Flower development and sex specification in wild grapevine

Detalhes bibliográficos
Autor(a) principal: Ramos, Miguel Jesus Nunes
Data de Publicação: 2014
Outros Autores: Coito, João Lucas, Silva, Helena Gomes, Cunha, Jorge, Costa, Maria Manuela Ribeiro, Rocheta, Margarida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/8708
Resumo: Research Article
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spelling Flower development and sex specification in wild grapevineVitis sylvestrisflowerdevelopmentsexRNA-Seqintronic RNAResearch ArticleBackground: Wild plants of Vitis closely related to the cultivated grapevine (V. v. vinifera) are believed to have been first domesticated 10,000 years BC around the Caspian Sea. V. v. vinifera is hermaphrodite whereas V. v. sylvestris is a dioecious species. Male flowers show a reduced pistil without style or stigma and female flowers present reflexed stamens with infertile pollen. V. vinifera produce perfect flowers with all functional structures. The mechanism for flower sex determination and specification in grapevine is still unknown. Results: To understand which genes are involved during the establishment of male, female and complete flowers, we analysed and compared the transcription profiles of four developmental stages of the three genders. We showed that sex determination is a late event during flower development and that the expression of genes from the ABCDE model is not directly correlated with the establishment of sexual dimorphism. We propose a temporal comprehensive model in which two mutations in two linked genes could be players in sex determination and indirectly establish the Vitis domestication process. Additionally, we also found clusters of genes differentially expressed between genders and between developmental stages that suggest a role involved in sex differentiation. Also, the detection of differentially transcribed regions that extended existing gene models (intergenic regions) between sexes suggests that they may account for some of the variation between the subspecies. Conclusions: There is no evidence of differences of expression levels in genes from the ABCDE model that could explain the shift from hermaphroditism to dioecy. We propose that sex specification occurs after floral organ identity has been established and therefore, sex determination genes might be having an effect downstream of the ABCDE model genes. For the first time a full transcriptomic analysis was performed in different flower developmental stages in the same individual. Our experimental approach enabled us to create a comprehensive catalogue of transcribed genes across developmental stages and genders that will contribute for future work in sex determination in seed plantsBioMed CentralRepositório da Universidade de LisboaRamos, Miguel Jesus NunesCoito, João LucasSilva, Helena GomesCunha, JorgeCosta, Maria Manuela RibeiroRocheta, Margarida2015-06-02T09:42:55Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/8708engRamos et al.: Flower development and sex specification in wild grapevine. BMC Genomics 2014 15:1095info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:39:16Zoai:www.repository.utl.pt:10400.5/8708Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:55:38.868330Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Flower development and sex specification in wild grapevine
title Flower development and sex specification in wild grapevine
spellingShingle Flower development and sex specification in wild grapevine
Ramos, Miguel Jesus Nunes
Vitis sylvestris
flower
development
sex
RNA-Seq
intronic RNA
title_short Flower development and sex specification in wild grapevine
title_full Flower development and sex specification in wild grapevine
title_fullStr Flower development and sex specification in wild grapevine
title_full_unstemmed Flower development and sex specification in wild grapevine
title_sort Flower development and sex specification in wild grapevine
author Ramos, Miguel Jesus Nunes
author_facet Ramos, Miguel Jesus Nunes
Coito, João Lucas
Silva, Helena Gomes
Cunha, Jorge
Costa, Maria Manuela Ribeiro
Rocheta, Margarida
author_role author
author2 Coito, João Lucas
Silva, Helena Gomes
Cunha, Jorge
Costa, Maria Manuela Ribeiro
Rocheta, Margarida
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Ramos, Miguel Jesus Nunes
Coito, João Lucas
Silva, Helena Gomes
Cunha, Jorge
Costa, Maria Manuela Ribeiro
Rocheta, Margarida
dc.subject.por.fl_str_mv Vitis sylvestris
flower
development
sex
RNA-Seq
intronic RNA
topic Vitis sylvestris
flower
development
sex
RNA-Seq
intronic RNA
description Research Article
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
2015-06-02T09:42:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/8708
url http://hdl.handle.net/10400.5/8708
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ramos et al.: Flower development and sex specification in wild grapevine. BMC Genomics 2014 15:1095
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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