Intraepithelial nerve fibers project into the lumen of the larynx
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/1689 |
Resumo: | Objectives/Hypothesis: Studies on the morphology and location of the sensory receptors in the laryngeal mucosa have resulted in insufficient and sometimes conflicting data. In the present study the authors analyzed the distribution and morphology of sensory nerve plexuses and terminal fibers in the laryngeal mucosa of the rat. Study Design: Two groups of Male Wistar rats were used in this laboratory study; the larynx of the first group were used to analyse the sensitive innervation of its epithelium, whereas the larynx of the second group (controls) were tested for the specificity of the antibodies used. Methods: The larynges of the animals were entirely removed after perfusion, and coronal or horizontal sections were immunoprocessed for further randomized analysis of the mucosa. Primary afferents were detected by immunoreaction to two widely recognized markers of sensory nerves, calcitonin generelated peptide and substance P, and visualized using diaminobenzidine as a chromogen. Results: The nerve plexuses were more densely distributed in the dorsal half of the vocal folds and in the laryngeal aspect of the epiglottis. Dense networks of fine fibers with many varicosities en passant, immunoreactive for both calcitonin gene-related peptide and substance P, occurred in the lamina propria and along the epithelial thickness. Calcitonin gene-related peptide– immunoreactive and substance P–immunoreactive fibers extended across the epithelium and projected to the laryngeal lumen itself, reaching the space between the cilia. Conclusion: The projection of intraepithelial nerve fibers into the lumen of the larynx indicates that in the absence of mucus, nerve endings may be exposed and thus receive direct stimulation from airborne substances. Furthermore, it suggests that the laryngeal mucosa of the rat may constitute an experimental model for studying the direct activation or manipulation of primary afferents at the periphery and neurogenic inflammation. |
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Intraepithelial nerve fibers project into the lumen of the larynxLarynxSensory innervationRatSubstance PCGRPlight microscopycalcitonin gene-related peptideprimary afferentslaryngeal mucosaintraepithelialScience & TechnologyObjectives/Hypothesis: Studies on the morphology and location of the sensory receptors in the laryngeal mucosa have resulted in insufficient and sometimes conflicting data. In the present study the authors analyzed the distribution and morphology of sensory nerve plexuses and terminal fibers in the laryngeal mucosa of the rat. Study Design: Two groups of Male Wistar rats were used in this laboratory study; the larynx of the first group were used to analyse the sensitive innervation of its epithelium, whereas the larynx of the second group (controls) were tested for the specificity of the antibodies used. Methods: The larynges of the animals were entirely removed after perfusion, and coronal or horizontal sections were immunoprocessed for further randomized analysis of the mucosa. Primary afferents were detected by immunoreaction to two widely recognized markers of sensory nerves, calcitonin generelated peptide and substance P, and visualized using diaminobenzidine as a chromogen. Results: The nerve plexuses were more densely distributed in the dorsal half of the vocal folds and in the laryngeal aspect of the epiglottis. Dense networks of fine fibers with many varicosities en passant, immunoreactive for both calcitonin gene-related peptide and substance P, occurred in the lamina propria and along the epithelial thickness. Calcitonin gene-related peptide– immunoreactive and substance P–immunoreactive fibers extended across the epithelium and projected to the laryngeal lumen itself, reaching the space between the cilia. Conclusion: The projection of intraepithelial nerve fibers into the lumen of the larynx indicates that in the absence of mucus, nerve endings may be exposed and thus receive direct stimulation from airborne substances. Furthermore, it suggests that the laryngeal mucosa of the rat may constitute an experimental model for studying the direct activation or manipulation of primary afferents at the periphery and neurogenic inflammation.União Europeia (UE). Fundo Europeu de Desenvolvimento Regional (FEDER).Fundação para a Ciência e a Tecnologia (FCT) – Programa Operacional “Ciência, Tecnologia, Inovação” (POCTI) - POCTI/NSE/46399/2002.Grunenthal Foundation.Lippincott, Williams & WilkinsUniversidade do MinhoRodrigues, Manuel LimaNunes, RuiAlmeida, Armando2004-062004-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1689eng"Laryngoscope". ISSN 0023-852X. 114:6 (2004) 1074–1077.0023-852X10.1097/00005537-200406000-0002215179216http://www.lwwonline.com/pt/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:05:38Zoai:repositorium.sdum.uminho.pt:1822/1689Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:56:06.976025Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Intraepithelial nerve fibers project into the lumen of the larynx |
