Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus

Detalhes bibliográficos
Autor(a) principal: Pjeta, Robert
Data de Publicação: 2020
Outros Autores: Lindner, Herbert, Kremser, Leopold, Salvenmoser, Willi, Sobral, Daniel, Ladurner, Peter, Santos, Romana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/119325
Resumo: IF/00006/2015/CP1276/CT0001 P 25404-B25 P 30347
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spelling Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividusBioadhesionDifferential RNAseqEchinodermIn situ hybridizationMass spectrometrySea urchinCatalysisMolecular BiologySpectroscopyComputer Science ApplicationsPhysical and Theoretical ChemistryOrganic ChemistryInorganic ChemistryIF/00006/2015/CP1276/CT0001 P 25404-B25 P 30347Echinoderms, such as the rock-boring sea urchin Paracentrotus lividus, attach temporarily to surfaces during locomotion using their tube feet. They can attach firmly to any substrate and release from it within seconds through the secretion of unknown molecules. The composition of the adhesive, as well as the releasing secretion, remains largely unknown. This study re-analyzed a differential proteome dataset from Lebesgue et al. by mapping mass spectrometry-derived peptides to a P. lividus de novo transcriptome generated in this study. This resulted in a drastic increase in mapped proteins in comparison to the previous publication. The data were subsequently combined with a differential RNAseq approach to identify potential adhesion candidate genes. A gene expression analysis of 59 transcripts using whole mount in situ hybridization led to the identification of 16 transcripts potentially involved in bioadhesion. In the future these data could be useful for the production of synthetic reversible adhesives for industrial and medical purposes.DCV - Departamento de Ciências da VidaRUNPjeta, RobertLindner, HerbertKremser, LeopoldSalvenmoser, WilliSobral, DanielLadurner, PeterSantos, Romana2021-06-15T22:21:29Z2020-022020-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/119325eng1661-6596PURE: 31970348https://doi.org/10.3390/ijms21030946info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:01:51Zoai:run.unl.pt:10362/119325Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:44:02.055112Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus
title Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus
spellingShingle Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus
Pjeta, Robert
Bioadhesion
Differential RNAseq
Echinoderm
In situ hybridization
Mass spectrometry
Sea urchin
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
title_short Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus
title_full Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus
title_fullStr Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus
title_full_unstemmed Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus
title_sort Integrative transcriptome and proteome analysis of the tube foot and adhesive secretions of the sea urchin paracentrotus lividus
author Pjeta, Robert
author_facet Pjeta, Robert
Lindner, Herbert
Kremser, Leopold
Salvenmoser, Willi
Sobral, Daniel
Ladurner, Peter
Santos, Romana
author_role author
author2 Lindner, Herbert
Kremser, Leopold
Salvenmoser, Willi
Sobral, Daniel
Ladurner, Peter
Santos, Romana
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Pjeta, Robert
Lindner, Herbert
Kremser, Leopold
Salvenmoser, Willi
Sobral, Daniel
Ladurner, Peter
Santos, Romana
dc.subject.por.fl_str_mv Bioadhesion
Differential RNAseq
Echinoderm
In situ hybridization
Mass spectrometry
Sea urchin
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
topic Bioadhesion
Differential RNAseq
Echinoderm
In situ hybridization
Mass spectrometry
Sea urchin
Catalysis
Molecular Biology
Spectroscopy
Computer Science Applications
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
description IF/00006/2015/CP1276/CT0001 P 25404-B25 P 30347
publishDate 2020
dc.date.none.fl_str_mv 2020-02
2020-02-01T00:00:00Z
2021-06-15T22:21:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/119325
url http://hdl.handle.net/10362/119325
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1661-6596
PURE: 31970348
https://doi.org/10.3390/ijms21030946
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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