New sensing materials of molecularly-imprinted polymers for the selective recognition of Chlortetracycline

Detalhes bibliográficos
Autor(a) principal: Guerreiro, J. Rafaela L.
Data de Publicação: 2011
Outros Autores: Freitas, Victor, Sales, M. Goreti F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/3055
Resumo: As a result of the stressful conditions in aquaculture facilities there is a high risk of bacterial infections among cultured fish. Chlortetracycline (CTC) is one of the antimicrobials used to solve this problem. It is a broad spectrum antibacterial active against a wide range of Gram-positive and Gram-negative bacteria. Numerous analytical methods for screening, identifying, and quantifying CTC in animal products have been developed over the years. An alternative and advantageous method should rely on expeditious and efficient procedures providing highly specific and sensitive measurements in food samples. Ion-selective electrodes (ISEs) could meet these criteria. The only ISE reported in literature for this purpose used traditional electro-active materials. A selectivity enhancement could however be achieved after improving the analyte recognition by molecularly imprinted polymers (MIPs). Several MIP particles were synthesized and used as electro-active materials. ISEs based in methacrylic acid monomers showed the best analytical performance according to slope (62.5 and 68.6 mV/decade) and detection limit (4.1×10−5 and 5.5×10−5 mol L−1). The electrodes displayed good selectivity. The ISEs are not affected by pH changes ranging from 2.5 to 13. The sensors were successfully applied to the analysis of serum, urine and fish samples.
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spelling New sensing materials of molecularly-imprinted polymers for the selective recognition of ChlortetracyclineChlortetracyclineMolecularly-imprinted sensorsPotentiometryFIAFishAs a result of the stressful conditions in aquaculture facilities there is a high risk of bacterial infections among cultured fish. Chlortetracycline (CTC) is one of the antimicrobials used to solve this problem. It is a broad spectrum antibacterial active against a wide range of Gram-positive and Gram-negative bacteria. Numerous analytical methods for screening, identifying, and quantifying CTC in animal products have been developed over the years. An alternative and advantageous method should rely on expeditious and efficient procedures providing highly specific and sensitive measurements in food samples. Ion-selective electrodes (ISEs) could meet these criteria. The only ISE reported in literature for this purpose used traditional electro-active materials. A selectivity enhancement could however be achieved after improving the analyte recognition by molecularly imprinted polymers (MIPs). Several MIP particles were synthesized and used as electro-active materials. ISEs based in methacrylic acid monomers showed the best analytical performance according to slope (62.5 and 68.6 mV/decade) and detection limit (4.1×10−5 and 5.5×10−5 mol L−1). The electrodes displayed good selectivity. The ISEs are not affected by pH changes ranging from 2.5 to 13. The sensors were successfully applied to the analysis of serum, urine and fish samples.ElsevierRepositório Científico do Instituto Politécnico do PortoGuerreiro, J. Rafaela L.Freitas, VictorSales, M. Goreti F.2013-11-29T16:35:48Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/3055eng0026-265X10.1016/j.microc.2010.08.011metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:42:47Zoai:recipp.ipp.pt:10400.22/3055Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:24:03.759140Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv New sensing materials of molecularly-imprinted polymers for the selective recognition of Chlortetracycline
title New sensing materials of molecularly-imprinted polymers for the selective recognition of Chlortetracycline
spellingShingle New sensing materials of molecularly-imprinted polymers for the selective recognition of Chlortetracycline
Guerreiro, J. Rafaela L.
Chlortetracycline
Molecularly-imprinted sensors
Potentiometry
FIA
Fish
title_short New sensing materials of molecularly-imprinted polymers for the selective recognition of Chlortetracycline
title_full New sensing materials of molecularly-imprinted polymers for the selective recognition of Chlortetracycline
title_fullStr New sensing materials of molecularly-imprinted polymers for the selective recognition of Chlortetracycline
title_full_unstemmed New sensing materials of molecularly-imprinted polymers for the selective recognition of Chlortetracycline
title_sort New sensing materials of molecularly-imprinted polymers for the selective recognition of Chlortetracycline
author Guerreiro, J. Rafaela L.
author_facet Guerreiro, J. Rafaela L.
Freitas, Victor
Sales, M. Goreti F.
author_role author
author2 Freitas, Victor
Sales, M. Goreti F.
author2_role author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Guerreiro, J. Rafaela L.
Freitas, Victor
Sales, M. Goreti F.
dc.subject.por.fl_str_mv Chlortetracycline
Molecularly-imprinted sensors
Potentiometry
FIA
Fish
topic Chlortetracycline
Molecularly-imprinted sensors
Potentiometry
FIA
Fish
description As a result of the stressful conditions in aquaculture facilities there is a high risk of bacterial infections among cultured fish. Chlortetracycline (CTC) is one of the antimicrobials used to solve this problem. It is a broad spectrum antibacterial active against a wide range of Gram-positive and Gram-negative bacteria. Numerous analytical methods for screening, identifying, and quantifying CTC in animal products have been developed over the years. An alternative and advantageous method should rely on expeditious and efficient procedures providing highly specific and sensitive measurements in food samples. Ion-selective electrodes (ISEs) could meet these criteria. The only ISE reported in literature for this purpose used traditional electro-active materials. A selectivity enhancement could however be achieved after improving the analyte recognition by molecularly imprinted polymers (MIPs). Several MIP particles were synthesized and used as electro-active materials. ISEs based in methacrylic acid monomers showed the best analytical performance according to slope (62.5 and 68.6 mV/decade) and detection limit (4.1×10−5 and 5.5×10−5 mol L−1). The electrodes displayed good selectivity. The ISEs are not affected by pH changes ranging from 2.5 to 13. The sensors were successfully applied to the analysis of serum, urine and fish samples.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2013-11-29T16:35:48Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/3055
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0026-265X
10.1016/j.microc.2010.08.011
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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