Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/18300 |
Resumo: | © 2011 Elsevier B.V. All rights reserved. |
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Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infectionAIDSHIV-2Unspliced mRNAMultiply spliced mRNAHIV-2 replicationOne-step RT-qPCR© 2011 Elsevier B.V. All rights reserved.HIV-2 infection is associated with a slower rate of disease progression with limited impact on the survival of the majority of infected adults, and much lower plasma viral load than HIV-1. In spite of the major differences in viremia, the quantitative assessment of HIV-2 proviral load documented levels similar to those observed in HIV-1 infected individuals, suggesting an equivalent number of circulating infected cells in both infections. It remains unclear whether this apparent paradox results from a contribution of latent/quiescent viruses or from transcriptional and/or post-transcriptional control of HIV-2 replication. In order to investigate these possibilities, a one-step and two-step reverse transcription quantitative real-time PCR based methods (RT-qPCR) for gag and tat mRNA HIV-2 transcripts were developed. These methods were validated and compared to assess the expression of HIV-2 gag and tat transcripts in parallel with proviral DNA and viral production. The results suggest that the two-step approach may allow a better detection of low level gag and tat mRNA HIV-2 transcripts.Grants from “Fundação para a Ciência e a Tecnologia” (FCT) and “Programa Operacional Ciência e Inovação 2010” (POCI2010) as well as from Fundação Calouste Gulbenkian to A.E.S. R.S.S., P.M., and M.C. received scholarships from FCT.ElsevierRepositório da Universidade de LisboaSoares, R. S.Matoso, P.Calado, M.Sousa, A. E.2015-06-17T11:56:57Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/18300engJournal of Virological Methods 175 (2011) 38– 450166-0934http://www.sciencedirect.com/science/article/pii/S0166093411001509#metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:23:02Zoai:repositorio.ul.pt:10451/18300Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:23:02Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection |
title |
Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection |
spellingShingle |
Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection Soares, R. S. AIDS HIV-2 Unspliced mRNA Multiply spliced mRNA HIV-2 replication One-step RT-qPCR |
title_short |
Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection |
title_full |
Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection |
title_fullStr |
Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection |
title_full_unstemmed |
Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection |
title_sort |
Strategies to quantify unspliced and multiply spliced mRNA expression in HIV-2 infection |
author |
Soares, R. S. |
author_facet |
Soares, R. S. Matoso, P. Calado, M. Sousa, A. E. |
author_role |
author |
author2 |
Matoso, P. Calado, M. Sousa, A. E. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Soares, R. S. Matoso, P. Calado, M. Sousa, A. E. |
dc.subject.por.fl_str_mv |
AIDS HIV-2 Unspliced mRNA Multiply spliced mRNA HIV-2 replication One-step RT-qPCR |
topic |
AIDS HIV-2 Unspliced mRNA Multiply spliced mRNA HIV-2 replication One-step RT-qPCR |
description |
© 2011 Elsevier B.V. All rights reserved. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z 2015-06-17T11:56:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/18300 |
url |
http://hdl.handle.net/10451/18300 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Virological Methods 175 (2011) 38– 45 0166-0934 http://www.sciencedirect.com/science/article/pii/S0166093411001509# |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817548850145001472 |