Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Botanica Brasilica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062008000300024 |
Resumo: | This work evaluated the effects of Tris (hydroxymethyl)-aminomethane (TRIS) buffer and its interaction with nutrient concentration on the development of Gracilaria birdie, a common species on the Brazilian coast that has been exploited for agar production. Responses to different conditions were assessed through growth rates and pigment content (chlorophyll a, phycoerythrin, phycocyanin and allophycocyanin). Provasoli's nutrient solution with and without TRIS addition was tested at concentrations of 12.5, 25 and 50%. The pH was also monitored. G. birdiae grew better in the absence of TRIS and at low nutrient concentrations, 12.5 and 25% (growth rates of 10.8-11.3%.day-1). Higher contents of phycoerythrin and chlorophyll a were observed without TRIS at 12.5 and 25% (Phycoerythrin, 649.6-698.0 μg g-1 fresh biomass; Chlorophyll a, 156.0-168.6 μg g-1 fresh biomass). These findings highlight the deleterious effect of TRIS on growth and phycoerythrin and chlorophyll a content. They also demonstrate the importance of appropriate nutrient concentration for laboratory cultures, depending on the intrinsic characteristics of each species. |
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Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta)culture mediumTRIS (hydroxymethyl)-aminomethaneGracilaria birdiaegrowthpigmentsThis work evaluated the effects of Tris (hydroxymethyl)-aminomethane (TRIS) buffer and its interaction with nutrient concentration on the development of Gracilaria birdie, a common species on the Brazilian coast that has been exploited for agar production. Responses to different conditions were assessed through growth rates and pigment content (chlorophyll a, phycoerythrin, phycocyanin and allophycocyanin). Provasoli's nutrient solution with and without TRIS addition was tested at concentrations of 12.5, 25 and 50%. The pH was also monitored. G. birdiae grew better in the absence of TRIS and at low nutrient concentrations, 12.5 and 25% (growth rates of 10.8-11.3%.day-1). Higher contents of phycoerythrin and chlorophyll a were observed without TRIS at 12.5 and 25% (Phycoerythrin, 649.6-698.0 μg g-1 fresh biomass; Chlorophyll a, 156.0-168.6 μg g-1 fresh biomass). These findings highlight the deleterious effect of TRIS on growth and phycoerythrin and chlorophyll a content. They also demonstrate the importance of appropriate nutrient concentration for laboratory cultures, depending on the intrinsic characteristics of each species.Sociedade Botânica do Brasil2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062008000300024Acta Botanica Brasilica v.22 n.3 2008reponame:Acta Botanica Brasilicainstname:Sociedade Botânica do Brasil (SBB)instacron:SBB10.1590/S0102-33062008000300024info:eu-repo/semantics/openAccessUrsi,SuzanaGuimarães,MelinaPlastino,Estela Mariaeng2008-11-11T00:00:00Zoai:scielo:S0102-33062008000300024Revistahttps://www.scielo.br/j/abb/ONGhttps://old.scielo.br/oai/scielo-oai.phpacta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com1677-941X0102-3306opendoar:2008-11-11T00:00Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)false |
dc.title.none.fl_str_mv |
Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta) |
title |
Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta) |
spellingShingle |
Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta) Ursi,Suzana culture medium TRIS (hydroxymethyl)-aminomethane Gracilaria birdiae growth pigments |
title_short |
Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta) |
title_full |
Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta) |
title_fullStr |
Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta) |
title_full_unstemmed |
Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta) |
title_sort |
Deleterious effect of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino & E.C. Oliveira (Gracilariales, Rhodophyta) |
author |
Ursi,Suzana |
author_facet |
Ursi,Suzana Guimarães,Melina Plastino,Estela Maria |
author_role |
author |
author2 |
Guimarães,Melina Plastino,Estela Maria |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ursi,Suzana Guimarães,Melina Plastino,Estela Maria |
dc.subject.por.fl_str_mv |
culture medium TRIS (hydroxymethyl)-aminomethane Gracilaria birdiae growth pigments |
topic |
culture medium TRIS (hydroxymethyl)-aminomethane Gracilaria birdiae growth pigments |
description |
This work evaluated the effects of Tris (hydroxymethyl)-aminomethane (TRIS) buffer and its interaction with nutrient concentration on the development of Gracilaria birdie, a common species on the Brazilian coast that has been exploited for agar production. Responses to different conditions were assessed through growth rates and pigment content (chlorophyll a, phycoerythrin, phycocyanin and allophycocyanin). Provasoli's nutrient solution with and without TRIS addition was tested at concentrations of 12.5, 25 and 50%. The pH was also monitored. G. birdiae grew better in the absence of TRIS and at low nutrient concentrations, 12.5 and 25% (growth rates of 10.8-11.3%.day-1). Higher contents of phycoerythrin and chlorophyll a were observed without TRIS at 12.5 and 25% (Phycoerythrin, 649.6-698.0 μg g-1 fresh biomass; Chlorophyll a, 156.0-168.6 μg g-1 fresh biomass). These findings highlight the deleterious effect of TRIS on growth and phycoerythrin and chlorophyll a content. They also demonstrate the importance of appropriate nutrient concentration for laboratory cultures, depending on the intrinsic characteristics of each species. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062008000300024 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062008000300024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-33062008000300024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
dc.source.none.fl_str_mv |
Acta Botanica Brasilica v.22 n.3 2008 reponame:Acta Botanica Brasilica instname:Sociedade Botânica do Brasil (SBB) instacron:SBB |
instname_str |
Sociedade Botânica do Brasil (SBB) |
instacron_str |
SBB |
institution |
SBB |
reponame_str |
Acta Botanica Brasilica |
collection |
Acta Botanica Brasilica |
repository.name.fl_str_mv |
Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB) |
repository.mail.fl_str_mv |
acta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com |
_version_ |
1752126658772467712 |