Antioxidant and Antimicrobial Activity of Plants from Portuguese Flora

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Alexandre
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/17539
Resumo: Through the years, we have seen the important role of plants in heallth, with valuable drugs being approved derived from phytochemicals. The diversity found in the plant kingdom makes that the properties of many medicinal plants remain unknown. Therefore, this research aims to evaluate the biological activity through antioxidante and antibacterial in vitro assays of three plants from Portuguese flora. It was prepared aqueous and methanolic extracts (AE amd ME, respectively) from Euphorbia paralias, Euphorbia hirsuta, and Trifolium tomentosum. Their total phenolic contente (TPC), capacity to scavenge DPPH radical, and ability to chelate iron ions were evaluated as well as their antibacterial activity agianst Escherichia coli and Staphylococcus aureus, through disc diffusion and broth microdilution methods. Euphorbia species presented their higher radical scavenging capacity (IC50˂50 µg/mL). The ME of E. paralias presented the highest TPC (293.37 ± 20.25 mg GAE/g) and also had the best performance in chelating iron ions. This same extract, along with ME of E. hirsuta, promoted moderate growth inhibition of S. aureus at 10 mg/ml with a MIC value of 1.0 and 2.0 mg/ml, respectively. Our findings provided preliminar knowledge about these plants and highlighted the potentialities of the Euphorbia genus.
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spelling Antioxidant and Antimicrobial Activity of Plants from Portuguese FloraEuphorbiaTrifoliumAntioxidantsAntibacterialPhytochemicalsThrough the years, we have seen the important role of plants in heallth, with valuable drugs being approved derived from phytochemicals. The diversity found in the plant kingdom makes that the properties of many medicinal plants remain unknown. Therefore, this research aims to evaluate the biological activity through antioxidante and antibacterial in vitro assays of three plants from Portuguese flora. It was prepared aqueous and methanolic extracts (AE amd ME, respectively) from Euphorbia paralias, Euphorbia hirsuta, and Trifolium tomentosum. Their total phenolic contente (TPC), capacity to scavenge DPPH radical, and ability to chelate iron ions were evaluated as well as their antibacterial activity agianst Escherichia coli and Staphylococcus aureus, through disc diffusion and broth microdilution methods. Euphorbia species presented their higher radical scavenging capacity (IC50˂50 µg/mL). The ME of E. paralias presented the highest TPC (293.37 ± 20.25 mg GAE/g) and also had the best performance in chelating iron ions. This same extract, along with ME of E. hirsuta, promoted moderate growth inhibition of S. aureus at 10 mg/ml with a MIC value of 1.0 and 2.0 mg/ml, respectively. Our findings provided preliminar knowledge about these plants and highlighted the potentialities of the Euphorbia genus.Pinho, Cláudia Marta Libreiro deOliveira, Ana Isabel de Freitas Tavares deRepositório Científico do Instituto Politécnico do PortoRibeiro, Alexandre2021-03-16T16:16:14Z2021-01-072021-01-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.22/17539TID:202637816enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T13:08:19Zoai:recipp.ipp.pt:10400.22/17539Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:37:09.654005Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antioxidant and Antimicrobial Activity of Plants from Portuguese Flora
title Antioxidant and Antimicrobial Activity of Plants from Portuguese Flora
spellingShingle Antioxidant and Antimicrobial Activity of Plants from Portuguese Flora
Ribeiro, Alexandre
Euphorbia
Trifolium
Antioxidants
Antibacterial
Phytochemicals
title_short Antioxidant and Antimicrobial Activity of Plants from Portuguese Flora
title_full Antioxidant and Antimicrobial Activity of Plants from Portuguese Flora
title_fullStr Antioxidant and Antimicrobial Activity of Plants from Portuguese Flora
title_full_unstemmed Antioxidant and Antimicrobial Activity of Plants from Portuguese Flora
title_sort Antioxidant and Antimicrobial Activity of Plants from Portuguese Flora
author Ribeiro, Alexandre
author_facet Ribeiro, Alexandre
author_role author
dc.contributor.none.fl_str_mv Pinho, Cláudia Marta Libreiro de
Oliveira, Ana Isabel de Freitas Tavares de
Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Ribeiro, Alexandre
dc.subject.por.fl_str_mv Euphorbia
Trifolium
Antioxidants
Antibacterial
Phytochemicals
topic Euphorbia
Trifolium
Antioxidants
Antibacterial
Phytochemicals
description Through the years, we have seen the important role of plants in heallth, with valuable drugs being approved derived from phytochemicals. The diversity found in the plant kingdom makes that the properties of many medicinal plants remain unknown. Therefore, this research aims to evaluate the biological activity through antioxidante and antibacterial in vitro assays of three plants from Portuguese flora. It was prepared aqueous and methanolic extracts (AE amd ME, respectively) from Euphorbia paralias, Euphorbia hirsuta, and Trifolium tomentosum. Their total phenolic contente (TPC), capacity to scavenge DPPH radical, and ability to chelate iron ions were evaluated as well as their antibacterial activity agianst Escherichia coli and Staphylococcus aureus, through disc diffusion and broth microdilution methods. Euphorbia species presented their higher radical scavenging capacity (IC50˂50 µg/mL). The ME of E. paralias presented the highest TPC (293.37 ± 20.25 mg GAE/g) and also had the best performance in chelating iron ions. This same extract, along with ME of E. hirsuta, promoted moderate growth inhibition of S. aureus at 10 mg/ml with a MIC value of 1.0 and 2.0 mg/ml, respectively. Our findings provided preliminar knowledge about these plants and highlighted the potentialities of the Euphorbia genus.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-16T16:16:14Z
2021-01-07
2021-01-07T00:00:00Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/17539
TID:202637816
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identifier_str_mv TID:202637816
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