The unique biosynthetic route from Lupinus Beta-conglutin gene to blad
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/17785 |
Resumo: | Background: During seed germination, b-conglutin undergoes a major cycle of limited proteolysis in which many of its constituent subunits are processed into a 20 kDa polypeptide termed blad. Blad is the main component of a glycooligomer, accumulating exclusively in the cotyledons of Lupinus species, between days 4 and 12 after the onset of germination. Principal Findings: The sequence of the gene encoding b-conglutin precursor (1791 nucleotides) is reported. This gene, which shares 44 to 57% similarity and 20 to 37% identity with other vicilin-like protein genes, includes several features in common with these globulins, but also specific hallmarks. Most notable is the presence of an ubiquitin interacting motif (UIM), which possibly links the unique catabolic route of b-conglutin to the ubiquitin/proteasome proteolytic pathway. Significance: Blad forms through a unique route from and is a stable intermediary product of its precursor, b-conglutin, the major Lupinus seed storage protein. It is composed of 173 amino acid residues, is encoded by an intron-containing, internal fragment of the gene that codes for b-conglutin precursor (nucleotides 394 to 913) and exhibits an isoelectric point of 9.6 and a molecular mass of 20,404.85 Da. Consistent with its role as a storage protein, blad contains an extremely high proportion of the nitrogen-rich amino acids |
id |
RCAP_fb32b57c603c05459a28fba66f038879 |
---|---|
oai_identifier_str |
oai:www.repository.utl.pt:10400.5/17785 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
The unique biosynthetic route from Lupinus Beta-conglutin gene to bladLupinusbladBackground: During seed germination, b-conglutin undergoes a major cycle of limited proteolysis in which many of its constituent subunits are processed into a 20 kDa polypeptide termed blad. Blad is the main component of a glycooligomer, accumulating exclusively in the cotyledons of Lupinus species, between days 4 and 12 after the onset of germination. Principal Findings: The sequence of the gene encoding b-conglutin precursor (1791 nucleotides) is reported. This gene, which shares 44 to 57% similarity and 20 to 37% identity with other vicilin-like protein genes, includes several features in common with these globulins, but also specific hallmarks. Most notable is the presence of an ubiquitin interacting motif (UIM), which possibly links the unique catabolic route of b-conglutin to the ubiquitin/proteasome proteolytic pathway. Significance: Blad forms through a unique route from and is a stable intermediary product of its precursor, b-conglutin, the major Lupinus seed storage protein. It is composed of 173 amino acid residues, is encoded by an intron-containing, internal fragment of the gene that codes for b-conglutin precursor (nucleotides 394 to 913) and exhibits an isoelectric point of 9.6 and a molecular mass of 20,404.85 Da. Consistent with its role as a storage protein, blad contains an extremely high proportion of the nitrogen-rich amino acidsPlos ONERepositório da Universidade de LisboaMonteiro, SaraFreitas, ReginaRajasekhar, Baru T.Teixeira, Artur R.Ferreira, Ricardo Boavida2019-05-09T13:44:25Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/17785engMonteiro S, Freitas R, Rajasekhar BT, Teixeira AR, Ferreira RB (2010) The Unique Biosynthetic Route from Lupinus b-Conglutin Gene to Blad. PLoS ONE 5(1): e854210.1371/journal.pone.0008542info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:47:26Zoai:www.repository.utl.pt:10400.5/17785Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:02:57.445303Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The unique biosynthetic route from Lupinus Beta-conglutin gene to blad |
title |
The unique biosynthetic route from Lupinus Beta-conglutin gene to blad |
spellingShingle |
The unique biosynthetic route from Lupinus Beta-conglutin gene to blad Monteiro, Sara Lupinus blad |
title_short |
The unique biosynthetic route from Lupinus Beta-conglutin gene to blad |
title_full |
The unique biosynthetic route from Lupinus Beta-conglutin gene to blad |
title_fullStr |
The unique biosynthetic route from Lupinus Beta-conglutin gene to blad |
title_full_unstemmed |
The unique biosynthetic route from Lupinus Beta-conglutin gene to blad |
title_sort |
The unique biosynthetic route from Lupinus Beta-conglutin gene to blad |
author |
Monteiro, Sara |
author_facet |
Monteiro, Sara Freitas, Regina Rajasekhar, Baru T. Teixeira, Artur R. Ferreira, Ricardo Boavida |
author_role |
author |
author2 |
Freitas, Regina Rajasekhar, Baru T. Teixeira, Artur R. Ferreira, Ricardo Boavida |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Monteiro, Sara Freitas, Regina Rajasekhar, Baru T. Teixeira, Artur R. Ferreira, Ricardo Boavida |
dc.subject.por.fl_str_mv |
Lupinus blad |
topic |
Lupinus blad |
description |
Background: During seed germination, b-conglutin undergoes a major cycle of limited proteolysis in which many of its constituent subunits are processed into a 20 kDa polypeptide termed blad. Blad is the main component of a glycooligomer, accumulating exclusively in the cotyledons of Lupinus species, between days 4 and 12 after the onset of germination. Principal Findings: The sequence of the gene encoding b-conglutin precursor (1791 nucleotides) is reported. This gene, which shares 44 to 57% similarity and 20 to 37% identity with other vicilin-like protein genes, includes several features in common with these globulins, but also specific hallmarks. Most notable is the presence of an ubiquitin interacting motif (UIM), which possibly links the unique catabolic route of b-conglutin to the ubiquitin/proteasome proteolytic pathway. Significance: Blad forms through a unique route from and is a stable intermediary product of its precursor, b-conglutin, the major Lupinus seed storage protein. It is composed of 173 amino acid residues, is encoded by an intron-containing, internal fragment of the gene that codes for b-conglutin precursor (nucleotides 394 to 913) and exhibits an isoelectric point of 9.6 and a molecular mass of 20,404.85 Da. Consistent with its role as a storage protein, blad contains an extremely high proportion of the nitrogen-rich amino acids |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z 2019-05-09T13:44:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/17785 |
url |
http://hdl.handle.net/10400.5/17785 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Monteiro S, Freitas R, Rajasekhar BT, Teixeira AR, Ferreira RB (2010) The Unique Biosynthetic Route from Lupinus b-Conglutin Gene to Blad. PLoS ONE 5(1): e8542 10.1371/journal.pone.0008542 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Plos ONE |
publisher.none.fl_str_mv |
Plos ONE |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131119660040192 |