A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors

Detalhes bibliográficos
Autor(a) principal: Pires, Diana P.
Data de Publicação: 2017
Outros Autores: Dötsch, Andreas, Anderson, Erin M., Hao, Youai, Khursigara, Cezar M., Lam, J. S., Sillankorva, Sanna, Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/47422
Resumo: The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fmicb. 2017.01229/full#supplementary-material
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spelling A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptorsBiofilmsBacteriophagesP. aeruginosaBacterial resistancephage infectionScience & TechnologyThe Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fmicb. 2017.01229/full#supplementary-materialAntibiotic resistance constitutes one of the most serious threats to the global public health and urgently requires new and effective solutions. Bacteriophages are bacterial viruses increasingly recognized as being good alternatives to traditional antibiotic therapies. In this study, the efficacy of phages, targeting different cell receptors, against Pseudomonas aeruginosa PAO1 biofilm and planktonic cell cultures was evaluated over the course of 48h. Although significant reductions in the number of viable cells were achieved for both cases, the high level of adaptability of the bacteria in response to the selective pressure caused by phage treatment resulted in the emergence of phage-resistant variants. To further investigate the genetic makeup of phage-resistant variants isolated from biofilm infection experiments, some of these bacteria were selected for phenotypic and genotypic characterization. Whole genome sequencing was performed on five phage-resistant variants and all of them carried mutations affecting the galU gene as well as one of pil genes. The sequencing analysis further revealed that three of the P. aeruginosa PAO1 variants carry large deletions (> 200 kbp) in their genomes. Complementation of the galU mutants with wild-type galU in trans restored LPS expression on the bacterial cell surface of these bacterial strains and rendered the complemented strains to be sensitive to phages. This provides unequivocal evidence that inactivation of galU function was associated with resistance to the phages that uses LPS as primary receptors. Overall, this work demonstrates that P. aeruginosa biofilms can survive phage attack and develop phage-resistant variants exhibiting defective LPS production and loss of type IV pili that are well adapted to the biofilm mode of growth.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the project PTDC/BBB-BSS/6471/2014; the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER006684) and BioTecNorte operation (NORTE-01-0145-FEDER000004)funded by European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. SS is an FCT investigator (IF/01413/2013). Work done in the lab of JL is supported by Canadian Institutes of Health Research (Grant MOP-14687). YH was a recipient of a postdoctoral fellowship award from Cystic Fibrosis, and JL holds a Canada Research Chair in Cystic Fibrosis and Microbial Glycobiology funded by the Canadian Foundation of Innovation. Work done in the lab of CK is supported by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada(#371639).info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoPires, Diana P.Dötsch, AndreasAnderson, Erin M.Hao, YouaiKhursigara, Cezar M.Lam, J. S.Sillankorva, SannaAzeredo, Joana20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/47422engPires, Diana P.; Dötsch, Andreas; Anderson, Erin M.; Hao, Youai; Khursigara, Cezar M.; Lam, J. S.; Sillankorva, Sanna; Azeredo, Joana, A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors. Frontiers in Microbiology, 8(1229), 20171664-302X1664-302X10.3389/fmicb.2017.01229http://journal.frontiersin.org/journal/microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:46:26Zoai:repositorium.sdum.uminho.pt:1822/47422Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:44:26.784786Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors
title A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors
spellingShingle A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors
Pires, Diana P.
Biofilms
Bacteriophages
P. aeruginosa
Bacterial resistance
phage infection
Science & Technology
title_short A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors
title_full A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors
title_fullStr A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors
title_full_unstemmed A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors
title_sort A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors
author Pires, Diana P.
author_facet Pires, Diana P.
Dötsch, Andreas
Anderson, Erin M.
Hao, Youai
Khursigara, Cezar M.
Lam, J. S.
Sillankorva, Sanna
Azeredo, Joana
author_role author
author2 Dötsch, Andreas
Anderson, Erin M.
Hao, Youai
Khursigara, Cezar M.
Lam, J. S.
Sillankorva, Sanna
Azeredo, Joana
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pires, Diana P.
Dötsch, Andreas
Anderson, Erin M.
Hao, Youai
Khursigara, Cezar M.
Lam, J. S.
Sillankorva, Sanna
Azeredo, Joana
dc.subject.por.fl_str_mv Biofilms
Bacteriophages
P. aeruginosa
Bacterial resistance
phage infection
Science & Technology
topic Biofilms
Bacteriophages
P. aeruginosa
Bacterial resistance
phage infection
Science & Technology
description The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fmicb. 2017.01229/full#supplementary-material
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/47422
url http://hdl.handle.net/1822/47422
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pires, Diana P.; Dötsch, Andreas; Anderson, Erin M.; Hao, Youai; Khursigara, Cezar M.; Lam, J. S.; Sillankorva, Sanna; Azeredo, Joana, A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors. Frontiers in Microbiology, 8(1229), 2017
1664-302X
1664-302X
10.3389/fmicb.2017.01229
http://journal.frontiersin.org/journal/microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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