Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contraction

Detalhes bibliográficos
Autor(a) principal: Calejo, Joana
Data de Publicação: 2016
Outros Autores: Pinho, Diana, Galindo-Rosales, Francisco J., Lima, Rui Alberto Madeira Macedo, Campo-Deaño, Laura
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/51180
Resumo: The interest in the development of blood analogues has been increasing recently as a consequence of the increment in the number of experimental hemodynamic studies and the difficulties associated with the manipulation of real blood in vitro because of ethical, economical or hazardous issues. Although one-phase Newtonian and non-Newtonian blood analogues can be found in the literature, there are very few studies related to the use of particulate solutions in which the particles mimic the behaviour of the red blood cells (RBCs) or erythrocytes. One of the most relevant effects related with the behaviour of the erythrocytes is a cell-free layer (CFL) formation, which consists in the migration of the RBCs towards the center of the vessel forming a cell depleted plasma region near the vessel walls, which is known to happen in in vitro microcirculatory environments. Recent studies have shown that the CFL enhancement is possible with an insertion of contraction and expansion region in a straight microchannel. These effects are useful for cell manipulation or sorting in lab-on-chip studies. In this experimental study we present particulate Newtonian and non-Newtonian solutions which resulted in a rheological blood analogue able to form a CFL, downstream of a microfluidic hyperbolic contraction, in a similar way of the one formed by healthy RBCs.
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spelling Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contractionHemodynamicsBlood analogueRheologyMicrofluidicsCell-free layerScience & TechnologyThe interest in the development of blood analogues has been increasing recently as a consequence of the increment in the number of experimental hemodynamic studies and the difficulties associated with the manipulation of real blood in vitro because of ethical, economical or hazardous issues. Although one-phase Newtonian and non-Newtonian blood analogues can be found in the literature, there are very few studies related to the use of particulate solutions in which the particles mimic the behaviour of the red blood cells (RBCs) or erythrocytes. One of the most relevant effects related with the behaviour of the erythrocytes is a cell-free layer (CFL) formation, which consists in the migration of the RBCs towards the center of the vessel forming a cell depleted plasma region near the vessel walls, which is known to happen in in vitro microcirculatory environments. Recent studies have shown that the CFL enhancement is possible with an insertion of contraction and expansion region in a straight microchannel. These effects are useful for cell manipulation or sorting in lab-on-chip studies. In this experimental study we present particulate Newtonian and non-Newtonian solutions which resulted in a rheological blood analogue able to form a CFL, downstream of a microfluidic hyperbolic contraction, in a similar way of the one formed by healthy RBCs.The authors acknowledge the financial support provided by Fundação para a Ciência e a Tecnologia (FCT), COMPETE and FEDER through projects PTDC/SAU-BEB/105650/2008, PTDC/SAU-ENB/116929/2010, PTDC/EQU-FTT/118716/2010, EXPL/EMS-SIS/2215/2013, EXPL/EMS-TRA/2306/2013, fellowships SFRH/BD/89077/2012, SFRH/BPD/69663/2010 and SFRH/BPD/69664/2010 and grants IF/00148/2013 and IF/00190/2013.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoCalejo, JoanaPinho, DianaGalindo-Rosales, Francisco J.Lima, Rui Alberto Madeira MacedoCampo-Deaño, Laura20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51180eng2072-666X10.3390/mi7010004info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:46:32Zoai:repositorium.sdum.uminho.pt:1822/51180Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:44:32.609490Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contraction
title Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contraction
spellingShingle Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contraction
Calejo, Joana
Hemodynamics
Blood analogue
Rheology
Microfluidics
Cell-free layer
Science & Technology
title_short Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contraction
title_full Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contraction
title_fullStr Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contraction
title_full_unstemmed Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contraction
title_sort Particulate blood analogues reproducing the erythrocytes cell-free layer in a microfluidic device containing a hyperbolic contraction
author Calejo, Joana
author_facet Calejo, Joana
Pinho, Diana
Galindo-Rosales, Francisco J.
Lima, Rui Alberto Madeira Macedo
Campo-Deaño, Laura
author_role author
author2 Pinho, Diana
Galindo-Rosales, Francisco J.
Lima, Rui Alberto Madeira Macedo
Campo-Deaño, Laura
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Calejo, Joana
Pinho, Diana
Galindo-Rosales, Francisco J.
Lima, Rui Alberto Madeira Macedo
Campo-Deaño, Laura
dc.subject.por.fl_str_mv Hemodynamics
Blood analogue
Rheology
Microfluidics
Cell-free layer
Science & Technology
topic Hemodynamics
Blood analogue
Rheology
Microfluidics
Cell-free layer
Science & Technology
description The interest in the development of blood analogues has been increasing recently as a consequence of the increment in the number of experimental hemodynamic studies and the difficulties associated with the manipulation of real blood in vitro because of ethical, economical or hazardous issues. Although one-phase Newtonian and non-Newtonian blood analogues can be found in the literature, there are very few studies related to the use of particulate solutions in which the particles mimic the behaviour of the red blood cells (RBCs) or erythrocytes. One of the most relevant effects related with the behaviour of the erythrocytes is a cell-free layer (CFL) formation, which consists in the migration of the RBCs towards the center of the vessel forming a cell depleted plasma region near the vessel walls, which is known to happen in in vitro microcirculatory environments. Recent studies have shown that the CFL enhancement is possible with an insertion of contraction and expansion region in a straight microchannel. These effects are useful for cell manipulation or sorting in lab-on-chip studies. In this experimental study we present particulate Newtonian and non-Newtonian solutions which resulted in a rheological blood analogue able to form a CFL, downstream of a microfluidic hyperbolic contraction, in a similar way of the one formed by healthy RBCs.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/51180
url http://hdl.handle.net/1822/51180
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2072-666X
10.3390/mi7010004
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dc.publisher.none.fl_str_mv MDPI
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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