In vitro anti-Candida activity of Glycyrrhiza glabra L.

Detalhes bibliográficos
Autor(a) principal: Martins, Natália
Data de Publicação: 2016
Outros Autores: Ferreira, Isabel C.F.R., Henriques, Mariana, Silva, Sónia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/13336
Resumo: The severity and frequency of opportunistic fungal infections still growing, concomitantly to the increasing rates of antimicrobial drug’s resistance. Natural matrices have been used over years due to its multitude of health benefits, including antifungal potential. Thus, the present work aims to evaluate the anti-Candida potential of the phenolic extract and individual phenolic compounds of Glycyrrhiza glabra L. (licorice), by disc diffusion assay, followed by determination of the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) for both planktonic cells and biofilms. Licorice extract evidenced inhibitory potential against the nineteen tested Candida strains, but no pronounced effect was observed by testing the most abundant individual phenolic compounds. Candida tropicalis strains were the most sensible, followed by Candida glabrata, Candida parapsilosis and, then, Candida albicans. Lower MIC and MFC values were achieved to C. glabrata and C. tropicalis, which confirms its susceptibility to licorice extract; however, for C. tropicalis strains a higher variability was observed. Anti-biofilm potential was also achieved, being most evident in some C. glabrata and C. tropicalis strains. In general, a twice concentration of the MIC was necessary for planktonic cells to obtain a similar potential to that one observed for biofilms. Thus, an upcoming approach for new antifungal agents, more effective and safer than the current ones, is stablished; notwithstanding, further studies are necessary in order to understand its mechanism of action, as also to assess kinetic parameters.
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spelling In vitro anti-Candida activity of Glycyrrhiza glabra L.Glycyrrhiza glabra L.Anti-Candida potentialBiofilmsPhenolic extracts/compoundsThe severity and frequency of opportunistic fungal infections still growing, concomitantly to the increasing rates of antimicrobial drug’s resistance. Natural matrices have been used over years due to its multitude of health benefits, including antifungal potential. Thus, the present work aims to evaluate the anti-Candida potential of the phenolic extract and individual phenolic compounds of Glycyrrhiza glabra L. (licorice), by disc diffusion assay, followed by determination of the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) for both planktonic cells and biofilms. Licorice extract evidenced inhibitory potential against the nineteen tested Candida strains, but no pronounced effect was observed by testing the most abundant individual phenolic compounds. Candida tropicalis strains were the most sensible, followed by Candida glabrata, Candida parapsilosis and, then, Candida albicans. Lower MIC and MFC values were achieved to C. glabrata and C. tropicalis, which confirms its susceptibility to licorice extract; however, for C. tropicalis strains a higher variability was observed. Anti-biofilm potential was also achieved, being most evident in some C. glabrata and C. tropicalis strains. In general, a twice concentration of the MIC was necessary for planktonic cells to obtain a similar potential to that one observed for biofilms. Thus, an upcoming approach for new antifungal agents, more effective and safer than the current ones, is stablished; notwithstanding, further studies are necessary in order to understand its mechanism of action, as also to assess kinetic parameters.The authors are grateful to Foundation for Science and Technology (FCT, Portugal) for N. Martins grant (SFRH/BD/87658/2012) and financial support to CIMO (strategic project PEst-OE/AGR/UI0690/2014). This work was also supported by the Programa Operacional, Fatores de competitividade – COMPETE and by national funds through FCT on the scope of the projects FCT PTDC/SAU-MIC/119069/2010, RECI/EBB-EBI/0179/2012 and PEst-OE/EQB/LA0023/2013. The authors thank the Project “BioHealth - Biotechnology and Bioengineering approaches to improve health quality", Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 – O Novo Norte), QREN, FEDER.ElsevierBiblioteca Digital do IPBMartins, NatáliaFerreira, Isabel C.F.R.Henriques, MarianaSilva, Sónia2016-09-29T10:15:39Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10198/13336engMartins, Natália; Ferreira, Isabel C.F.R.; Henriques, Mariana; Silva, Sónia (2016). In vitro anti-Candida activity of Glycyrrhiza glabra L. Industrial Crops and Products. ISSN 0926-6690. 83, p. 81-850926-669010.1016/j.indcrop.2015.12.029info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:31:10Zoai:bibliotecadigital.ipb.pt:10198/13336Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:03:25.842127Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro anti-Candida activity of Glycyrrhiza glabra L.
title In vitro anti-Candida activity of Glycyrrhiza glabra L.
spellingShingle In vitro anti-Candida activity of Glycyrrhiza glabra L.
Martins, Natália
Glycyrrhiza glabra L.
Anti-Candida potential
Biofilms
Phenolic extracts/compounds
title_short In vitro anti-Candida activity of Glycyrrhiza glabra L.
title_full In vitro anti-Candida activity of Glycyrrhiza glabra L.
title_fullStr In vitro anti-Candida activity of Glycyrrhiza glabra L.
title_full_unstemmed In vitro anti-Candida activity of Glycyrrhiza glabra L.
title_sort In vitro anti-Candida activity of Glycyrrhiza glabra L.
author Martins, Natália
author_facet Martins, Natália
Ferreira, Isabel C.F.R.
Henriques, Mariana
Silva, Sónia
author_role author
author2 Ferreira, Isabel C.F.R.
Henriques, Mariana
Silva, Sónia
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Martins, Natália
Ferreira, Isabel C.F.R.
Henriques, Mariana
Silva, Sónia
dc.subject.por.fl_str_mv Glycyrrhiza glabra L.
Anti-Candida potential
Biofilms
Phenolic extracts/compounds
topic Glycyrrhiza glabra L.
Anti-Candida potential
Biofilms
Phenolic extracts/compounds
description The severity and frequency of opportunistic fungal infections still growing, concomitantly to the increasing rates of antimicrobial drug’s resistance. Natural matrices have been used over years due to its multitude of health benefits, including antifungal potential. Thus, the present work aims to evaluate the anti-Candida potential of the phenolic extract and individual phenolic compounds of Glycyrrhiza glabra L. (licorice), by disc diffusion assay, followed by determination of the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) for both planktonic cells and biofilms. Licorice extract evidenced inhibitory potential against the nineteen tested Candida strains, but no pronounced effect was observed by testing the most abundant individual phenolic compounds. Candida tropicalis strains were the most sensible, followed by Candida glabrata, Candida parapsilosis and, then, Candida albicans. Lower MIC and MFC values were achieved to C. glabrata and C. tropicalis, which confirms its susceptibility to licorice extract; however, for C. tropicalis strains a higher variability was observed. Anti-biofilm potential was also achieved, being most evident in some C. glabrata and C. tropicalis strains. In general, a twice concentration of the MIC was necessary for planktonic cells to obtain a similar potential to that one observed for biofilms. Thus, an upcoming approach for new antifungal agents, more effective and safer than the current ones, is stablished; notwithstanding, further studies are necessary in order to understand its mechanism of action, as also to assess kinetic parameters.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-29T10:15:39Z
2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/13336
url http://hdl.handle.net/10198/13336
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Martins, Natália; Ferreira, Isabel C.F.R.; Henriques, Mariana; Silva, Sónia (2016). In vitro anti-Candida activity of Glycyrrhiza glabra L. Industrial Crops and Products. ISSN 0926-6690. 83, p. 81-85
0926-6690
10.1016/j.indcrop.2015.12.029
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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