Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection

Detalhes bibliográficos
Autor(a) principal: Soares, R. S.
Data de Publicação: 2011
Outros Autores: Matoso, P., Calado, M., Sousa, A. E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/18300
Resumo: © 2011 Elsevier B.V. All rights reserved.
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spelling Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infectionAIDSHIV-2Unspliced mRNAMultiply spliced mRNAHIV-2 replicationOne-step RT-qPCR© 2011 Elsevier B.V. All rights reserved.HIV-2 infection is associated with a slower rate of disease progression with limited impact on the survival of the majority of infected adults, and much lower plasma viral load than HIV-1. In spite of the major differences in viremia, the quantitative assessment of HIV-2 proviral load documented levels similar to those observed in HIV-1 infected individuals, suggesting an equivalent number of circulating infected cells in both infections. It remains unclear whether this apparent paradox results from a contribution of latent/quiescent viruses or from transcriptional and/or post-transcriptional control of HIV-2 replication. In order to investigate these possibilities, a one-step and two-step reverse transcription quantitative real-time PCR based methods (RT-qPCR) for gag and tat mRNA HIV-2 transcripts were developed. These methods were validated and compared to assess the expression of HIV-2 gag and tat transcripts in parallel with proviral DNA and viral production. The results suggest that the two-step approach may allow a better detection of low level gag and tat mRNA HIV-2 transcripts.Grants from “Fundação para a Ciência e a Tecnologia” (FCT) and “Programa Operacional Ciência e Inovação 2010” (POCI2010) as well as from Fundação Calouste Gulbenkian to A.E.S. R.S.S., P.M., and M.C. received scholarships from FCT.ElsevierRepositório da Universidade de LisboaSoares, R. S.Matoso, P.Calado, M.Sousa, A. E.2015-06-17T11:56:57Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/18300engJournal of Virological Methods 175 (2011) 38– 450166-0934http://www.sciencedirect.com/science/article/pii/S0166093411001509#metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:23:02Zoai:repositorio.ul.pt:10451/18300Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:23:02Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection
title Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection
spellingShingle Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection
Soares, R. S.
AIDS
HIV-2
Unspliced mRNA
Multiply spliced mRNA
HIV-2 replication
One-step RT-qPCR
title_short Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection
title_full Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection
title_fullStr Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection
title_full_unstemmed Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection
title_sort Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection
author Soares, R. S.
author_facet Soares, R. S.
Matoso, P.
Calado, M.
Sousa, A. E.
author_role author
author2 Matoso, P.
Calado, M.
Sousa, A. E.
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Soares, R. S.
Matoso, P.
Calado, M.
Sousa, A. E.
dc.subject.por.fl_str_mv AIDS
HIV-2
Unspliced mRNA
Multiply spliced mRNA
HIV-2 replication
One-step RT-qPCR
topic AIDS
HIV-2
Unspliced mRNA
Multiply spliced mRNA
HIV-2 replication
One-step RT-qPCR
description © 2011 Elsevier B.V. All rights reserved.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2015-06-17T11:56:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/18300
url http://hdl.handle.net/10451/18300
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Virological Methods 175 (2011) 38– 45
0166-0934
http://www.sciencedirect.com/science/article/pii/S0166093411001509#
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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