Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/5110 |
Resumo: | The aim of the present work is to provide insight into the mechanism of laccase reactions using syringyl-type mediators. We studied the pH dependence and the kinetics of oxidation of syringyl-type phenolics using the low CotA and the high redox potential TvL laccases. Additionally, the efficiency of these compounds as redox mediators for the oxidation of non-phenolic lignin units was tested at different pH values and increasing mediator/non-phenolic ratios. Finally, the intermediates and products of reactions were identified by LC-MS and H-1 NMR. These approaches allow concluding on the (1) mechanism involved in the oxidation of phenolics by bacterial laccases, (2) importance of the chemical nature and properties of phenolic mediators, (3) apparent independence of the enzyme's properties on the yields of non-phenolics conversion, (4) competitive routes involved in the catalytic cycle of the laccase-mediator system with several new C-O coupling type structures being proposed. |
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Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccasesLaccasesPhenolicsNatural MediatorsNon-PhenolicLignin UnitsRadical CouplingNatural MediatorsCrystal-StructureTrametes-VersicolorEndospore CoatPaper PulpOxidationLigninComponentSystemsFlaxThe aim of the present work is to provide insight into the mechanism of laccase reactions using syringyl-type mediators. We studied the pH dependence and the kinetics of oxidation of syringyl-type phenolics using the low CotA and the high redox potential TvL laccases. Additionally, the efficiency of these compounds as redox mediators for the oxidation of non-phenolic lignin units was tested at different pH values and increasing mediator/non-phenolic ratios. Finally, the intermediates and products of reactions were identified by LC-MS and H-1 NMR. These approaches allow concluding on the (1) mechanism involved in the oxidation of phenolics by bacterial laccases, (2) importance of the chemical nature and properties of phenolic mediators, (3) apparent independence of the enzyme's properties on the yields of non-phenolics conversion, (4) competitive routes involved in the catalytic cycle of the laccase-mediator system with several new C-O coupling type structures being proposed.Elsevier Sci LTDRCIPLRosado, TâniaBernardo, PedroKoci, KamilaCoelho, Ana V.Robalo, Maria PaulaMartins, Lígia O.2015-09-08T14:50:13Z2012-112012-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.21/5110engROSADO, T.; [et al] – Methyl syringate: Na efficient phenolic mediator for bacterial and fungal laccases. Bioresource Technology. ISSN: 0960-8524. Vol. 124 (2012), pp. 371-3780960-852410.1016/j.biortech.2012.08.023metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T09:48:03Zoai:repositorio.ipl.pt:10400.21/5110Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:14:26.088997Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases |
title |
Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases |
spellingShingle |
Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases Rosado, Tânia Laccases Phenolics Natural Mediators Non-Phenolic Lignin Units Radical Coupling Natural Mediators Crystal-Structure Trametes-Versicolor Endospore Coat Paper Pulp Oxidation Lignin Component Systems Flax |
title_short |
Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases |
title_full |
Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases |
title_fullStr |
Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases |
title_full_unstemmed |
Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases |
title_sort |
Methyl syringate: An efficient phenolic mediator for bacterial and fungal laccases |
author |
Rosado, Tânia |
author_facet |
Rosado, Tânia Bernardo, Pedro Koci, Kamila Coelho, Ana V. Robalo, Maria Paula Martins, Lígia O. |
author_role |
author |
author2 |
Bernardo, Pedro Koci, Kamila Coelho, Ana V. Robalo, Maria Paula Martins, Lígia O. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Rosado, Tânia Bernardo, Pedro Koci, Kamila Coelho, Ana V. Robalo, Maria Paula Martins, Lígia O. |
dc.subject.por.fl_str_mv |
Laccases Phenolics Natural Mediators Non-Phenolic Lignin Units Radical Coupling Natural Mediators Crystal-Structure Trametes-Versicolor Endospore Coat Paper Pulp Oxidation Lignin Component Systems Flax |
topic |
Laccases Phenolics Natural Mediators Non-Phenolic Lignin Units Radical Coupling Natural Mediators Crystal-Structure Trametes-Versicolor Endospore Coat Paper Pulp Oxidation Lignin Component Systems Flax |
description |
The aim of the present work is to provide insight into the mechanism of laccase reactions using syringyl-type mediators. We studied the pH dependence and the kinetics of oxidation of syringyl-type phenolics using the low CotA and the high redox potential TvL laccases. Additionally, the efficiency of these compounds as redox mediators for the oxidation of non-phenolic lignin units was tested at different pH values and increasing mediator/non-phenolic ratios. Finally, the intermediates and products of reactions were identified by LC-MS and H-1 NMR. These approaches allow concluding on the (1) mechanism involved in the oxidation of phenolics by bacterial laccases, (2) importance of the chemical nature and properties of phenolic mediators, (3) apparent independence of the enzyme's properties on the yields of non-phenolics conversion, (4) competitive routes involved in the catalytic cycle of the laccase-mediator system with several new C-O coupling type structures being proposed. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11 2012-11-01T00:00:00Z 2015-09-08T14:50:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/5110 |
url |
http://hdl.handle.net/10400.21/5110 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
ROSADO, T.; [et al] – Methyl syringate: Na efficient phenolic mediator for bacterial and fungal laccases. Bioresource Technology. ISSN: 0960-8524. Vol. 124 (2012), pp. 371-378 0960-8524 10.1016/j.biortech.2012.08.023 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Sci LTD |
publisher.none.fl_str_mv |
Elsevier Sci LTD |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133402401603584 |