Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model

Detalhes bibliográficos
Autor(a) principal: Gregório, Ana C.
Data de Publicação: 2016
Outros Autores: Fonseca, Nuno A., Moura, Vera, Lacerda, Manuela, Figueiredo, Paulo, Simões, Sérgio, Dias, Sérgio, Moreira, João Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/48721
Resumo: Copyright: © 2016 Gregório et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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spelling Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine modelCopyright: © 2016 Gregório et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.4T1 metastatic breast cancer model have been widely used to study stage IV human breast cancer. However, the frequent inoculation of a large number of cells, gives rise to fast growing tumors, as well as to a surprisingly low metastatic take rate. The present work aimed at establishing the conditions enabling high metastatic take rate of the triple-negative murine 4T1 syngeneic breast cancer model. An 87% 4T1 tumor incidence was observed when as few as 500 cancer cells were implanted. 4T1 cancer cells colonized primarily the lungs with 100% efficiency, and distant lesions were also commonly identified in the mesentery and pancreas. The drastic reduction of the number of inoculated cells resulted in increased tumor doubling times and decreased specific growth rates, following a Gompertzian tumor expansion. The established conditions for the 4T1 mouse model were further validated in a therapeutic study with peguilated liposomal doxorubicin, in clinical used in the setting of metastatic breast cancer. Inoculated cell density was proven to be a key methodological aspect towards the reproducible development of macrometastases in the 4T1 mouse model and a more reliable pre-clinical assessment of antimetastatic therapies.Ana Cristina Gregório is a student of the international PhD program in Experimental Biology and Biomedicine (PDBEB) from the Institute for Interdisciplinary Research, University of Coimbra and recipient of the fellowship SFRH/BD/51190/2010 from the Portuguese Foundation for Science and Technology (FCT). The work was supported by the grants PTDC/SAU-BMA/121028/2010 (FCT) and UID/NEU/04539/2013 (FEDER/COMPETE 2020/FCT).PLOSRepositório da Universidade de LisboaGregório, Ana C.Fonseca, Nuno A.Moura, VeraLacerda, ManuelaFigueiredo, PauloSimões, SérgioDias, SérgioMoreira, João Nuno2021-06-23T13:30:14Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/48721engPLoS One. 2016 Nov 7;11(11):e0165817.10.1371/journal.pone.01658171932-6203info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:52:06Zoai:repositorio.ul.pt:10451/48721Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:28.960786Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model
title Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model
spellingShingle Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model
Gregório, Ana C.
title_short Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model
title_full Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model
title_fullStr Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model
title_full_unstemmed Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model
title_sort Inoculated cell density as a determinant factor of the growth dynamics and metastatic efficiency of a breast cancer murine model
author Gregório, Ana C.
author_facet Gregório, Ana C.
Fonseca, Nuno A.
Moura, Vera
Lacerda, Manuela
Figueiredo, Paulo
Simões, Sérgio
Dias, Sérgio
Moreira, João Nuno
author_role author
author2 Fonseca, Nuno A.
Moura, Vera
Lacerda, Manuela
Figueiredo, Paulo
Simões, Sérgio
Dias, Sérgio
Moreira, João Nuno
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gregório, Ana C.
Fonseca, Nuno A.
Moura, Vera
Lacerda, Manuela
Figueiredo, Paulo
Simões, Sérgio
Dias, Sérgio
Moreira, João Nuno
description Copyright: © 2016 Gregório et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
2021-06-23T13:30:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/48721
url http://hdl.handle.net/10451/48721
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PLoS One. 2016 Nov 7;11(11):e0165817.
10.1371/journal.pone.0165817
1932-6203
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv PLOS
publisher.none.fl_str_mv PLOS
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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