Enzymatic activity analysis of MMP-2 and 9 collected by swab from lower limb venous ulcers

• Autor(a) principal: Silva,Flávio Santos da
• Data de Publicação: 2014
• Outros Autores: Araujo,Diego Neves, Lima,João Paulo Matos Santos, Rezende,Adriana Augusto de, Abreu,Bento João da Graça Azevedo, Dias,Fernando Augusto Lavezzo
• Tipo de documento: Relatório
• Idioma: eng
• Título da fonte: Jornal Vascular Brasileiro (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1677-54492014000300229
• topic: venous ulcer; metalloproteinases; gelatinases

Metalloproteinases play a role in repair of venous ulcers of the lower limbs. The great majority of studies of metalloproteinase enzyme activity conducted to date have employed material from biopsies of ulcers. We evaluated the viability of using zymography to measure the enzyme activity of metalloproteinases 2 and 9 in samples of venous ulcer exudate collected on swabs. The method chosen for processing the samples proved viable in terms of its ability to provide adequate protein concentrations for analysis. Using zymography, we observed that the parameters that provided the best results for analysis of gelatinolytic activity were 0.125 to 0.5 μg of total protein content in the gels and enzymatic activation time of 19 hours (at 37 °C). Collection of venous ulcer fluid using swabs proved to be a simple, rapid and effective method for obtaining samples for measurement of gelatinolytic activity with a minimum degree of invasivity.