title |
Intraepithelial nerve fibers project into the lumen of the larynx |
spellingShingle |
Intraepithelial nerve fibers project into the lumen of the larynx Rodrigues, Manuel Lima Larynx Sensory innervation Rat Substance P CGRP light microscopy calcitonin gene-related peptide primary afferents laryngeal mucosa intraepithelial Science & Technology |
title_short |
Intraepithelial nerve fibers project into the lumen of the larynx |
title_full |
Intraepithelial nerve fibers project into the lumen of the larynx |
title_fullStr |
Intraepithelial nerve fibers project into the lumen of the larynx |
title_full_unstemmed |
Intraepithelial nerve fibers project into the lumen of the larynx |
title_sort |
Intraepithelial nerve fibers project into the lumen of the larynx |
author |
Rodrigues, Manuel Lima |
author_facet |
Rodrigues, Manuel Lima Nunes, Rui Almeida, Armando |
author_role |
author |
author2 |
Nunes, Rui Almeida, Armando |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Rodrigues, Manuel Lima Nunes, Rui Almeida, Armando |
dc.subject.por.fl_str_mv |
Larynx Sensory innervation Rat Substance P CGRP light microscopy calcitonin gene-related peptide primary afferents laryngeal mucosa intraepithelial Science & Technology |
topic |
Larynx Sensory innervation Rat Substance P CGRP light microscopy calcitonin gene-related peptide primary afferents laryngeal mucosa intraepithelial Science & Technology |
description |
Objectives/Hypothesis: Studies on the morphology and location of the sensory receptors in the laryngeal mucosa have resulted in insufficient and sometimes conflicting data. In the present study the authors analyzed the distribution and morphology of sensory nerve plexuses and terminal fibers in the laryngeal mucosa of the rat. Study Design: Two groups of Male Wistar rats were used in this laboratory study; the larynx of the first group were used to analyse the sensitive innervation of its epithelium, whereas the larynx of the second group (controls) were tested for the specificity of the antibodies used. Methods: The larynges of the animals were entirely removed after perfusion, and coronal or horizontal sections were immunoprocessed for further randomized analysis of the mucosa. Primary afferents were detected by immunoreaction to two widely recognized markers of sensory nerves, calcitonin generelated peptide and substance P, and visualized using diaminobenzidine as a chromogen. Results: The nerve plexuses were more densely distributed in the dorsal half of the vocal folds and in the laryngeal aspect of the epiglottis. Dense networks of fine fibers with many varicosities en passant, immunoreactive for both calcitonin gene-related peptide and substance P, occurred in the lamina propria and along the epithelial thickness. Calcitonin gene-related peptide– immunoreactive and substance P–immunoreactive fibers extended across the epithelium and projected to the laryngeal lumen itself, reaching the space between the cilia. Conclusion: The projection of intraepithelial nerve fibers into the lumen of the larynx indicates that in the absence of mucus, nerve endings may be exposed and thus receive direct stimulation from airborne substances. Furthermore, it suggests that the laryngeal mucosa of the rat may constitute an experimental model for studying the direct activation or manipulation of primary afferents at the periphery and neurogenic inflammation. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06 2004-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/1689 |
url |
http://hdl.handle.net/1822/1689 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Laryngoscope". ISSN 0023-852X. 114:6 (2004) 1074–1077. 0023-852X 10.1097/00005537-200406000-00022 15179216 http://www.lwwonline.com/pt/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Lippincott, Williams & Wilkins |
publisher.none.fl_str_mv |
Lippincott, Williams & Wilkins |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132348208381952 |