Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Matéria (Rio de Janeiro. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-70762019000200323 |
Resumo: | ABSTRACT Due to its high carbohydrate content, algae biomass can be employed as feedstock to produce hydrogen (H2) by fermentation. However, to make the carbohydrates entrapped within the cell wall more bioavailable, algae biomass must be treated before fermentation. We submitted Kappaphyccus alvarezzi macroalgae biomass to autoclave (at 120 °C and 1 atm for 6 h) treatment and/or enzymatic (Celluclast® and/or a recombinant β-glucosidase) hydrolysis, to break down complex carbohydrates into available sugars that were used to produce H2 by fermentation. Macroalgae biomass treated with Celluclast®+β-glucosidase and with combined thermal treatment and enzymatic hydrolysis reached very similar TRS productivities, 0.24 and 0.22 g of TRS/L.h, respectively. The enzymatically treated biomass was employed as feedstock to produce H2 by Clostridium beijerinckii Br21, which afforded high yield: 21.3 mmol of H2/g of dry algae biomass. Hence, treatment with Celluclast® and recombinant β-glucosidase provided macroalgae biomass for enhanced bioconversion to H2 by C. beijerinckii Br21. |
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Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 productionKappaphyccus alvarezziClostridium beijerinckiiBiohydrogenCellulasβ-glucosidaseABSTRACT Due to its high carbohydrate content, algae biomass can be employed as feedstock to produce hydrogen (H2) by fermentation. However, to make the carbohydrates entrapped within the cell wall more bioavailable, algae biomass must be treated before fermentation. We submitted Kappaphyccus alvarezzi macroalgae biomass to autoclave (at 120 °C and 1 atm for 6 h) treatment and/or enzymatic (Celluclast® and/or a recombinant β-glucosidase) hydrolysis, to break down complex carbohydrates into available sugars that were used to produce H2 by fermentation. Macroalgae biomass treated with Celluclast®+β-glucosidase and with combined thermal treatment and enzymatic hydrolysis reached very similar TRS productivities, 0.24 and 0.22 g of TRS/L.h, respectively. The enzymatically treated biomass was employed as feedstock to produce H2 by Clostridium beijerinckii Br21, which afforded high yield: 21.3 mmol of H2/g of dry algae biomass. Hence, treatment with Celluclast® and recombinant β-glucosidase provided macroalgae biomass for enhanced bioconversion to H2 by C. beijerinckii Br21.Laboratório de Hidrogênio, Coppe - Universidade Federal do Rio de Janeiroem cooperação com a Associação Brasileira do Hidrogênio, ABH22019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-70762019000200323Matéria (Rio de Janeiro) v.24 n.2 2019reponame:Matéria (Rio de Janeiro. Online)instname:Matéria (Rio de Janeiro. Online)instacron:RLAM10.1590/s1517-707620190002.0678info:eu-repo/semantics/openAccessRodrigues,Emelin LeandroFonseca,Bruna ConstanteGelli,Valeria CressCarli,SibeliMeleiro,Luana ParrasFurriel,Rosa Prazeres MeloReginatto,Valeriaeng2020-07-22T00:00:00Zoai:scielo:S1517-70762019000200323Revistahttp://www.materia.coppe.ufrj.br/https://old.scielo.br/oai/scielo-oai.php||materia@labh2.coppe.ufrj.br1517-70761517-7076opendoar:2020-07-22T00:00Matéria (Rio de Janeiro. Online) - Matéria (Rio de Janeiro. Online)false |
dc.title.none.fl_str_mv |
Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production |
title |
Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production |
spellingShingle |
Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production Rodrigues,Emelin Leandro Kappaphyccus alvarezzi Clostridium beijerinckii Biohydrogen Cellulas β-glucosidase |
title_short |
Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production |
title_full |
Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production |
title_fullStr |
Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production |
title_full_unstemmed |
Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production |
title_sort |
Enzymatically and/or thermally treated Macroalgae biomass as feedstock for fermentative H2 production |
author |
Rodrigues,Emelin Leandro |
author_facet |
Rodrigues,Emelin Leandro Fonseca,Bruna Constante Gelli,Valeria Cress Carli,Sibeli Meleiro,Luana Parras Furriel,Rosa Prazeres Melo Reginatto,Valeria |
author_role |
author |
author2 |
Fonseca,Bruna Constante Gelli,Valeria Cress Carli,Sibeli Meleiro,Luana Parras Furriel,Rosa Prazeres Melo Reginatto,Valeria |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues,Emelin Leandro Fonseca,Bruna Constante Gelli,Valeria Cress Carli,Sibeli Meleiro,Luana Parras Furriel,Rosa Prazeres Melo Reginatto,Valeria |
dc.subject.por.fl_str_mv |
Kappaphyccus alvarezzi Clostridium beijerinckii Biohydrogen Cellulas β-glucosidase |
topic |
Kappaphyccus alvarezzi Clostridium beijerinckii Biohydrogen Cellulas β-glucosidase |
description |
ABSTRACT Due to its high carbohydrate content, algae biomass can be employed as feedstock to produce hydrogen (H2) by fermentation. However, to make the carbohydrates entrapped within the cell wall more bioavailable, algae biomass must be treated before fermentation. We submitted Kappaphyccus alvarezzi macroalgae biomass to autoclave (at 120 °C and 1 atm for 6 h) treatment and/or enzymatic (Celluclast® and/or a recombinant β-glucosidase) hydrolysis, to break down complex carbohydrates into available sugars that were used to produce H2 by fermentation. Macroalgae biomass treated with Celluclast®+β-glucosidase and with combined thermal treatment and enzymatic hydrolysis reached very similar TRS productivities, 0.24 and 0.22 g of TRS/L.h, respectively. The enzymatically treated biomass was employed as feedstock to produce H2 by Clostridium beijerinckii Br21, which afforded high yield: 21.3 mmol of H2/g of dry algae biomass. Hence, treatment with Celluclast® and recombinant β-glucosidase provided macroalgae biomass for enhanced bioconversion to H2 by C. beijerinckii Br21. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-70762019000200323 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-70762019000200323 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s1517-707620190002.0678 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Laboratório de Hidrogênio, Coppe - Universidade Federal do Rio de Janeiro em cooperação com a Associação Brasileira do Hidrogênio, ABH2 |
publisher.none.fl_str_mv |
Laboratório de Hidrogênio, Coppe - Universidade Federal do Rio de Janeiro em cooperação com a Associação Brasileira do Hidrogênio, ABH2 |
dc.source.none.fl_str_mv |
Matéria (Rio de Janeiro) v.24 n.2 2019 reponame:Matéria (Rio de Janeiro. Online) instname:Matéria (Rio de Janeiro. Online) instacron:RLAM |
instname_str |
Matéria (Rio de Janeiro. Online) |
instacron_str |
RLAM |
institution |
RLAM |
reponame_str |
Matéria (Rio de Janeiro. Online) |
collection |
Matéria (Rio de Janeiro. Online) |
repository.name.fl_str_mv |
Matéria (Rio de Janeiro. Online) - Matéria (Rio de Janeiro. Online) |
repository.mail.fl_str_mv |
||materia@labh2.coppe.ufrj.br |
_version_ |
1752126691992403968 |