Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactors

Detalhes bibliográficos
Autor(a) principal: Domingos, Joana Margarida Bendada
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/10648
Resumo: Dissertation for the Master degree in Biotechnology
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spelling Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactorsAcidogenic mixed cultureCeramic supportCheese wheyPacked-bed bioreactorVolatile fatty acidsDissertation for the Master degree in BiotechnologyThe main goal of the present work was to study the production of volatile fatty acids (VFAs) from cheese whey powder (CWP) by employing a packed bed bioreactor (PPBR) for the anaerobic acidogenesis. First experiments were performed in 100-mL Pyrex bottles to study the acidogenesis trends, namely: lactose consumption, VFAs and biogas production and composition. These tests were done with freely suspended-cells (control experiment) and with immobilized cells using granular activated carbon (AC) and ceramic cube Vukopor S10 supports. The utilized inoculum – an acidogenic mix consortium- belongs to an analogous CWP digestion process in which a different culture system is being studied. Therefore, the incubations conditions were the same as for that culture system: 20 g/L of CWP (corresponding to 15 g/L lactose), 37ºC and pH 6. The observed trend consisted on lactose consumption, lactic acid formation (as an intermediate product) and from this VFAs production. The best yield was obtained when Vukopor was used (87% against 30% for AC); after 9 days the VFAs was (g/L): acetic (1.6), propionic (2.4); butyric (6.6) acids. The mentioned preliminary studies allowed selecting the operational hydraulic retention time(HRT) for the bioreactors. Two recirculate 1-liter PBBR one filled with Vukopor and other with AC were developed. CWP concentration, pH and temperature were the same as in the microcosm experiment. Both were operated in batch and continuous. In first batch performed in PBBR-Vuko it was achieved 6 g/L of propionic. However a loss of capability of producing it was observed during continuous operation. It was ascribed to a wash-out of related strains. With PBBR-Vuko were tested two different hydraulic retention times (HRT), 9 and 6 days, instead for PBBR-AC only HRT of 9 days. The yields for PBBRVuko were the same as at the microcosms scale, 80% for both HRT. On the other hand, the yield for PBBR-AC was 20%, this is a confirmation that AC was not the proper support even at a 1-L scale. Additionally to immobilization study, it was also set up a bioreactor with freely suspended cells. In this last mentioned bioreactor when a HRT of 6 days was set up it was observed a decrease in the VFAs yield to 44%. From this, it was concluded that the immobilization is an advantage for the VFAs production.Faculdade de Ciências e TecnologiaFava, FabioBertin, LorenzoRUNDomingos, Joana Margarida Bendada2013-10-29T11:57:32Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10648TID:201753758enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:44:32Zoai:run.unl.pt:10362/10648Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:19:38.712412Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactors
title Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactors
spellingShingle Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactors
Domingos, Joana Margarida Bendada
Acidogenic mixed culture
Ceramic support
Cheese whey
Packed-bed bioreactor
Volatile fatty acids
title_short Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactors
title_full Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactors
title_fullStr Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactors
title_full_unstemmed Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactors
title_sort Acidogenic digestion of effluents of the cheese industry in packed bed biofilm reactors
author Domingos, Joana Margarida Bendada
author_facet Domingos, Joana Margarida Bendada
author_role author
dc.contributor.none.fl_str_mv Fava, Fabio
Bertin, Lorenzo
RUN
dc.contributor.author.fl_str_mv Domingos, Joana Margarida Bendada
dc.subject.por.fl_str_mv Acidogenic mixed culture
Ceramic support
Cheese whey
Packed-bed bioreactor
Volatile fatty acids
topic Acidogenic mixed culture
Ceramic support
Cheese whey
Packed-bed bioreactor
Volatile fatty acids
description Dissertation for the Master degree in Biotechnology
publishDate 2013
dc.date.none.fl_str_mv 2013-10-29T11:57:32Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10648
TID:201753758
url http://hdl.handle.net/10362/10648
identifier_str_mv TID:201753758
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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