A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis

Detalhes bibliográficos
Autor(a) principal: Sá-Nogueira, Isabel de
Data de Publicação: 2010
Outros Autores: Ferreira, Mário José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/4199
Resumo: Journal of Bacteriology (Out 2010) 5312-5318
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spelling A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilisJournal of Bacteriology (Out 2010) 5312-5318Bacillus subtilis is able to utilize arabinopolysaccharides derived from plant biomass. Here, by combining genetic and physiological analyses we characterize the AraNPQ importer and identify primary and secondary transporters of B. subtilis involved in the uptake of arabinosaccharides. We show that the ABC-type importer AraNPQ is involved in the uptake of α-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units. Although this system is the key transporter for α-1,5-arabinotriose and α-1,5-arabinotetraose, the results indicate that α-1,5-arabinobiose also is translocated by the secondary transporter AraE. This broad-specificity proton symporter is the major transporter for arabinose and also is accountable for the uptake of xylose and galactose. In addition, MsmX is shown to be the ATPase that energizes the incomplete AraNPQ importer. Furthermore, the results suggest the existence of at least one more unidentified MsmX-dependent ABC importer responsible for the uptake of nonlinear α-1,2- and α-1,3-arabinooligosaccharides. This study assigns MsmX as a multipurpose B. subtilis ATPase required to energize different saccharide transporters, the arabinooligosaccharide-specific AraNPQ-MsmX system, a putative MsmX-dependent ABC transporter specific for nonlinear arabinooligosaccharides, and the previously characterized maltodextrin-specific MdxEFG-MsmX system.This work was supported partially by grant no. PTDC/AGR-AAM/102345/2008 from Fundacao para a Ciencia e Tecnologia (FCT), FEDER, and POFC 2010, to I.S.-N.American Society for MicrobiologyRUNSá-Nogueira, Isabel deFerreira, Mário José2010-10-22T14:43:37Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/4199engFerreira M.J. and I. de Sá-Nogueira (2010) A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis. Journal of Bacteriology 192 (20): 5312-53180021-9193 (Print)info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:33:52Zoai:run.unl.pt:10362/4199Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:15:35.533442Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis
title A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis
spellingShingle A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis
Sá-Nogueira, Isabel de
title_short A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis
title_full A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis
title_fullStr A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis
title_full_unstemmed A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis
title_sort A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis
author Sá-Nogueira, Isabel de
author_facet Sá-Nogueira, Isabel de
Ferreira, Mário José
author_role author
author2 Ferreira, Mário José
author2_role author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Sá-Nogueira, Isabel de
Ferreira, Mário José
description Journal of Bacteriology (Out 2010) 5312-5318
publishDate 2010
dc.date.none.fl_str_mv 2010-10-22T14:43:37Z
2010
2010-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/4199
url http://hdl.handle.net/10362/4199
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ferreira M.J. and I. de Sá-Nogueira (2010) A multitask ATPase serving different ABC-type sugar importers in Bacillus subtilis. Journal of Bacteriology 192 (20): 5312-5318
0021-9193 (Print)
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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