Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Anestesiologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-70942015000100021 |
Resumo: | BACKGROUND AND OBJECTIVE: Previous studies regarding the effects of some local anaesthetics have suggested that these agents can cause genetic damage. However, they have not been tested for genotoxicity related to repetitive administration. The aim of this study was to evaluate the genotoxic potential of local anaesthetics upon repetitive administration. METHODS: 80 male Wistar rats were divided into: group A - 16 rats intraperitoneally injected with lidocaine hydrochloride 2%; group B - 16 rats IP injected with mepivacaine 2%; group C - 16 rats intraperitoneally injected with articaine 4%; group D - 16 rats IP injected with prilocaine 3% (6.0 mg/kg); group E - 8 rats subcutaneously injected with a single dose of cyclophosphamide; and group F - 8 rats intraperitoneally injected with saline. Eight rats from groups A to D received a single dose of anaesthetic on Day 1 of the experiment; the remaining rats were dosed once a day for 5 days. RESULTS: The median number of micronuclei in the local anaesthetics groups exposed for 1 or 5 days ranged from 0.00 to 1.00, in the cyclophosphamide-exposed group was 10.00, and the negative control group for 1 and 5 days was 1.00 and 0.00, respectively (p < 0.0001). A significant difference in the number of micronuclei was observed between the cyclophosphamide group and all local anaesthetic groups (p = 0.0001), but not between the negative control group and the local anaesthetic groups (p > 0.05). CONCLUSION: No genotoxicity effect was observed upon repetitive exposure to any of the local anaesthetics evaluated. |
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Revista Brasileira de Anestesiologia (Online) |
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Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in ratsAnaesthesiaGenotoxicityMutagenicity testsMicronucleus testsPrilocaine BACKGROUND AND OBJECTIVE: Previous studies regarding the effects of some local anaesthetics have suggested that these agents can cause genetic damage. However, they have not been tested for genotoxicity related to repetitive administration. The aim of this study was to evaluate the genotoxic potential of local anaesthetics upon repetitive administration. METHODS: 80 male Wistar rats were divided into: group A - 16 rats intraperitoneally injected with lidocaine hydrochloride 2%; group B - 16 rats IP injected with mepivacaine 2%; group C - 16 rats intraperitoneally injected with articaine 4%; group D - 16 rats IP injected with prilocaine 3% (6.0 mg/kg); group E - 8 rats subcutaneously injected with a single dose of cyclophosphamide; and group F - 8 rats intraperitoneally injected with saline. Eight rats from groups A to D received a single dose of anaesthetic on Day 1 of the experiment; the remaining rats were dosed once a day for 5 days. RESULTS: The median number of micronuclei in the local anaesthetics groups exposed for 1 or 5 days ranged from 0.00 to 1.00, in the cyclophosphamide-exposed group was 10.00, and the negative control group for 1 and 5 days was 1.00 and 0.00, respectively (p < 0.0001). A significant difference in the number of micronuclei was observed between the cyclophosphamide group and all local anaesthetic groups (p = 0.0001), but not between the negative control group and the local anaesthetic groups (p > 0.05). CONCLUSION: No genotoxicity effect was observed upon repetitive exposure to any of the local anaesthetics evaluated. Sociedade Brasileira de Anestesiologia2015-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-70942015000100021Revista Brasileira de Anestesiologia v.65 n.1 2015reponame:Revista Brasileira de Anestesiologia (Online)instname:Sociedade Brasileira de Anestesiologia (SBA)instacron:SBA10.1016/j.bjane.2013.07.006info:eu-repo/semantics/openAccessNai,Gisele AlborghettiOliveira,Mariliza Casanova deTavares,Graziela de OliveiraPereira,Laís Fabrício FonsecaSoares,Nádia Derli Salvador LemesSilva,Patrícia Gattieng2015-09-10T00:00:00Zoai:scielo:S0034-70942015000100021Revistahttps://www.sbahq.org/revista/https://old.scielo.br/oai/scielo-oai.php||sba2000@openlink.com.br1806-907X0034-7094opendoar:2015-09-10T00:00Revista Brasileira de Anestesiologia (Online) - Sociedade Brasileira de Anestesiologia (SBA)false |
