Microbiological contamination in Portuguese firefighters headquarters

Detalhes bibliográficos
Autor(a) principal: Gomes, Bianca Rosário
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/56099
Resumo: Tese de mestrado, Biologia Humana e Ambiente, 2021, Universidade de Lisboa, Faculdade de Ciências
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spelling Microbiological contamination in Portuguese firefighters headquartersExposição OcupacionalContaminação microbiológicaAspergillus fumigatusPerfil de resistência fúngicaTeses de mestrado - 2021Departamento de Biologia AnimalTese de mestrado, Biologia Humana e Ambiente, 2021, Universidade de Lisboa, Faculdade de CiênciasIn occupational environments, employees might be exposed to a diversity of microbiological agents. However, less is known about firefighter’s exposure in the work environment. Thus, this study aims to characterize the microbiological contamination in firefighter’s headquarters from Lisbon, Portugal. SARS-CoV-2 and fungal resistance profile assessment were also performed. Eleven firefighters headquarters (FFH) were assessed and the sampling campaign comprised active and passive sampling methods. The microbiological assessment covered culture-dependent and culture-independent methods. Additionally, to perform the azole resistance screening the samples extracts were inoculated in Sabouraud dextrose agar (SDA) azole supplemented media (Itraconazole (ITR), Voriconazole (VOR) and Posaconazole (POS)). In general, from all the matrices the predominant genera were Cladosporium and Penicillium. Regarding microbiological load indoors, in accordance with the scientific criteria for occupational exposure assessment (I /O ≤1), from the 11 FFH sampled, 5 (45.45%) surpassed the stipulated value in what concerns fungal levels. The same trend was reported in 7 FFH (63.63%) for bacteria. Moreover, when using the World Health Organization (WHO) suggested indoor guideline (maximum value of 150 CFU.m-3 ), the same FFH were above the stipulated limits for fungal and bacterial load respectively. Overall, the use of a multi-approach protocol for sampling and analyses allowed a broader microbial characterization. Also, fungal growth in azole supplemented media suggests the presence of multidrug resistance. Thus, further antifungal susceptibility tests should be preconized. The molecular detection of sections Fumigati and Nidulantes in additional matrices suggests molecular tools as a suitable approach overcoming culture-dependent methods limitations. Regarding SARS-CoV-2 assessment, no detection was obtained which suggest the effectiveness of cleaning in viral control. In short, these facilities are an occupational environment to have into consideration regarding the microbiological contamination. In fact, more research is needed in order to implement proper measures to reduce workers risk and minimize the exposure.Carla, ViegasRebelo, Maria Teresa Ferreira Ramos Nabais de Oliveira, 1964-Repositório da Universidade de LisboaGomes, Bianca Rosário2023-02-01T12:49:04Z202120212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/56099TID:202934896enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:03:29Zoai:repositorio.ul.pt:10451/56099Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:06:39.201183Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microbiological contamination in Portuguese firefighters headquarters
title Microbiological contamination in Portuguese firefighters headquarters
spellingShingle Microbiological contamination in Portuguese firefighters headquarters
Gomes, Bianca Rosário
Exposição Ocupacional
Contaminação microbiológica
Aspergillus fumigatus
Perfil de resistência fúngica
Teses de mestrado - 2021
Departamento de Biologia Animal
title_short Microbiological contamination in Portuguese firefighters headquarters
title_full Microbiological contamination in Portuguese firefighters headquarters
title_fullStr Microbiological contamination in Portuguese firefighters headquarters
title_full_unstemmed Microbiological contamination in Portuguese firefighters headquarters
title_sort Microbiological contamination in Portuguese firefighters headquarters
author Gomes, Bianca Rosário
author_facet Gomes, Bianca Rosário
author_role author
dc.contributor.none.fl_str_mv Carla, Viegas
Rebelo, Maria Teresa Ferreira Ramos Nabais de Oliveira, 1964-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gomes, Bianca Rosário
dc.subject.por.fl_str_mv Exposição Ocupacional
Contaminação microbiológica
Aspergillus fumigatus
Perfil de resistência fúngica
Teses de mestrado - 2021
Departamento de Biologia Animal
topic Exposição Ocupacional
Contaminação microbiológica
Aspergillus fumigatus
Perfil de resistência fúngica
Teses de mestrado - 2021
Departamento de Biologia Animal
description Tese de mestrado, Biologia Humana e Ambiente, 2021, Universidade de Lisboa, Faculdade de Ciências
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021-01-01T00:00:00Z
2023-02-01T12:49:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/56099
TID:202934896
url http://hdl.handle.net/10451/56099
identifier_str_mv TID:202934896
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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