Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxide

Detalhes bibliográficos
Autor(a) principal: Costa-Almeida, R
Data de Publicação: 2020
Outros Autores: Bogas, D, Fernandes, JR, Timochenco, L, Silva, FALS, Meneses, J, Gonçalves, IC, Magalhães, FD, Pinto, AM
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/143548
Resumo: Using a one-step thermal reduction and non-covalent chemical functionalization process, PEGylated reduced nanographene oxide (rGOn-PEG) was produced from nanographene oxide (GOn) and characterized in terms of particle size, dispersion stability, chemistry, and photothermal properties, in view of its use for photothermal therapy (PTT) of non-melanoma skin cancer. GOn infrared spectrum presented more intense bands assigned to oxygen containing functional groups than observed for rGOn-PEG. GOn C/O ratio decreased more than 50% comparing with rGOn-PEG and nitrogen was present in the latter (N at % = 20.6) due to introduction of PEG-NH2. Thermogravimetric analysis allowed estimating the amount of PEG in rGOn-PEG to be of about 56.1%. Simultaneous reduction and PEGylation increased the lateral dimensions from 287 ± 139 nm to 521 ± 397 nm, as observed by transmission electron microscopy and dynamic light scattering. rGOn-PEG exhibited ˜ 13-fold higher absorbance in the near-infrared radiation (NIR) region, as compared to unmodified GOn. Low power (150 mW cm-2) NIR irradiation using LEDs resulted in rGOn-PEG heating up to 47 °C, which is within the mild PTT temperature range. PEGylation strongly enhanced the dispersibility of rGOn in physiological media (phosphate buffered saline, fetal bovine serum, and cell culture medium) and also improved the biocompatibility of rGOn-PEG, in comparison to GOn (25-250 µg mL-1). After a single NIR LED irradiation treatment of 30 min, a decrease of ˜38% in A-431 cells viability was observed for rGOn-PEG (250 µg mL-1). Together, our results demonstrate the potential of irradiating rGOn-PEG using lower energy, cheaper, smaller, and safer LEDs, as alternative to high power lasers, for NIR mild hyperthermia therapy of cancer, namely non-melanoma skin cancer.
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spelling Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxideGrapheneLight emitting diodePhototherapyPolyethylene glycolThermal reductionUsing a one-step thermal reduction and non-covalent chemical functionalization process, PEGylated reduced nanographene oxide (rGOn-PEG) was produced from nanographene oxide (GOn) and characterized in terms of particle size, dispersion stability, chemistry, and photothermal properties, in view of its use for photothermal therapy (PTT) of non-melanoma skin cancer. GOn infrared spectrum presented more intense bands assigned to oxygen containing functional groups than observed for rGOn-PEG. GOn C/O ratio decreased more than 50% comparing with rGOn-PEG and nitrogen was present in the latter (N at % = 20.6) due to introduction of PEG-NH2. Thermogravimetric analysis allowed estimating the amount of PEG in rGOn-PEG to be of about 56.1%. Simultaneous reduction and PEGylation increased the lateral dimensions from 287 ± 139 nm to 521 ± 397 nm, as observed by transmission electron microscopy and dynamic light scattering. rGOn-PEG exhibited ˜ 13-fold higher absorbance in the near-infrared radiation (NIR) region, as compared to unmodified GOn. Low power (150 mW cm-2) NIR irradiation using LEDs resulted in rGOn-PEG heating up to 47 °C, which is within the mild PTT temperature range. PEGylation strongly enhanced the dispersibility of rGOn in physiological media (phosphate buffered saline, fetal bovine serum, and cell culture medium) and also improved the biocompatibility of rGOn-PEG, in comparison to GOn (25-250 µg mL-1). After a single NIR LED irradiation treatment of 30 min, a decrease of ˜38% in A-431 cells viability was observed for rGOn-PEG (250 µg mL-1). Together, our results demonstrate the potential of irradiating rGOn-PEG using lower energy, cheaper, smaller, and safer LEDs, as alternative to high power lasers, for NIR mild hyperthermia therapy of cancer, namely non-melanoma skin cancer.MDPI20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/143548eng2073-436010.3390/POLYM12081840Costa-Almeida, RBogas, DFernandes, JRTimochenco, LSilva, FALSMeneses, JGonçalves, ICMagalhães, FDPinto, AMinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-09-27T09:27:08Zoai:repositorio-aberto.up.pt:10216/143548Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-09-27T09:27:08Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxide
title Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxide
spellingShingle Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxide
Costa-Almeida, R
Graphene
Light emitting diode
Phototherapy
Polyethylene glycol
Thermal reduction
title_short Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxide
title_full Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxide
title_fullStr Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxide
title_full_unstemmed Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxide
title_sort Near-infrared radiation-based mild photohyperthermia therapy of non-melanoma skin cancer with PEGylated reduced nanographene oxide
author Costa-Almeida, R
author_facet Costa-Almeida, R
Bogas, D
Fernandes, JR
Timochenco, L
Silva, FALS
Meneses, J
Gonçalves, IC
Magalhães, FD
Pinto, AM
author_role author
author2 Bogas, D
Fernandes, JR
Timochenco, L
Silva, FALS
Meneses, J
Gonçalves, IC
Magalhães, FD
Pinto, AM
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa-Almeida, R
Bogas, D
Fernandes, JR
Timochenco, L
Silva, FALS
Meneses, J
Gonçalves, IC
Magalhães, FD
Pinto, AM
dc.subject.por.fl_str_mv Graphene
Light emitting diode
Phototherapy
Polyethylene glycol
Thermal reduction
topic Graphene
Light emitting diode
Phototherapy
Polyethylene glycol
Thermal reduction
description Using a one-step thermal reduction and non-covalent chemical functionalization process, PEGylated reduced nanographene oxide (rGOn-PEG) was produced from nanographene oxide (GOn) and characterized in terms of particle size, dispersion stability, chemistry, and photothermal properties, in view of its use for photothermal therapy (PTT) of non-melanoma skin cancer. GOn infrared spectrum presented more intense bands assigned to oxygen containing functional groups than observed for rGOn-PEG. GOn C/O ratio decreased more than 50% comparing with rGOn-PEG and nitrogen was present in the latter (N at % = 20.6) due to introduction of PEG-NH2. Thermogravimetric analysis allowed estimating the amount of PEG in rGOn-PEG to be of about 56.1%. Simultaneous reduction and PEGylation increased the lateral dimensions from 287 ± 139 nm to 521 ± 397 nm, as observed by transmission electron microscopy and dynamic light scattering. rGOn-PEG exhibited ˜ 13-fold higher absorbance in the near-infrared radiation (NIR) region, as compared to unmodified GOn. Low power (150 mW cm-2) NIR irradiation using LEDs resulted in rGOn-PEG heating up to 47 °C, which is within the mild PTT temperature range. PEGylation strongly enhanced the dispersibility of rGOn in physiological media (phosphate buffered saline, fetal bovine serum, and cell culture medium) and also improved the biocompatibility of rGOn-PEG, in comparison to GOn (25-250 µg mL-1). After a single NIR LED irradiation treatment of 30 min, a decrease of ˜38% in A-431 cells viability was observed for rGOn-PEG (250 µg mL-1). Together, our results demonstrate the potential of irradiating rGOn-PEG using lower energy, cheaper, smaller, and safer LEDs, as alternative to high power lasers, for NIR mild hyperthermia therapy of cancer, namely non-melanoma skin cancer.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/143548
url https://hdl.handle.net/10216/143548
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2073-4360
10.3390/POLYM12081840
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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