Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes

Detalhes bibliográficos
Autor(a) principal: Martins, Ana M.
Data de Publicação: 2010
Outros Autores: Saraf, A., Sousa, R. A., Alves, C. M., Mikos, Antonios G., Kasper, F. Kurtis, Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/14041
Resumo: Previous studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteogenic differentiation of MSCs. SPCL fiber meshes were seeded with MSCs and cultured with osteogenic medium supplemented with a-amylase, lipase, or a combination of the two for 8 or 16 days using static or flow conditions. Lipase and its combination with a-amylase enhanced cell proliferation after 16 days. In addition, the flow perfusion culture enhanced the infiltration of cells and facilitated greater distribution of extracellular matrix (ECM) throughout the scaffolds in the presence/absence of enzymes. A significant amount of calcium was detected after 16 days in all groups cultured in flow conditions compared with static cultures. Nevertheless, when a-amylase and lipase were included in the flow perfusion cultures, the calcium content was 379 6 30 lg/scaffold after as few as 8 days. The highest calcium content (1271 6 32 lg/scaffold) was obtained for SPCL/cell constructs cultured for 16 days in the presence of lipase and flow. Furthermore, von Kossa staining and tetracycline fluorescence of histological sections demonstrated mineral deposition within the scaffolds for all groups cultured for 16 days under flow. However, all the data corroborate that lipase coupled with flow perfusion conditions improve the osteogenic differentiation of MSCs and enhance ECM mineralization.
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spelling Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshesStarch/poly(e-polycaprolactone) fiber meshesa-amylaseLipaseFlow perfusion bioreactorOsteogenicstarch/poly(epsilon-polycaprolactone) fiber meshesosteogenic differentiationStarch/poly(ε-polycaprolactone) fiber meshesScience & TechnologyPrevious studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteogenic differentiation of MSCs. SPCL fiber meshes were seeded with MSCs and cultured with osteogenic medium supplemented with a-amylase, lipase, or a combination of the two for 8 or 16 days using static or flow conditions. Lipase and its combination with a-amylase enhanced cell proliferation after 16 days. In addition, the flow perfusion culture enhanced the infiltration of cells and facilitated greater distribution of extracellular matrix (ECM) throughout the scaffolds in the presence/absence of enzymes. A significant amount of calcium was detected after 16 days in all groups cultured in flow conditions compared with static cultures. Nevertheless, when a-amylase and lipase were included in the flow perfusion cultures, the calcium content was 379 6 30 lg/scaffold after as few as 8 days. The highest calcium content (1271 6 32 lg/scaffold) was obtained for SPCL/cell constructs cultured for 16 days in the presence of lipase and flow. Furthermore, von Kossa staining and tetracycline fluorescence of histological sections demonstrated mineral deposition within the scaffolds for all groups cultured for 16 days under flow. However, all the data corroborate that lipase coupled with flow perfusion conditions improve the osteogenic differentiation of MSCs and enhance ECM mineralization.Contract grant sponsor: Portuguese Foundation for Science and Technology (FCT); contract grant number: SFRH/BPD/26763/2006Contract grant sponsor: European NoE EXPERTISSUES; contract grant number: NMP3-CT-2004-500283WileyUniversidade do MinhoMartins, Ana M.Saraf, A.Sousa, R. A.Alves, C. M.Mikos, Antonios G.Kasper, F. KurtisReis, R. L.2010-092010-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/14041eng1552-496510.1002/jbm.a.3278520694973info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:59Zoai:repositorium.sdum.uminho.pt:1822/14041Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:37:52.273709Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
title Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
spellingShingle Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
Martins, Ana M.
Starch/poly(e-polycaprolactone) fiber meshes
a-amylase
Lipase
Flow perfusion bioreactor
Osteogenic
starch/poly(epsilon-polycaprolactone) fiber meshes
osteogenic differentiation
Starch/poly(ε-polycaprolactone) fiber meshes
Science & Technology
title_short Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
title_full Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
title_fullStr Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
title_full_unstemmed Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
title_sort Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
author Martins, Ana M.
author_facet Martins, Ana M.
Saraf, A.
Sousa, R. A.
Alves, C. M.
Mikos, Antonios G.
Kasper, F. Kurtis
Reis, R. L.
author_role author
author2 Saraf, A.
Sousa, R. A.
Alves, C. M.
Mikos, Antonios G.
Kasper, F. Kurtis
Reis, R. L.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Martins, Ana M.
Saraf, A.
Sousa, R. A.
Alves, C. M.
Mikos, Antonios G.
Kasper, F. Kurtis
Reis, R. L.
dc.subject.por.fl_str_mv Starch/poly(e-polycaprolactone) fiber meshes
a-amylase
Lipase
Flow perfusion bioreactor
Osteogenic
starch/poly(epsilon-polycaprolactone) fiber meshes
osteogenic differentiation
Starch/poly(ε-polycaprolactone) fiber meshes
Science & Technology
topic Starch/poly(e-polycaprolactone) fiber meshes
a-amylase
Lipase
Flow perfusion bioreactor
Osteogenic
starch/poly(epsilon-polycaprolactone) fiber meshes
osteogenic differentiation
Starch/poly(ε-polycaprolactone) fiber meshes
Science & Technology
description Previous studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteogenic differentiation of MSCs. SPCL fiber meshes were seeded with MSCs and cultured with osteogenic medium supplemented with a-amylase, lipase, or a combination of the two for 8 or 16 days using static or flow conditions. Lipase and its combination with a-amylase enhanced cell proliferation after 16 days. In addition, the flow perfusion culture enhanced the infiltration of cells and facilitated greater distribution of extracellular matrix (ECM) throughout the scaffolds in the presence/absence of enzymes. A significant amount of calcium was detected after 16 days in all groups cultured in flow conditions compared with static cultures. Nevertheless, when a-amylase and lipase were included in the flow perfusion cultures, the calcium content was 379 6 30 lg/scaffold after as few as 8 days. The highest calcium content (1271 6 32 lg/scaffold) was obtained for SPCL/cell constructs cultured for 16 days in the presence of lipase and flow. Furthermore, von Kossa staining and tetracycline fluorescence of histological sections demonstrated mineral deposition within the scaffolds for all groups cultured for 16 days under flow. However, all the data corroborate that lipase coupled with flow perfusion conditions improve the osteogenic differentiation of MSCs and enhance ECM mineralization.
publishDate 2010
dc.date.none.fl_str_mv 2010-09
2010-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/14041
url https://hdl.handle.net/1822/14041
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1552-4965
10.1002/jbm.a.32785
20694973
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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