Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/14041 |
Resumo: | Previous studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteogenic differentiation of MSCs. SPCL fiber meshes were seeded with MSCs and cultured with osteogenic medium supplemented with a-amylase, lipase, or a combination of the two for 8 or 16 days using static or flow conditions. Lipase and its combination with a-amylase enhanced cell proliferation after 16 days. In addition, the flow perfusion culture enhanced the infiltration of cells and facilitated greater distribution of extracellular matrix (ECM) throughout the scaffolds in the presence/absence of enzymes. A significant amount of calcium was detected after 16 days in all groups cultured in flow conditions compared with static cultures. Nevertheless, when a-amylase and lipase were included in the flow perfusion cultures, the calcium content was 379 6 30 lg/scaffold after as few as 8 days. The highest calcium content (1271 6 32 lg/scaffold) was obtained for SPCL/cell constructs cultured for 16 days in the presence of lipase and flow. Furthermore, von Kossa staining and tetracycline fluorescence of histological sections demonstrated mineral deposition within the scaffolds for all groups cultured for 16 days under flow. However, all the data corroborate that lipase coupled with flow perfusion conditions improve the osteogenic differentiation of MSCs and enhance ECM mineralization. |
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Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshesStarch/poly(e-polycaprolactone) fiber meshesa-amylaseLipaseFlow perfusion bioreactorOsteogenicstarch/poly(epsilon-polycaprolactone) fiber meshesosteogenic differentiationStarch/poly(ε-polycaprolactone) fiber meshesScience & TechnologyPrevious studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteogenic differentiation of MSCs. SPCL fiber meshes were seeded with MSCs and cultured with osteogenic medium supplemented with a-amylase, lipase, or a combination of the two for 8 or 16 days using static or flow conditions. Lipase and its combination with a-amylase enhanced cell proliferation after 16 days. In addition, the flow perfusion culture enhanced the infiltration of cells and facilitated greater distribution of extracellular matrix (ECM) throughout the scaffolds in the presence/absence of enzymes. A significant amount of calcium was detected after 16 days in all groups cultured in flow conditions compared with static cultures. Nevertheless, when a-amylase and lipase were included in the flow perfusion cultures, the calcium content was 379 6 30 lg/scaffold after as few as 8 days. The highest calcium content (1271 6 32 lg/scaffold) was obtained for SPCL/cell constructs cultured for 16 days in the presence of lipase and flow. Furthermore, von Kossa staining and tetracycline fluorescence of histological sections demonstrated mineral deposition within the scaffolds for all groups cultured for 16 days under flow. However, all the data corroborate that lipase coupled with flow perfusion conditions improve the osteogenic differentiation of MSCs and enhance ECM mineralization.Contract grant sponsor: Portuguese Foundation for Science and Technology (FCT); contract grant number: SFRH/BPD/26763/2006Contract grant sponsor: European NoE EXPERTISSUES; contract grant number: NMP3-CT-2004-500283WileyUniversidade do MinhoMartins, Ana M.Saraf, A.Sousa, R. A.Alves, C. M.Mikos, Antonios G.Kasper, F. KurtisReis, R. L.2010-092010-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/14041eng1552-496510.1002/jbm.a.3278520694973info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:59Zoai:repositorium.sdum.uminho.pt:1822/14041Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:37:52.273709Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes |
title |
Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes |
spellingShingle |
Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes Martins, Ana M. Starch/poly(e-polycaprolactone) fiber meshes a-amylase Lipase Flow perfusion bioreactor Osteogenic starch/poly(epsilon-polycaprolactone) fiber meshes osteogenic differentiation Starch/poly(ε-polycaprolactone) fiber meshes Science & Technology |
title_short |
Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes |
title_full |
Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes |
title_fullStr |
Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes |
title_full_unstemmed |
Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes |
title_sort |
Combination of enzymes and flow perfusion conditions improves osteogenic differentiation of bone marrow stromal cells cultured upon starch/poly(å-caprolactone) fiber meshes |
author |
Martins, Ana M. |
author_facet |
Martins, Ana M. Saraf, A. Sousa, R. A. Alves, C. M. Mikos, Antonios G. Kasper, F. Kurtis Reis, R. L. |
author_role |
author |
author2 |
Saraf, A. Sousa, R. A. Alves, C. M. Mikos, Antonios G. Kasper, F. Kurtis Reis, R. L. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Martins, Ana M. Saraf, A. Sousa, R. A. Alves, C. M. Mikos, Antonios G. Kasper, F. Kurtis Reis, R. L. |
dc.subject.por.fl_str_mv |
Starch/poly(e-polycaprolactone) fiber meshes a-amylase Lipase Flow perfusion bioreactor Osteogenic starch/poly(epsilon-polycaprolactone) fiber meshes osteogenic differentiation Starch/poly(ε-polycaprolactone) fiber meshes Science & Technology |
topic |
Starch/poly(e-polycaprolactone) fiber meshes a-amylase Lipase Flow perfusion bioreactor Osteogenic starch/poly(epsilon-polycaprolactone) fiber meshes osteogenic differentiation Starch/poly(ε-polycaprolactone) fiber meshes Science & Technology |
description |
Previous studies have shown that a-amylase and lipase are capable of enhancing the degradation of fiber meshes blends of starch and poly(e-caprolactone) (SPCL) under dynamic conditions, and consequently to promote the proliferation and osteogenic differentiation of bone marrow stromal cells (MSCs). This study investigated the effect of flow perfusion bioreactor culture in combination with enzymes on the osteogenic differentiation of MSCs. SPCL fiber meshes were seeded with MSCs and cultured with osteogenic medium supplemented with a-amylase, lipase, or a combination of the two for 8 or 16 days using static or flow conditions. Lipase and its combination with a-amylase enhanced cell proliferation after 16 days. In addition, the flow perfusion culture enhanced the infiltration of cells and facilitated greater distribution of extracellular matrix (ECM) throughout the scaffolds in the presence/absence of enzymes. A significant amount of calcium was detected after 16 days in all groups cultured in flow conditions compared with static cultures. Nevertheless, when a-amylase and lipase were included in the flow perfusion cultures, the calcium content was 379 6 30 lg/scaffold after as few as 8 days. The highest calcium content (1271 6 32 lg/scaffold) was obtained for SPCL/cell constructs cultured for 16 days in the presence of lipase and flow. Furthermore, von Kossa staining and tetracycline fluorescence of histological sections demonstrated mineral deposition within the scaffolds for all groups cultured for 16 days under flow. However, all the data corroborate that lipase coupled with flow perfusion conditions improve the osteogenic differentiation of MSCs and enhance ECM mineralization. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09 2010-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/14041 |
url |
https://hdl.handle.net/1822/14041 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1552-4965 10.1002/jbm.a.32785 20694973 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132913950785536 |