BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia

Detalhes bibliográficos
Autor(a) principal: Cordeiro, M.
Data de Publicação: 2016
Outros Autores: Giestas, L., Lima, J. C., Baptista, P. M. V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/41422
Resumo: This work was supported by Fundacao para a Ciencia e Tecnologia, Ministry of Science and Education (FCT/MEC) [PTDC/QUI-QUI/112597/2009; PTDC/BBB-NAN/1812/2012; CIGMH (PEST-OE/SAU/UI0009/2011); SFRH/BD/87836/2012 to M.C. and SFRH/BPD/88322/2012 to L.G.]; and by Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO [(UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728)].
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spelling BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemiaBiosensing TechniquesFluorescence Resonance Energy TransferFusion Proteins, bcr-ablGene Expression Regulation, LeukemicGoldHumansLeukemia, Myelogenous, Chronic, BCR-ABL PositiveMetal NanoparticlesNucleic Acid HybridizationJournal ArticleSDG 3 - Good Health and Well-beingThis work was supported by Fundacao para a Ciencia e Tecnologia, Ministry of Science and Education (FCT/MEC) [PTDC/QUI-QUI/112597/2009; PTDC/BBB-NAN/1812/2012; CIGMH (PEST-OE/SAU/UI0009/2011); SFRH/BD/87836/2012 to M.C. and SFRH/BPD/88322/2012 to L.G.]; and by Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO [(UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728)].BACKGROUND: Gold-nanobeacons (Au-nanobeacons) have proven to be versatile systems for molecular diagnostics and therapeutic actuators. Here, we present the development and characterization of two gold nanobeacons combined with Förster resonance energy transfer (FRET) based spectral codification for dual mode sequence discrimination. This is the combination of two powerful technologies onto a single nanosystem. RESULTS: We proved this concept to detect the most common fusion sequences associated with the development of chronic myeloid leukemia, e13a2 and e14a2. The detection is based on spectral shift of the donor signal to the acceptor, which allows for corroboration of the hybridization event. The Au-nanobeacon acts as scaffold for detection of the target in a homogenous format whose output capability (i.e. additional layer of information) is potentiated via the spectral codification strategy. CONCLUSIONS: The spectral coded Au-nanobeacons permit the detection of each of the pathogenic fusion sequences, with high specificity towards partial complementary sequences. The proposed BioCode Au-nanobeacon concept provides for a nanoplatform for molecular recognition suitable for cancer diagnostics.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaDCV - Departamento de Ciências da VidaLAQV@REQUIMTERUNCordeiro, M.Giestas, L.Lima, J. C.Baptista, P. M. V.2018-07-11T22:14:43Z2016-05-172016-05-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/41422eng1477-3155PURE: 4044669https://doi.org/10.1186/s12951-016-0192-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:22:20Zoai:run.unl.pt:10362/41422Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:31:21.486207Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia
title BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia
spellingShingle BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia
Cordeiro, M.
Biosensing Techniques
Fluorescence Resonance Energy Transfer
Fusion Proteins, bcr-abl
Gene Expression Regulation, Leukemic
Gold
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Metal Nanoparticles
Nucleic Acid Hybridization
Journal Article
SDG 3 - Good Health and Well-being
title_short BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia
title_full BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia
title_fullStr BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia
title_full_unstemmed BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia
title_sort BioCode gold-nanobeacon for the detection of fusion transcripts causing chronic myeloid leukemia
author Cordeiro, M.
author_facet Cordeiro, M.
Giestas, L.
Lima, J. C.
Baptista, P. M. V.
author_role author
author2 Giestas, L.
Lima, J. C.
Baptista, P. M. V.
author2_role author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
DCV - Departamento de Ciências da Vida
LAQV@REQUIMTE
RUN
dc.contributor.author.fl_str_mv Cordeiro, M.
Giestas, L.
Lima, J. C.
Baptista, P. M. V.
dc.subject.por.fl_str_mv Biosensing Techniques
Fluorescence Resonance Energy Transfer
Fusion Proteins, bcr-abl
Gene Expression Regulation, Leukemic
Gold
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Metal Nanoparticles
Nucleic Acid Hybridization
Journal Article
SDG 3 - Good Health and Well-being
topic Biosensing Techniques
Fluorescence Resonance Energy Transfer
Fusion Proteins, bcr-abl
Gene Expression Regulation, Leukemic
Gold
Humans
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Metal Nanoparticles
Nucleic Acid Hybridization
Journal Article
SDG 3 - Good Health and Well-being
description This work was supported by Fundacao para a Ciencia e Tecnologia, Ministry of Science and Education (FCT/MEC) [PTDC/QUI-QUI/112597/2009; PTDC/BBB-NAN/1812/2012; CIGMH (PEST-OE/SAU/UI0009/2011); SFRH/BD/87836/2012 to M.C. and SFRH/BPD/88322/2012 to L.G.]; and by Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO [(UID/Multi/04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728)].
publishDate 2016
dc.date.none.fl_str_mv 2016-05-17
2016-05-17T00:00:00Z
2018-07-11T22:14:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/41422
url http://hdl.handle.net/10362/41422
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1477-3155
PURE: 4044669
https://doi.org/10.1186/s12951-016-0192-y
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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