Spectrophotometric determination of carboxyhaemoglobin in a sample of automobile mechanics occupationally exposed to carbon monoxide

• Autor(a) principal: Dias, Catarina
• Data de Publicação: 2018
• Outros Autores: Marques, Margarida, Santos, Vanessa, Tavares, Ana Sofia
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.8/4508
• topic: Carboxyhaemoglobin; Mechanics; Carbon monoxide; Occupational exposure

Introduction: Carbon monoxide (CO) is a toxic, colourless gas that results from the incomplete combustion of coal and other petroleum-derived materials. Within anthropogenic sources of carbon monoxide emissions, air pollution from car emissions accounts for about 75% of the total air pollution present in atmosphere. Inhalation of this gas is considered to be potentially toxic to the body, resulting in a haemoglobin variant with reduced oxygen transport capacity, carboxyhaemoglobin (COHb). As the endogenous concentration of COHb in a healthy adult varies from 0.1% to 1.0%, higher values can lead to respiratory problems, visual perception impairment and manual dexterity, headache and nausea. The present study aimed to compare the amount of carboxyhaemoglobin present in a sample of 8 mechanics, working at automobile repair shops (test group), with the carboxyhaemoglobin present in a control group (16 non-mechanic individuals), via a spectrophotometric method, as well as to verify if the years of labour activity, along with the use of personal protective equipment (PPE) influences this parameter. Methods: A simple descriptive level II research study was developed to find relationships between variables. It is considered to be a case-control study and is further classified as analytical, observational, transversal and retrospective. Results: The results showed that the blood concentration of COHb (%COHb) in the test group was on average 0.653 ± 0.087% and in the control group, it was on average 0.477 ± 0.133%. Statistically significant differences were observed in relation to the carboxyhaemoglobin values between the test group and the control group (p = 0.002); however, no statistically significant differences were found between the years of work activity (p = 0.711) and use of PPE (p = 0.392) when compared to the carboxyhaemoglobin values of the test group. Although statistically significant differences were obtained in COHb values between the two groups, values higher than 1.0% in the test group (average of 0.653%) were not obtained. Conclusions: In this study, although no altered COHb values were found in the test group, new lines of research on this subject are recommended, aiming to broaden and deepen the field of knowledge, aiming at the protection of this type of workers.