx rats (Pi release in nmol/mg prot/min, 137 +/- 1 vs 122 +/- 2, P < 0.05). However, dopamine was unable to inhibit the Na+,K+-ATPase activity in jejunal epithelial cells from both 3/4nx and Sham animals. Conclusions: In contrast to uninephrectomy, the jejunal Na+,K+-ATPase activity is increased in 3/4nx rats and is not sensitive to inhibition by dopamine. |
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Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiencyCiências da SaúdeHealth sciencesAim: The uninephrectomised and three-quarter nephrectomised (3/4nx) rats present dopamine-sensitive enhanced natriuresis. This is accompanied in uninephrectomised rats by a reduced jejunal Na+,K+-ATPase activity with recovered sensitivity to inhibition by dopamine. The present study examined the jejunal Na+,K+-ATPase activity and the role of dopamine in 3/4nx animals. Methods: Fourteen days after surgery, the L-amino acid decarboxylase activity (AADC) activity, the enzyme responsible for the synthesis of dopamine, and the Na+,K+-ATPase activity, were determined in jejunal epithelial cells from 3/4nx and Sham rats. In addition, the effect of dopamine (1 mu mol/L) on jejunal Na+,K+-ATPase activity was evaluated in both groups. Results: The 3/4nx rats presented a reduced AADC activity in jejunal epithelial cells (V-max in nmol/mg prot/15 min, 142 +/- 6 vs 190 +/- 10, P < 0.05). In addition, the jejunal Na+,K+-ATPase activity was increased in 3/4nx rats (Pi release in nmol/mg prot/min, 137 +/- 1 vs 122 +/- 2, P < 0.05). However, dopamine was unable to inhibit the Na+,K+-ATPase activity in jejunal epithelial cells from both 3/4nx and Sham animals. Conclusions: In contrast to uninephrectomy, the jejunal Na+,K+-ATPase activity is increased in 3/4nx rats and is not sensitive to inhibition by dopamine.20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/1911eng1320-535810.1111/j.1440-1797.2006.00533.xMaria MaiaPaula SerrãoMaria Manuela MouraManuel Pestanainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:40:59Zoai:repositorio-aberto.up.pt:10216/1911Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:45:34.700404Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency |
title |
Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency |
spellingShingle |
Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency Maria Maia Ciências da Saúde Health sciences |
title_short |
Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency |
title_full |
Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency |
title_fullStr |
Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency |
title_full_unstemmed |
Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency |
title_sort |
Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency |
author |
Maria Maia |
author_facet |
Maria Maia Paula Serrão Maria Manuela Moura Manuel Pestana |
author_role |
author |
author2 |
Paula Serrão Maria Manuela Moura Manuel Pestana |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Maria Maia Paula Serrão Maria Manuela Moura Manuel Pestana |
dc.subject.por.fl_str_mv |
Ciências da Saúde Health sciences |
topic |
Ciências da Saúde Health sciences |
description |
Aim: The uninephrectomised and three-quarter nephrectomised (3/4nx) rats present dopamine-sensitive enhanced natriuresis. This is accompanied in uninephrectomised rats by a reduced jejunal Na+,K+-ATPase activity with recovered sensitivity to inhibition by dopamine. The present study examined the jejunal Na+,K+-ATPase activity and the role of dopamine in 3/4nx animals. Methods: Fourteen days after surgery, the L-amino acid decarboxylase activity (AADC) activity, the enzyme responsible for the synthesis of dopamine, and the Na+,K+-ATPase activity, were determined in jejunal epithelial cells from 3/4nx and Sham rats. In addition, the effect of dopamine (1 mu mol/L) on jejunal Na+,K+-ATPase activity was evaluated in both groups. Results: The 3/4nx rats presented a reduced AADC activity in jejunal epithelial cells (V-max in nmol/mg prot/15 min, 142 +/- 6 vs 190 +/- 10, P < 0.05). In addition, the jejunal Na+,K+-ATPase activity was increased in 3/4nx rats (Pi release in nmol/mg prot/min, 137 +/- 1 vs 122 +/- 2, P < 0.05). However, dopamine was unable to inhibit the Na+,K+-ATPase activity in jejunal epithelial cells from both 3/4nx and Sham animals. Conclusions: In contrast to uninephrectomy, the jejunal Na+,K+-ATPase activity is increased in 3/4nx rats and is not sensitive to inhibition by dopamine. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006 2006-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/1911 |
url |
https://hdl.handle.net/10216/1911 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1320-5358 10.1111/j.1440-1797.2006.00533.x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135772940435457 |