Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/108912 https://doi.org/10.1038/srep39007 |
Resumo: | The pinewood nematode, Bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi colonizing the trees. Bursaphelenchus mucronatus, the closest related species, differs from B. xylophilus on its pathogenicity, making this nematode a good candidate for comparative analyses. Secretome profiles of B. xylophilus and B. mucronatus were obtained and proteomic differences were evaluated by quantitative SWATH-MS. From the 681 proteins initially identified, 422 were quantified and compared between B. xylophilus and B. mucronatus secretomes and from these, 243 proteins were found differentially regulated: 158 and 85 proteins were increased in B. xylophilus and B. mucronatus secretomes, respectively. While increased proteins in B. xylophilus secretome revealed a strong enrichment in proteins with peptidase activity, the increased proteins in B. mucronatus secretome were mainly related to oxidative stress responses. The changes in peptidases were evaluated at the transcription level by RT-qPCR, revealing a correlation between the mRNA levels of four cysteine peptidases with secretion levels. The analysis presented expands our knowledge about molecular basis of B. xylophilus and B. mucronatus hosts interaction and supports the hypothesis of a key role of secreted peptidases in B. xylophilus pathogenicity. |
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Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysisAnimalsGene OntologyNematodaOxidative StressProteomicsTranscriptomeThe pinewood nematode, Bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi colonizing the trees. Bursaphelenchus mucronatus, the closest related species, differs from B. xylophilus on its pathogenicity, making this nematode a good candidate for comparative analyses. Secretome profiles of B. xylophilus and B. mucronatus were obtained and proteomic differences were evaluated by quantitative SWATH-MS. From the 681 proteins initially identified, 422 were quantified and compared between B. xylophilus and B. mucronatus secretomes and from these, 243 proteins were found differentially regulated: 158 and 85 proteins were increased in B. xylophilus and B. mucronatus secretomes, respectively. While increased proteins in B. xylophilus secretome revealed a strong enrichment in proteins with peptidase activity, the increased proteins in B. mucronatus secretome were mainly related to oxidative stress responses. The changes in peptidases were evaluated at the transcription level by RT-qPCR, revealing a correlation between the mRNA levels of four cysteine peptidases with secretion levels. The analysis presented expands our knowledge about molecular basis of B. xylophilus and B. mucronatus hosts interaction and supports the hypothesis of a key role of secreted peptidases in B. xylophilus pathogenicity.2016-12-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108912http://hdl.handle.net/10316/108912https://doi.org/10.1038/srep39007eng2045-2322Cardoso, Joana M. S.Anjo, Sandra I.Fonseca, LuísEgas, ConceiçãoManadas, BrunoAbrantes, Isabelinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-25T08:41:51Zoai:estudogeral.uc.pt:10316/108912Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:08.966258Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis |
title |
Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis |
spellingShingle |
Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis Cardoso, Joana M. S. Animals Gene Ontology Nematoda Oxidative Stress Proteomics Transcriptome |
title_short |
Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis |
title_full |
Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis |
title_fullStr |
Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis |
title_full_unstemmed |
Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis |
title_sort |
Bursaphelenchus xylophilus and B. mucronatus secretomes: a comparative proteomic analysis |
author |
Cardoso, Joana M. S. |
author_facet |
Cardoso, Joana M. S. Anjo, Sandra I. Fonseca, Luís Egas, Conceição Manadas, Bruno Abrantes, Isabel |
author_role |
author |
author2 |
Anjo, Sandra I. Fonseca, Luís Egas, Conceição Manadas, Bruno Abrantes, Isabel |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cardoso, Joana M. S. Anjo, Sandra I. Fonseca, Luís Egas, Conceição Manadas, Bruno Abrantes, Isabel |
dc.subject.por.fl_str_mv |
Animals Gene Ontology Nematoda Oxidative Stress Proteomics Transcriptome |
topic |
Animals Gene Ontology Nematoda Oxidative Stress Proteomics Transcriptome |
description |
The pinewood nematode, Bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi colonizing the trees. Bursaphelenchus mucronatus, the closest related species, differs from B. xylophilus on its pathogenicity, making this nematode a good candidate for comparative analyses. Secretome profiles of B. xylophilus and B. mucronatus were obtained and proteomic differences were evaluated by quantitative SWATH-MS. From the 681 proteins initially identified, 422 were quantified and compared between B. xylophilus and B. mucronatus secretomes and from these, 243 proteins were found differentially regulated: 158 and 85 proteins were increased in B. xylophilus and B. mucronatus secretomes, respectively. While increased proteins in B. xylophilus secretome revealed a strong enrichment in proteins with peptidase activity, the increased proteins in B. mucronatus secretome were mainly related to oxidative stress responses. The changes in peptidases were evaluated at the transcription level by RT-qPCR, revealing a correlation between the mRNA levels of four cysteine peptidases with secretion levels. The analysis presented expands our knowledge about molecular basis of B. xylophilus and B. mucronatus hosts interaction and supports the hypothesis of a key role of secreted peptidases in B. xylophilus pathogenicity. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-12 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/108912 http://hdl.handle.net/10316/108912 https://doi.org/10.1038/srep39007 |
url |
http://hdl.handle.net/10316/108912 https://doi.org/10.1038/srep39007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2045-2322 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134134454452224 |