dc.title.none.fl_str_mv |
Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats |
title |
Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats |
spellingShingle |
Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats Nai,Gisele Alborghetti Anaesthesia Genotoxicity Mutagenicity tests Micronucleus tests Prilocaine |
title_short |
Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats |
title_full |
Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats |
title_fullStr |
Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats |
title_full_unstemmed |
Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats |
title_sort |
Evaluation of genotoxicity induced by repetitive administration of local anaesthetics: an experimental study in rats |
author |
Nai,Gisele Alborghetti |
author_facet |
Nai,Gisele Alborghetti Oliveira,Mariliza Casanova de Tavares,Graziela de Oliveira Pereira,Laís Fabrício Fonseca Soares,Nádia Derli Salvador Lemes Silva,Patrícia Gatti |
author_role |
author |
author2 |
Oliveira,Mariliza Casanova de Tavares,Graziela de Oliveira Pereira,Laís Fabrício Fonseca Soares,Nádia Derli Salvador Lemes Silva,Patrícia Gatti |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Nai,Gisele Alborghetti Oliveira,Mariliza Casanova de Tavares,Graziela de Oliveira Pereira,Laís Fabrício Fonseca Soares,Nádia Derli Salvador Lemes Silva,Patrícia Gatti |
dc.subject.por.fl_str_mv |
Anaesthesia Genotoxicity Mutagenicity tests Micronucleus tests Prilocaine |
topic |
Anaesthesia Genotoxicity Mutagenicity tests Micronucleus tests Prilocaine |
description |
BACKGROUND AND OBJECTIVE: Previous studies regarding the effects of some local anaesthetics have suggested that these agents can cause genetic damage. However, they have not been tested for genotoxicity related to repetitive administration. The aim of this study was to evaluate the genotoxic potential of local anaesthetics upon repetitive administration. METHODS: 80 male Wistar rats were divided into: group A - 16 rats intraperitoneally injected with lidocaine hydrochloride 2%; group B - 16 rats IP injected with mepivacaine 2%; group C - 16 rats intraperitoneally injected with articaine 4%; group D - 16 rats IP injected with prilocaine 3% (6.0 mg/kg); group E - 8 rats subcutaneously injected with a single dose of cyclophosphamide; and group F - 8 rats intraperitoneally injected with saline. Eight rats from groups A to D received a single dose of anaesthetic on Day 1 of the experiment; the remaining rats were dosed once a day for 5 days. RESULTS: The median number of micronuclei in the local anaesthetics groups exposed for 1 or 5 days ranged from 0.00 to 1.00, in the cyclophosphamide-exposed group was 10.00, and the negative control group for 1 and 5 days was 1.00 and 0.00, respectively (p < 0.0001). A significant difference in the number of micronuclei was observed between the cyclophosphamide group and all local anaesthetic groups (p = 0.0001), but not between the negative control group and the local anaesthetic groups (p > 0.05). CONCLUSION: No genotoxicity effect was observed upon repetitive exposure to any of the local anaesthetics evaluated. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-70942015000100021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0034-70942015000100021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjane.2013.07.006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Anestesiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Anestesiologia |
dc.source.none.fl_str_mv |
Revista Brasileira de Anestesiologia v.65 n.1 2015 reponame:Revista Brasileira de Anestesiologia (Online) instname:Sociedade Brasileira de Anestesiologia (SBA) instacron:SBA |
instname_str |
Sociedade Brasileira de Anestesiologia (SBA) |
instacron_str |
SBA |
institution |
SBA |
reponame_str |
Revista Brasileira de Anestesiologia (Online) |
collection |
Revista Brasileira de Anestesiologia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Anestesiologia (Online) - Sociedade Brasileira de Anestesiologia (SBA) |
repository.mail.fl_str_mv |
||sba2000@openlink.com.br |
_version_ |
1752126628221157376 |